Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use domestic cats, are used some cheetah populations have been anecdotally linked to disease certain subpopulations. Ten FHV-1 isolates from ten one isolate an MLV inoculate four of the host animals were analyzed. Viral DNA was extracted full-genome sequencing by Illumina MiSeq with viral genomes then phylogenomic recombinational analyses....

ABSTRACT A 6‐year‐old castrated male mixed breed dog presented to the “Louisiana State University Veterinary Teaching Hospital &‐anonymized for review” ophthalmology service with marked exophthalmos and lagophthalmos of left eye soft tissue swelling medial zygomatic arch. computed tomography (CT) scan was performed, it revealed a well‐marginated, lobulated, homogenously fat density involving retrobulbar space. Preliminary results fine‐needle aspirate (FNA) biopsy were not conclusive,...

Keratomycosis is rarely reported in dogs. The purpose of this study was to review the signalment, clinical characteristics, predisposing factors, and outcome 11 cases canine keratomycosis. Medical records included dogs were reviewed follow-up information obtained by re-examination patients following their initial diagnosis. All possessed factors for fungal keratitis, including an underlying endocrinopathy, pre-existing corneal disease, intraocular surgery, and/or prolonged use either topical...

Herpes simplex virus type-1 (HSV-1) can cause severe ocular infection and blindness. We have previously shown that the HSV-1 VC2 vaccine strain is protective in mice guinea pigs against genital herpes following vaginal challenge with or HSV-2. In this study, we evaluated efficacy of intramuscular vaccination herpetic keratitis lethal human clinical HSV-1(McKrae). produced superior protection morbidity control comparison to its parental HSV-1(F). Specifically, after HSV-1(McKrae) challenge,...

Abstract Polymeric nanoparticles have been investigated as potential delivery systems for therapeutic compounds to address many ailments including eye disease. The stability and spatiotemporal distribution of polymeric in the are important regarding practical applicability efficacy system treating We selected poly(lactic-co-glycolic acid) (PLGA) loaded with lutein, a carotenoid antioxidant associated health, our model ophthalmic nanodelivery evaluated its when suspended various conditions...

Abstract Case Description —A great horned owl of estimated age < 1 year that was captured by wildlife rehabilitators evaluated because suspected cataracts. Clinical Findings —Nuclear and incomplete cortical cataracts were evident in both eyes. Ocular ultrasonography revealed no evidence retinal detachment, electroretinography normal function. Treatment Outcome —For visual rehabilitation, cataract surgery planned intraocular lens design determined on the basis values obtained from...

Antioxidant therapies are of interest in the prevention and management ocular disorders such as cataracts. Although an active area interest, topical therapy with antioxidants for treatment cataracts is complicated by multiple anatomical barriers, product stability, solubility. Entrapment delivery poly(lactic- co -glycolic acid) nanoparticles a possible solution to these challenges, however, little known regarding their effects vitro or vivo. Our first aim was investigate impact blank lutein...

Purpose: To determine matrix metalloproteinase (MMP) 2 and MMP 9 expression in acute chronic experimentally wounded canine corneas keratectomy samples from patients with spontaneous corneal epithelial defects (SCCEDs). Methods: Mechanical debridement was performed unilaterally 25 healthy dogs for the wound study. Twenty-four hours (n = 8), 48 5), 72 3), or 1 week 9) after wounding, were euthanized. Debridement once weekly 8 weeks study 8). Therapeutic superficial keratectomies 16) on SCCED...

Dogs diagnosed with sudden acquired retinal degeneration syndrome (SARDS) commonly are presented concurrent clinical, physical, and historical findings consistent hyperadreno-corticism (HAC) at the time of vision loss. Thirteen dogs SARDS on basis complete ophthalmic examination extinguished bright-flash electroretinogram were evaluated for steroid hormonal abnormalities. Signalment, case history, physical examination, clinicopathological recorded. Serum cortisol sex-hormone concentrations...

Black soldier fly (BSF) larvae have been marketed as an excellent choice for providing calcium to reptiles without the need of dusting or gut loading. However, previous studies indicated that they limited digestibility and are deficient in fat soluble vitamins (A, D3, E). In this feeding trial, 24 adult male leopard geckos were fed one three diets 4 months: 1) whole, vitamin A loaded larvae; 2) needle pierced, 3) non-gut larvae. Fecal output from was collected daily apparent calculated dry...

