Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular trafficking have been shown to generate tubules. The degree bending appears be encoded structure immanent curvature particular protein domains, with BAR F-BAR domains inducing high- low-curvature tubules, respectively. In addition, oligomerization formation ordered arrays influences tubule stabilization. Here, domain-containing Pacsin was found possess a unique...

The protist Plasmodiophora brassicae is a biotrophic soil-borne pathogen that causes clubroot on Brassica crops worldwide. Clubroot disease serious threat to the 8 M ha of canola (Brassica napus) grown annually in western Canada. While host resistance key management, sources are limited. To identify new (CR), we fine mapped CR gene (Rcr1) from B. rapa ssp. chinensis region between 24.26 Mb and 24.50 linkage group A03, with several closely linked markers identified. Transcriptome analysis was...

Clubroot, caused by Plasmodiophora brassicae Wor., is an important soilborne disease of canola (Brassica napus L.) in Canada that managed mainly planting clubroot-resistant (CR) cultivars. Populations P. representing 106 fields Alberta were obtained from galled roots CR plants collected 2014–2016 and characterized for virulence on seven Sixty-one these populations could overcome resistance at least one cultivar evaluated further inoculation 13 Brassica hosts termed the Canadian Clubroot...

AbstractSelect biofungicides and fungicides, used alone or with cultivar resistance crop rotation, were assessed for their potential in integrated management of clubroot disease. The synthetic fungicides pentachloronitrobenzene, fluazinam cyazofamid showed activities against Plasmodiophora brassicae. Serenade® Prestop® also suppressed the disease on canola via antibiosis induced host under controlled-environment conditions. Granular seed-treatment formulations developed to facilitate...

Clubroot disease caused by Plasmodiophora brassicae Woronin is a concern to the canola (Brassica napus L.) growers in Canada. A crop management strategy that includes deployment of genetic resistance and appropriate cultural practices needed for long-term this disease. Resistance P. pathotypes present Canada has been found primary secondary gene pools spring B. canola. Some these sources, such as winter 'Mendel', rutabaga Pak Choi rapa 'Flower Nabana', were used studies breeding development...

This study investigated how the timing of application biofungicide Serenade (Bacillus subtilis QST713) or it components (product filtrate and bacterial cell suspension) influenced infection canola by Plasmodiophora brassicae under controlled conditions. The its were applied as a soil drench at 5% concentration (vol/vol equivalent CFU) to planting mix infested with P. seeding transplanting 7 14 days after (DAS) target primary secondary zoospores brassicae. Quantitative polymerase chain...

Abstract Clubroot, caused by Plasmodiophora brassicae , is an important disease of Brassica crops worldwide. F 1 progeny from the rapa lines T19 (resistant) × ACDC (susceptible) were backcrossed with ACDC, then self-pollinated to produce BC S lines, From genotyping-by-sequencing (GBS) parental and plants, about 1.32 M sequences aligned into reference genome B . 0.4-fold coverage, 1.77 0.5-fold coverage in ACDC. The number short reads per plant ranged 0.07 1.41 0.1-fold coverage. A total 1584...

Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified fine mapped Chinese cabbage cv. 'Jazz' using SNP markers from bulked segregant RNA sequencing (BSR-Seq) molecular were developed for use marker assisted selection. total, 203.9 million raw reads generated one pooled resistant (R) susceptible (S) sample, >173,000 polymorphic single nucleotide...

Importance Paroxysmal supraventricular tachycardia (PSVT), defined as tachyarrhythmias that originate from or conduct through the atria atrioventricular node with abrupt onset, affects 168 to 332 per 100 000 individuals. Untreated PSVT is associated adverse outcomes including high symptom burden and tachycardia-mediated cardiomyopathy. Observations Approximately 50% of patients are aged 45 64 years 67.5% female. Most common symptoms include palpitations (86%), chest discomfort (47%), dyspnea...

Abstract Mycelial fungi, yeasts, and bacteria were isolated from strawberry plants evaluated for biocontrol of Botrytis cinerea in strawberry. Comparative tests done on leaf discs detached petals the laboratory attached leaves flowers growth room, greenhouse, field plots. In various controlled environments, tissues inoculated with microorganisms (107 fungal spores or yeast cells, 108 bacterial cfu/mL) challenge-inoculated pathogen (106 spores/mL) after 24 h. After a further h, transferred to...

