A5046812792- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Mass Spectrometry Techniques and Applications
- Neuroscience and Neural Engineering
University of Vienna
2023
Abstract Inwardly rectifying potassium (Kir) channels open at the ‘helix bundle crossing’ (HBC), formed by M2 helices cytoplasmic end of transmembrane pore. Introduced negative charges HBC (G178D) in Kir2.2 forces opening, allowing pore wetting and free movement permeant ions between cytoplasm inner cavity. Single-channel recordings reveal striking, pH-dependent, subconductance behaviors G178D (or G178E equivalent Kir2.1[G177E]) mutant channels, with well-resolved non-cooperative levels....
Inwardly rectifying potassium (Kir) channels play a critical role in stabilizing the membrane potential, thus controlling numerous physiological phenomena multiple tissues. Channel conductance is activated by cytoplasmic modulators that open channel at 'helix bundle crossing' (HBC), formed coming together of M2 helices from each four subunits, end transmembrane pore. We introduced negative charge crossing region (G178D) classical inward rectifier Kir2.2 subunits forces opening, allowing pore...