A5091720329- Helicobacter pylori-related gastroenterology studies
- Oral microbiology and periodontitis research
- Veterinary medicine and infectious diseases
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Eosinophilic Esophagitis
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Gastrointestinal motility and disorders
- Salivary Gland Disorders and Functions
- Bacterial Genetics and Biotechnology
- Legionella and Acanthamoeba research
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Antimicrobial Peptides and Activities
- Galectins and Cancer Biology
- Gastroesophageal reflux and treatments
- Bacterial Infections and Vaccines
- Gastrointestinal disorders and treatments
- Infective Endocarditis Diagnosis and Management
- RNA and protein synthesis mechanisms
- Pharmacological Effects of Natural Compounds
- Antimicrobial Resistance in Staphylococcus
Tokyo Dental College
2001-2025
Kobe Pharmaceutical University
2024
Kyorin University
2013-2022
National Institute of Infectious Diseases
2006-2017
Yokohama City University
2016
Nihon University
2009
Tokyo Medical and Dental University
2009
University at Buffalo, State University of New York
2005
Juntendo University
2004
Helicobacter pylori forms biofilms on glass surfaces at the air-liquid interface in vitro batch cultures; however, of H. have not been well characterized. In present study, we analyzed ability strains to form and characterized underlying mechanisms biofilm formation. Strain TK1402 showed strong forming relative other Brucella broth supplemented with 7% FCS. The is reflected thickness biofilms. addition, outer membrane vesicles (OMV) were detected within matrix only Biofilm formation was...
In contrast to most modern pharmaceuticals, probiotics are used in many parts of the world with little or no research data on complex system interactions that each strain may elicit human body. Research has recently become more significant, as have begun be prescribed by clinicians an alternative for some gut infections, especially when antibiotics contraindicated. This study attempted elucidate inhibitory interaction between Japanese probiotic Clostridium butyricum MIYAIRI 588 (CBM588) and...
The human gastric pathogen Helicobacter pylori forms biofilms in vitro and vivo. purpose of this study was to evaluate the effects H. biofilm formation on clarithromycin (CLR) susceptibility. CLR susceptibility intermediate (2-day) mature (3-day) glass coverslips determined at concentrations from 0.03 0.5 µg/ml. biomass increased after treatment with minimum inhibitory concentration levels by up 4-fold (2-day biofilm) 16-fold (3-day biofilm). Minimum bactericidal against cells a were higher...
A dipeptide lantibiotic, named Smb, in Streptococcus mutans GS5 was characterized by molecular genetic approaches. The Smb biosynthesis gene locus is encoded a 9.5-kb region of chromosomal DNA and consists seven genes the order smbM1, -T, -F, -M2, -G, -A, -B. This operon not present some other strains S. mutans, including strain UA159. encoding were identified as smbA smbB. Inactivation smbA, or smbB attenuated inhibition growth indicator RP66, confirming an essential role for these...
The effects of Streptococcus salivarius on the competence-stimulating peptide (CSP)-dependent biofilm formation by mutans were investigated.Biofilms grown 96-well microtiter plates coated with salivary components in tryptic soy broth without dextrose supplemented 0.25% sucrose. Biofilm formations stained using safranin and quantification biofilms was performed measuring absorbance at 492 nm.S. formed substantial biofilms, whereas S. poorly medium conditions used. Furthermore, combination...
Abstract Background Infection of H elicobacter pylori mainly occurs in childhood. In J apan, incidence gastric cancer is still high the senior citizen population, but little known about current . infection status among children or their family members. Methods As a population‐based study, prevalence and change over 1‐year interval were determined. Family members some participants also invited to participate study determine status. All specific ages attending 16 schools S asayama, yogo P...
Intra-familial infection is considered to be one of the main routes transmission for Helicobacter pylori in Japan. We assessed genomic profiles H. isolates from family members by multi-locus sequence typing (MLST) and identified original strain infecting index child. A total 19 five families were analysed MLST using seven housekeeping genes random amplification polymorphic DNA (RAPD)-PCR. Phylogenetic analysis was performed nucleotide sequences loci. Two or more different types strains...
We evaluated biofilm formation of clinical Helicobacter pylori isolates from Indonesia and its relation to antibiotic resistance. determined the minimum inhibition concentration (MIC) amoxicillin, clarithromycin, levofloxacin, metronidazole tetracycline by Etest measure planktonic susceptibility 101 H. strains. Biofilms were quantified crystal violet method. The eradication (MBEC) was obtained measuring survival bacteria in a after exposure antibiotics. majority strains formed (93.1%...