Background: Family Medicine Groups (FMG) were introduced in Quebec 2002 to re-organize primary care practices and encourage inter-professional service delivery.We measured visits the emergency department (ED) for acute complications related diabetes as a proxy access quality of care, before after reform using an open cohort individuals diagnosed with type 1 2 diabetes.Methods: The weekly rate ED between April 1, 2000 March 31, 2012 derived from administrative databases.We performed...

Heat-shock proteins, particularly the 70-kDa member (Hsp70), have been implicated in facilitating wound healing multiple tissues. Expression and localization of three HSPs were assessed normal wounded canine corneas to elucidate a role epithelial healing.Paraffin-embedded corneas, acute repeatedly abraded keratectomies spontaneous chronic corneal defects (SCCEDs) subjected routine immunohistochemistry for Hsp27, 47, 70 expression. Ex vivo created treated with anti-HSPs or IgG controls, was...

Abstract Objective To assess the efficacy of compounded cidofovir, famciclovir, and ganciclovir for treatment feline herpesvirus type 1 (FHV‐1) ocular surface disease. Animals studied 132 shelter‐housed cats qPCR positive FHV‐1. Procedures A masked placebo‐controlled study design was utilized. were enrolled in one four groups: topical placebo + oral ( n = 32), cidofovir 0.5% ophthalmic solution famciclovir (90 mg/kg) 0.15% 36). Cats treated with each medication twice daily 7 days evaluated...

Abstract Objective To investigate the effects of environmental temperature and relative humidity on tear test results in dogs. Animals studied 22 normal Procedures Routine Schirmer tests (STT1) phenol red thread (PRTT) were administered to dogs four conditions with different (RH) (T). Environmental (mean ± SD) included following: indoor (RH = 49 4%, T 72 2°F), outdoor 62 11%, 85 6°F), high 6%, 71 1°F), low 39 2%, 76 1°F). ANCOVA models used assess for significant associations between...

Abstract Objective To determine whether wetting length of Schirmer tear test (STT) strips varies by commercial manufacturer test. Animals Studied Ten normal female beagle cross‐breed dogs. Procedures from four manufacturers were used to assess lengths in‐vitro when exposed a set volume water over 1 minute. Digital photography was the total surface area each manufacturer. type performed on dogs minute with STT same in randomized crossover design. ANOVA detect differences between different...

Feline herpesvirus type 1 (FHV-1) commonly causes ocular surface disease in cats and is treated with antiviral medications targeting viral DNA polymerase (UL30/42). Herein, we describe a method to assess the FHV-1 genome for mutation development functional impact of mutations, if present. Fourteen shelter-housed domestic were assigned one four treatment groups: placebo ( n = 3), cidofovir 0.5% ophthalmic solution famciclovir oral 5), or ganciclovir 0.15% 3). Swabs collected before (day 1)...

Abstract Objective To assess intraocular pressure (IOP) development in cranes and determine the impact of age, weight, species, head position, sex. Animals Studied Whooping (WC) ( Grus americana ), Mississippi‐sandhill (MSC) canadensis pulla ). Procedures Chicks were manually restrained on days 1–3, 7, 21, 35, 60, 75, 120 for routine examinations. IOP was opportunistically measured utilizing Tonovet Plus® D setting with above heart (AH) below (BH). Values also obtained longitudinally adults...

It is currently unknown which of the two devices most commonly used in equine ophthalmology for intraocular pressure (IOP) estimation demonstrates lowest inter-user and intra-user variation.To assess variation tonometers sedated unsedated horses.Randomised masked cross-over trial.Four examiners rebound (ICare® TonoVet) applanation (TonoPen® ) to measure triplicate 10 normal horses before after sedation with xylazine. For variation, coefficient (CV) values were calculated from mean each...

Objectives Feline herpesvirus-1 (FHV-1) is a prevalent cause of ocular disease in cats and limited topical options for treatment currently exist. The first objective this study was to confirm the efficacy ganciclovir against FHV-1 vitro. second assess safety tolerability topically applied eye gel (GEG) healthy cats. Methods used infect tissue culture wells covered maximally confluent Crandall–Rees feline kidney cells prior addition three molarities (8.9 µM, 17.8 µM 89 µM) before being...