The protist Plasmodiophora brassicae is a biotrophic soil-borne pathogen that causes clubroot on Brassica crops worldwide. Clubroot disease serious threat to the 8 M ha of canola (Brassica napus) grown annually in western Canada. While host resistance key management, sources are limited. To identify new (CR), we fine mapped CR gene (Rcr1) from B. rapa ssp. chinensis region between 24.26 Mb and 24.50 linkage group A03, with several closely linked markers identified. Transcriptome analysis was...

Clubroot disease caused by Plasmodiophora brassicae Woronin is a concern to the canola (Brassica napus L.) growers in Canada. A crop management strategy that includes deployment of genetic resistance and appropriate cultural practices needed for long-term this disease. Resistance P. pathotypes present Canada has been found primary secondary gene pools spring B. canola. Some these sources, such as winter 'Mendel', rutabaga Pak Choi rapa 'Flower Nabana', were used studies breeding development...

This study investigated how the timing of application biofungicide Serenade (Bacillus subtilis QST713) or it components (product filtrate and bacterial cell suspension) influenced infection canola by Plasmodiophora brassicae under controlled conditions. The its were applied as a soil drench at 5% concentration (vol/vol equivalent CFU) to planting mix infested with P. seeding transplanting 7 14 days after (DAS) target primary secondary zoospores brassicae. Quantitative polymerase chain...

Abstract Clubroot, caused by Plasmodiophora brassicae , is an important disease of Brassica crops worldwide. F 1 progeny from the rapa lines T19 (resistant) × ACDC (susceptible) were backcrossed with ACDC, then self-pollinated to produce BC S lines, From genotyping-by-sequencing (GBS) parental and plants, about 1.32 M sequences aligned into reference genome B . 0.4-fold coverage, 1.77 0.5-fold coverage in ACDC. The number short reads per plant ranged 0.07 1.41 0.1-fold coverage. A total 1584...

Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified fine mapped Chinese cabbage cv. 'Jazz' using SNP markers from bulked segregant RNA sequencing (BSR-Seq) molecular were developed for use marker assisted selection. total, 203.9 million raw reads generated one pooled resistant (R) susceptible (S) sample, >173,000 polymorphic single nucleotide...

Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. was previously mapped to chromosome A03 B. rapa in pak choy cultivar "Flower Nabana". In the current study, pathotypes 2, 5 and 6 shown be associated Rcr1 region A03. Bulked segregant RNA sequencing performed short read sequences were assembled into 10 chromosomes reference genome v1.5. For resistant (R) bulks, a total...

Resistance to the blackleg disease of Brassica napus (canola/oilseed rape), caused by hemibiotrophic fungal pathogen Leptosphaeria maculans, is determined both race-specific resistance (R) genes and quantitative loci (QTL), or adult-plant (APR). While introgression R into breeding material relatively simple, QTL are often detected sporadically, making them harder capture in programs. For effective deployment APR crop varieties, need have a reliable influence on phenotype multiple...

Seven blackleg resistance (R) genes (Rlm1, Rlm2, Rlm3, Rlm4, LepR1, LepR2 & LepR3) were each introgressed into a common susceptible B. napus doubled-haploid (DH) line through reciprocal back-crossing, producing single-R gene introgression lines (ILs) for use in the pathological and molecular study of Brassica – Leptosphaeria interactions. The genomic positions R defined mapping analysis with transgenic L. maculans isolates was used to confirm identity where possible. Using contrasting...

Genetic resistance is widely used to manage clubroot (Plasmodiophora brassicae) in brassica crops, but new pathotypes have recently been identified on canola (Brassica napus) the Canadian prairies. Resistance effective against both most prevalent pathotype (3H, based Clubroot Differential system) and needed. BC1 plants of Brassica rapa from a cross line 96-6990-2 (clubroot originating turnip cultivar ‘Waaslander’) susceptible doubled-haploid line, ACDC, exhibited 1:1 segregation for 3H 5X. A...

The spectrum of resistance to isolates Leptosphaeria maculans and the map location a new blackleg gene found in canola cultivar Brassica napus ‘Surpass 400’ are described. Two genes, LepR1 LepR2, from B. rapa subsp. sylvestris introgressed were identified previously. also has sylvestris. Using 31 diverse L. maculans, disease reaction was compared with those resistant breeding lines AD9 (which contains LepR1), AD49 LepR2), MC1-8 both LepR2). on different observed MC1-8, indicating that...