The epithelial cell rests of Malassez (ERM) are located within the periodontal ligament. They reportedly involved in maintaining homeostasis, particularly with regards to thickness Their role apical periodontitis lesions remains unclear, however. This study investigated response ERM infection Porphyromonas gingivalis. After being infected, morphology P. gingivalis-infected cells was observed using confocal laser-scanning microscopy. gene expression and uninfected by RNA-sequencing analysis....
Treponema denticola is frequently isolated together with Porphyromonas gingivalis from the lesions seen in cases of chronic periodontitis and considered a major pathogen this disease. It has several virulence factors, including surface protein (Msp) protease, dentilisin. The effect these factors on host immune response remains to be elucidated, however. Toll-like receptors (TLRs) can recognize pathogen-associated molecular patterns. Bacteria stimulate TLRs activate pro-inflammatory nuclear...
Abstract Background and Aims: Biofilms are surface‐bound communities of bacterial cells that implicated in their survival. As with various bacteria studied to date, Helicobacter pylori can have an alternate lifestyle as a biofilm. We previously reported strain TK1402 showed strong biofilm‐forming ability vitro . However, the mechanisms its biofilm development remain unclear. analyzed basic characteristics TK1402. Methods: In order characterize H. strains, auto‐aggregation, motility...
Bordetella pertussis, the causative agent of whooping cough, is highly adapted to cause human infection. The production virulence factors, such as adhesins and toxins, just part an array mechanisms by which B. pertussis causes stringent response a global bacterial nutritional limitation that mediated accumulation cellular ppGpp pppGpp [termed together (p)ppGpp]. Here, we demonstrate (p)ppGpp was controlled RelA SpoT proteins in mutation-induced loss both caused deficiencies production....
Mycoplasma pneumoniae is one of the causative organisms community-acquired pneumonia which found commonly in younger patients. Extrapulmonary complications similar to autoimmune disease are caused by M. following initial infection. The mechanism and pathology onset not clear, but it considered that excessive host immunoreactions play a part mycoplasmal its extrapulmonary complications. In this study, we investigated participation immune response, excluding Th1 Th2 has previously been...
Streptococcus mutans, the major pathogen responsible for dental caries in humans, is a biofilm-forming bacterium. In present study, 17 different pulsed-field gel electrophoresis patterns of genomic DNA were identified S. mutans organisms isolated clinically from whole saliva. The isolates showed abilities to form biofilms on polystyrene surfaces semidefined minimal medium cultures. Following cultivation flow cell system tryptic soy broth with 0.25% sucrose and staining using BacLight...
Helicobacter pylori is one of the most common causes bacterial infection in humans, and it forms biofilms on human gastric mucosal epithelium as well vitro abiotic surfaces. Bacterial biofilm critical not only for environmental survival but also successful infection. We previously demonstrated that strain TK1402, which was isolated from a Japanese patient with duodenal ulcers, has high biofilm-forming ability relative to other strains. In addition, we showed outer membrane vesicles (OMV)...
Helicobacter pylori, a bacterial pathogen that can infect human stomach causing gastritis, ulcers and cancer, is known to have high degree of genome/epigenome diversity as the result mutation recombination. The bacteria often in childhood persist for life host. One reasons rapid evolution H. pylori it changes its genome drastically adaptation new To investigate microevolution genome, we undertook whole sequencing same or very similar sequence type multi-locus typing (MLST) with seven genes...
This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori analyse intra-familial transmission. Faecal was and amplified by nested PCR. The products were analysed direct sequencing the allele type determined using an MLST website. Mother-to-child transmission suspected in at least two three families, father-to-child one family.
The antisense RNA ArrS is complementary to a sequence in the 5′ untranslated region of gadE T3 mRNA, largest transcript , which encodes transcriptional activator glutamate-dependent acid resistance system Escherichia coli . Expression arrS strongly induced during stationary growth phase, particularly under acidic conditions, and transcription dependent on σ S GadE. aim present study was clarify role controlling expression by overexpressing E. results showed marked increase survival...
Helicobacter pylori infection is a well-established risk factor for gastric cancer and has been linked to other gastrointestinal diseases, including pancreatic biliary tract cancers; however, the relevance of enterohepatic non-H. helicobacters pathophysiology these diseases remains unclear.We estimated prevalence two (Helicobacter hepaticus bilis) in framework hospital-based case-control study involving 121 patients with cancer, or diseases. Bile blood samples were collected from undergoing...
ABSTRACT Arginine-specific cysteine proteinases (RgpA and RgpB) produced by the periodontal pathogen Porphyromonas gingivalis are suspected virulence factors involved in interrupting host defense mechanisms as well penetrating destroying connective tissues. To induce a protective immune response against P. , we constructed an rgpA DNA vaccine. BALB/c mice were immunized intradermally Gene Gun with plasmid carrying . Antibody responses determined enzyme-linked immunosorbent assay. The vaccine...