is a member of the family Enterobacteriaceae and an enigmatic plant pathogen with broad host range. Although

Burkholderia gladioli pv. alliicola, B. cepacia, and orbicola are common bacterial pathogens of onion. Onions produce organosulfur thiosulfinate defensive compounds after cellular decompartmentalization. Using whole-genome sequencing in silico analysis, we identified putative tolerance gene (TTG) clusters multiple onion-associated species similar to those characterized other Allium-associated endophytes pathogens. Sequence analysis revealed the presence three TTG cluster types, with both...

Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot onions in Georgia. Previous genomic studies identified two virulence gene clusters, HiVir alt, associated with rot. The cluster required to induce necrosis on onion tissues via synthesis pantaphos, (2-hydroxy[phosphono-methyl)maleate), phosphonate phytotoxin. alt aids tolerance thiosulfinates generated during tissue damage. Whole genome sequencing other species suggests that these clusters are present...

Pantoea stewartii subsp. indologenes is a causative agent of leafspot foxtail millet and pearl millet; however, novel strains were recently identified that are pathogenic on onion. We phenotypically genotypically characterized 17 P. from onion other sources (pearl millet, guar pulse, verbena, corn). Based the host range evaluation, we propose two pathovars: pv. cepacicola nov. setariae causes symptoms Allium spp. (leek, onion, chive, Japanese bunching onion) oat. However, can only infect...

Pantoea ananatis, a gram negative and facultative anaerobic bacterium is member of spp. complex that causes center rot onion, which significantly affects onion yield quality. This pathogen does not have typical virulence factors like type II or III secretion systems but appears to require biosynthetic gene-cluster, HiVir/PASVIL (located chromosomally comprised 14 genes), for phosphonate secondary metabolite, the ‘ alt ’ gene cluster in plasmid 11 genes) aids bacterial colonization bulbs by...

ABSTRACT Phage-derived bacteriocins are highly specific and effective antimicrobial molecules, which have successfully been used as prophylactic treatments to prevent phytopathogen infections. Given the specificity of tailocins, a necessary step for broadening tailocin catalog extending applicability across systems diseases is screening new clades phytopathogens production molecules with tailocin-like killing activity. Here, we describe by sensitivity strains tailocins produced Pantoea...

Onion center rot is caused by at least four species of genus Pantoea (P. ananatis, P. agglomerans, allii, and stewartii subsp. indologenes). Critical onion pathogenicity determinants for ananatis were recently described, but whether those are common among other onion-pathogenic remains unknown. In this work, we report in indologenes allii. We identified two distinct secondary metabolite biosynthetic gene clusters present separately different strains indologenes. One cluster similar to the...

Abstract Onion ( Allium. cepa L), garlic A. sativum L.), and other members of the Allium genus produce volatile antimicrobial thiosulfinates upon cellular damage. Allicin has been known since 1950s as primary thiosulfinate compound odorant produced by garlic. However, roles endogenous production in host-bacterial pathogen interactions have not described. The bacterial onion Pantoea ananatis , which lacks both virulence Type III II Secretion Systems, induces necrotic symptoms extensive cell...

Burkholderia gladioli pv. alliicola, B.cepacia, and B. orbicola are common bacterial pathogens of onion. Onions produce organosulfur thiosulfinate defensive compounds after cellular decompartmentalization. Using whole genome sequencing in silico analysis, we identified putative tolerance gene (TTG) clusters multiple onion-associated species similar to those characterized other Allium-associated endophytes pathogens. Sequence analysis revealed the presence three TTG cluster types with both...

Abstract Background : Pantoea ananatis is a member of spp. complex that causes center rot onion, which significantly affects onion yield and quality. This pathogen does not have typical virulence factors like type II or III secretion systems but appears to require biosynthetic gene-cluster, HiVir/PASVIL (located chromosomally), for phosphonate secondary metabolite, the onion-virulence regions, OVR (localized on megaplasmid), pathogenicity virulence, respectively. Results: We conducted deep...

Abstract Phage-derived bacteriocins are highly specific and effective antimicrobial molecules which have successfully been used as prophylactic treatments to prevent phytopathogen infections. Given the specificity of tailocins, a necessary step for broadening tailocin catalog extending applicability across systems diseases is screening new clades phytopathogens production with tailocin-like killing activity. Here, we describe by sensitivity strains tailocins produced Pantoea ananatis...

Abstract Pantoea ananatis is a member of spp. complex that causes center rot onion, which significantly affects onion yield and quality. This pathogen does not have typical virulence factors like type II or III secretion systems but appears to require biosynthetic gene-cluster, HiVir/PASVIL (located chromosomally), for phosphonate secondary metabolite, the onion-virulence regions, OVR (localized on megaplasmid), pathogenicity virulence, respectively. We conducted deep pan-genome-wide...

Pantoea ananatis is a member of species complex that causes center rot bulb onions (A. cepa) and also infects other Allium crops like leeks (Allium porrum), chives schoenoprasum), bunching onion or Welsh fistulosum), garlic sativum). This pathogen relies on chromosomal phosphonate biosynthetic gene cluster (HiVir) plasmid-borne thiosulfinate tolerance (alt) for pathogenicity virulence, respectively. However, virulence factors associated with remain unknown. We used phenotype-dependent...

Abstract Background The PathogenDx DetectX Combined method is a certified Performance Tested MethodSM (012201) that enrichment-free and utilizes DNA microarray-based end point PCR for the simultaneous detection of Aspergillus (A. flavus, A. fumigatus, niger, terreus), Salmonella spp., broad range Shiga toxin-producing Esherichia coli (STEC) from hemp cannabis flower, edibles, concentrates. Objective This study aimed to compare four AOAC INTERNATIONAL molecular methods utilize enrichment...

Abstract Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot onions in Georgia. Previous genomic studies identified two virulence gene clusters, HiVir alt , associated with rot. The cluster required to induce necrosis on onion tissues via synthesis predicted small molecule toxin. aids tolerance thiosulfinates generated during tissue damage. Whole genome sequencing other species suggest that these clusters are present outside P. . To assess distribution...

Abstract Onion center rot is caused by at least four species of Pantoea ( P. ananatis, agglomerans, allii, and stewartii subsp. indologenes ). Critical onion pathogenicity determinants for ananatis were recently described but whether those are common among other onion-pathogenic remains unknown. In this work, we report in allii . We identified two distinct secondary metabolite biosynthetic gene clusters different strains pathogenic One cluster similar to the previously HiVir phosphonate...