(1992). Effectiveness of Honey Bees for Applying the Biocontrol Agent Gliocladium Roseum to Strawberry Flowers Suppress Botrytis Cinerea. Canadian Journal Plant Pathology: Vol. 14, No. 2, pp. 117-129.

The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening novel pathogenic isolates on Arabidopsis, we found significant differences responses between detached and attached leaf assays. A near-adapted isolate linicola A1 could launch a typical infection only detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated intact plants also was compromised leaves...

Clubroot caused by Plasmodiophora brassicae is an emerging threat to canola ( Brassica napus ) production in western Canada, and a serious disease on crucifer vegetable crops eastern Canada. In this study, seven biological control agents two fungicides were evaluated as soil drenches or seed treatments for of clubroot. Under growth cabinet conditions, soil‐drench application formulated biocontrol Bacillus subtilis Gliocladium catenulatum reduced clubroot severity more than 80% relative...

Clubroot, caused by Plasmodiophora brassicae, has emerged as a serious disease threatening the canola production industry in western Canada. This review summarizes results from studies, conducted since 2007, on development of effective strategies for management clubroot canola. Several options have been proposed control this infested fields, including liming soil to increase pH, crop rotation with non-hosts and bait crops, manipulating sowing date, sanitization farm equipment, deployment...

An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated roots and colonized cortical tissues. Based qPCR analysis, the amount of DNA found at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with severity 5 weeks a low (2×105 spores pot−1) but not high dose pathogen inoculum. Transcript levels nine B. napus (Bn) genes...

The impact of cultivar resistance and inoculum density on the incidence primary infection canola root hairs by Plasmodiophora brassicae , causal agent clubroot, was assessed microscopy. hair in both a resistant susceptible increased with increasing density, but two‐ to threefold higher cultivar; relationship between also substantially stronger more consistent cultivar. In cultivar, rate peaked 6 8 days after sowing then declined. it over 14‐day duration each study. It appears that...

Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. was previously mapped to chromosome A03 B. rapa in pak choy cultivar "Flower Nabana". In the current study, pathotypes 2, 5 and 6 shown be associated Rcr1 region A03. Bulked segregant RNA sequencing performed short read sequences were assembled into 10 chromosomes reference genome v1.5. For resistant (R) bulks, a total...

Different phosphoinositides enriched at the membranes of specific subcellular compartments within plant cells contribute to organelle identity, ensuring appropriate cellular trafficking and function. During infection cells, biotrophic pathogens such as powdery mildews enter differentiate into haustoria. Each haustorium is enveloped by an extrahaustorial membrane (EHM) derived from host plasma membrane. Little known about EHM biogenesis identity. Here, we demonstrate that among two in Arabidopsis (

Genetic resistance is widely used to manage clubroot (Plasmodiophora brassicae) in brassica crops, but new pathotypes have recently been identified on canola (Brassica napus) the Canadian prairies. Resistance effective against both most prevalent pathotype (3H, based Clubroot Differential system) and needed. BC1 plants of Brassica rapa from a cross line 96-6990-2 (clubroot originating turnip cultivar ‘Waaslander’) susceptible doubled-haploid line, ACDC, exhibited 1:1 segregation for 3H 5X. A...

SUMMARY Clubroot, caused by Plasmodiophora brassicae , is one of the most serious diseases cultivated cruciferous crops in world. However, basis for pathogenicity P. not well understood. In this study, a serine protease gene ( PRO1 ) was cloned from and its molecular characteristics were investigated. Southern analysis specific polymerase chain reaction (PCR) amplification indicated that single‐copy present broad range pathotypes. Northern revealed expression induced during plant infection,...

Hwang, S. F., Ahmed, H. U., Strelkov, E., Gossen, B. D., Turnbull, G. Peng, and Howard, R. J. 2011. Seedling age inoculum density affect clubroot severity seed yield in canola. Can. Plant Sci. 91: 183–190. Clubroot, caused by Plasmodiophora brassicae, is a serious threat to canola (Brassica napus, rapa) production western Canada because of its long-lived resting spores, high spore potential, negative impact on when pressure high. The effect was studied diluting heavily infested field soil...