Abstract Two cabbage ( Brassica oleracea ) cultivars ‘Tekila’ and ‘Kilaherb’ were identified as resistant to several pathotypes of Plasmodiophora brassicae . In this study, we a clubroot resistance gene Rcr7 in for pathotype 3 P. from segregating population derived crossed with the susceptible line T010000DH3. Genetic mapping was performed by identifying percentage polymorphic variants (PPV), new method proposed through bulked segregant RNA sequencing. Chromosome C7 carried highest PPV (42%)...

Clubroot, caused by Plasmodiophora brassicae Woronin, is a very important disease of Brassica species. Management clubroot relies heavily on genetic resistance. In cross nigra lines PI 219576 (highly resistant, R) × CR2748 susceptible, S) to clubroot, all F1 plants were resistant clubroot. There was 1:1 ratio R:S in the BC1 and 3R:1S F2, which indicated that single dominant gene controlled resistance 219576. This designated Rcr6. Mapping Rcr6 performed using genome sequencing information...

Plasmodiophora brassicae causes clubroot disease in brassica crops worldwide. Brassica rapa, a progenitor of napus (canola), possesses important sources for resistance to clubroot. A doubled haploid (DH) population consisting 84 DH lines were developed from Backcross2 (BC2) plant through an interspecific cross B. rapa turnip cv. ECD01 (resistant, R) with canola line DH16516 (susceptible, S) and then backcrossed as the recurrent parent. The their parental tested four major pathotypes (3A, 3D,...

A collection of 955 Brassica accessions including B. rapa (718), napus (94), juncea (93), oleracea (30), carinata (12) and nigra (8) was screened against Plasmodiophora brassicae pathotype 3 (1 × 106 resting spores cc−1 growth medium), the predominant strain pathogen on canola in western Canada. total 35 (mostly rapa) showed at least 50% reduced clubroot severity relative to a susceptible control, with 15 showing complete resistance (clubroot-free). Ten resistant representing A-, B- C-genome...

The protection of canola (Brassica napus) crops against blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is largely mediated race-specific resistance genes (R-genes). While many R-genes effective disease have been identified in Brassica species, information precise genomic locations limited. In this study, Rlm2 gene for to blackleg, located on chromosome A10 B. napus cultivar 'Glacier', was targeted fine mapping. Molecular markers tightly linked were developed use...

Clubroot is an important disease of brassica crops world-wide. The causal agent, Plasmodiophora brassicae, has been present in Canada for over a century but was first identified on canola (Brassica napus) Alberta, 2003. Genetic resistance to clubroot adapted cultivar available since 2009, breakdown detected 2013 and new pathotypes are increasing rapidly. Information genetic similarity among pathogen populations across could be useful estimating the variation populations, predicting effect...

Clubroot disease is a serious threat to canola production in western Canada and many parts of the world. Rcr1 clubroot resistance (CR) gene identified recently its molecular mechanisms mediating CR have been studied using several omics approaches. The current study aimed characterize biochemical changes cell wall roots connecting key this prior studies Fourier transform infrared (FTIR) spectroscopy. expression nine genes involved phenylpropanoid metabolism was also qPCR. Between susceptible...

Clubroot, caused by the plasmodiophorid pathogen Plasmodiophora brassicae, is one of most serious diseases on Brassica crops worldwide and a major threat to canola production in western Canada. Host resistance key strategy for clubroot management canola. Several (CR) genes have been identified, but mechanisms associated with these CR are poorly understood. In current study, label-free shotgun proteomic approach was used profile compare proteomes B. rapa carrying not gene Rcr1 upon P....

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out two sets extreme pools, group I and II, which were constructed separately from 110 74 F2 cloned lines derived the cross between clubroot-resistant (R) "GZ87" (against race 4) susceptible (S) "263." Based on QTL-sequencing (QTL-Seq) analysis three QTLs (i.e., qCRc7-2,...

Abstract Breeding for disease resistance in major crops is of crucial importance global food security and sustainability. However, common biotechnologies such as traditional transgenesis or genome editing do not provide an ideal solution, whereas transgenic free selection markers cisgenic/intragenic might be suitable. In this study, after cloning functional verification the Rcr1 gene to clubroot (Plasmodiophora brassicae), we confirmed that genes Rcr1, Rcr2, Rcr4, CRa from Brassica rapa B....