A5058421026- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Immune responses and vaccinations
- Respiratory viral infections research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Microbial Inactivation Methods
- Metabolism, Diabetes, and Cancer
- Molecular Biology Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Gene expression and cancer classification
- Nutrition and Health in Aging
University of Arizona
2020-2022
Dermatology Research Center
2021
Rationale: Cigarette smoke (CS) inhalation triggers oxidative stress and inflammation, leading to accelerated lung aging, apoptosis, emphysema, as well systemic pathologies. Metformin is beneficial for protecting against aging-related diseases. Objectives: We sought investigate whether metformin may ameliorate CS-induced pathologies of emphysematous chronic obstructive pulmonary disease (COPD). Methods: Mice were exposed chronically CS fed metformin-enriched chow the second half exposure....
Abstract Background How cigarette smoke (CS) and chronic obstructive pulmonary disease (COPD) affect severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection severity is controversial. We investigated the effects of COPD CS on expression SARS-CoV-2 entry receptor ACE2 in vivo patients controls CS-exposed mice, human bronchial epithelial cells vitro. Methods quantified: (1) protein levels by immunostaining ELISA, both and/or TMPRSS2 mRNA RT-qPCR two independent cohorts; (2) ELISA...
ABSTRACT Introduction How cigarette smoke (CS) and chronic obstructive pulmonary disease (COPD) affect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection severity is controversial. We investigated the protein mRNA expression of SARS-CoV-2 entry receptor ACE2 proteinase TMPRSS2 in lungs from COPD patients controls, lung tissue mice exposed acutely chronically to CS. Also, we effects CS exposure on human bronchial epithelial cells. Methods In Cohort 1, ACE2-positive cells...
People with pre-existing lung diseases such as chronic obstructive pulmonary disease (COPD) are more likely to get very sick from SARS-CoV-2 2019 (COVID-19). Still, an interrogation of the immune response COVID-19 infection, spatially throughout structure, is lacking in patients COPD. For this study, we characterized microenvironment parenchyma, airways, and vessels never- ever-smokers or without COPD, all whom died COVID-19, using spatial transcriptomic proteomic profiling. The COPD...
The PathogenDx family of assays uses microarray technology to simultaneously detect the presence bacterial and fungal pathogens in food products, environmental surfaces, cannabis products.The Detectx Combined assay was validated for detection Aspergillus, (Aspergillus flavus, Aspergillus fumigatus, niger, terreus), Salmonella, a broad range STEC (stx1 and/or 2) species. validation consisted two matrix studies dried hemp flower (>0.3% delta-9 tetrahydrocannabinol) flower, product consistency,...
Abstract Rationale People with pre-existing lung diseases like chronic obstructive pulmonary disease (COPD) are more likely to get very sick from SARS-CoV-2 2019 (COVID-19), but an interrogation of the immune response COVID-19 infection, spatial throughout structure is lacking in patients COPD. Objectives To profile microenvironment parenchyma, airways, and vessels never- ever-smokers or without COPD, whom all died COVID-19, using transcriptomic proteomic profiling. Findings The COPD...
Abstract Background The PathogenDx DetectX Combined method is a certified Performance Tested MethodSM (012201) that enrichment-free and utilizes DNA microarray-based end point PCR for the simultaneous detection of Aspergillus (A. flavus, A. fumigatus, niger, terreus), Salmonella spp., broad range Shiga toxin-producing Esherichia coli (STEC) from hemp cannabis flower, edibles, concentrates. Objective This study aimed to compare four AOAC INTERNATIONAL molecular methods utilize enrichment...