A5114549543- Helicobacter pylori-related gastroenterology studies
- Mycobacterium research and diagnosis
- Veterinary medicine and infectious diseases
- Rabies epidemiology and control
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Gastric Cancer Management and Outcomes
- Galectins and Cancer Biology
- Eosinophilic Esophagitis
- Exercise and Physiological Responses
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Endometriosis Research and Treatment
- Respiratory viral infections research
- Food Allergy and Anaphylaxis Research
- Probiotics and Fermented Foods
- Uterine Myomas and Treatments
- Poxvirus research and outbreaks
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Gastrointestinal disorders and treatments
- Ovarian cancer diagnosis and treatment
- Animal Virus Infections Studies
Oita University
2016-2025
Kyushu University
2023-2024
Shonan Kamakura General Hospital
2023-2024
Ehime University Hospital
2024
Ehime University
2021-2024
Kinki Central Hospital
2024
Food Research Institute
2023
Kumamoto University
2022
Japanese Foundation For Cancer Research
2022
Osaka Hospital
2007-2021
A filamentous fungus, Magnaporthe oryzae, is a causal agent of rice blast disease, which one the most serious diseases affecting cultivated rice, Oryza sativa. However, molecular mechanisms underlying both defense and fungal attack are not yet fully understood. Extensive past studies have characterized many infection-responsive genes in pathogen host plant, separately. To understand plant-pathogen interaction comprehensively, it valuable to monitor gene expression profiles interacting...
Incidence and mortality of gastric cancer (GC) are high in Mongolia despite Helicobacter pylori the Mongolian population being less virulent.To evaluate bacterial microbiota profiles patients with GC its precursor histological conditions.We conducted a case-control study among 48 120 noncancer (20 normal mucosa [control], 20 gastritis, 40 atrophy intestinal metaplasia [IM]). We performed 16S rRNA gene amplicon sequencing compared taxonomic functional prediction based on diagnosis group H...
We evaluated the primary resistance of Helicobacter pylori (H. pylori) to routinely used antibiotics in Cambodia, an unexplored topic country, and assessed next-generation sequencing’s (NGS) potential discover genetic determinants. Fifty-five H. strains were successfully cultured screened for antibiotic susceptibility using agar dilution. Genotypic analysis was performed NGS data with a CLC genomic workbench. PlasmidSeeker detect plasmids. The correlation between resistant genotypes...
1. Purple speck, or “Shihan”, is caused by a fungus which new to science. The name, Cercosporina Kikuchii, suggested for this organism.2. also attacks leaves, stems and pods certain extent.3. When the grown on glucose media, it turns solutions purple. This mycelial extraction in water exhibits similar color, insoluble ether, petrol chloroform, benzine, terpentin oil carbon bisulphide. pigment becomes red with acid, pale greenish discoloration, when solution added alkali left few minutes.4....
Abstract Helicobacter pylori , a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to development genetically distinct H. subpopulations associated geographic origin host and differential disease risk. Here, we provide insights into population structure as part Genome Project ( Hp GP), multi-disciplinary initiative aimed at elucidating pathogenesis identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from...
We report 4 children with encephalitis associated human bocavirus (HBoV) 1 or 2. All were severely underweight, and 2 died; of them had a matching HBoV2 nucleotide sequence isolated from serum like particles in the cerebrospinal fluid that observed electron microscopy. No further pathogens detected these patients.
Helicobacter pylori (H. pylori) related chronic gastritis is a well-known major etiological factor for gastric cancer development. However, H. pylori-negative (HpN) not well described. We aimed to examine mucosal microbiota in HpN compared pylori-positive (HpP) and non-gastritis group (control). Here, we studied 11 subjects with HpN, 40 HpP 24 controls. performed endoscopy six biopsies. Comparison groups were defined based on strict histological criteria the disease diagnosis. used 16S rRNA...
Current management of gastric inflammation involves the eradication Helicobacter pylori. However, effectiveness commonly used antibiotics against H. pylori infection has decreased due to antibiotic resistance. Phenotypic-based diagnostics are laborious and finding cause resistance can be difficult. Therefore, early detection understanding underlying mechanism this necessary. This study evaluated mutations in genes related Antimicrobial Resistance (AMR) clinical isolates from Bangladeshi...
The number of copies rRNA (rrn) operons in a bacterial genome differs greatly among species. Here we examined the phenotypic effects variations genes Bacillus subtilis by analysis eight mutant strains constructed to carry from two nine rrn operon. We found that decrease ten one increased doubling time, and decreased sporulation frequency motility. maximum levels for transformation activity were similar strains, although competence development was significantly delayed strain with single...
We evaluated biofilm formation of clinical Helicobacter pylori isolates from Indonesia and its relation to antibiotic resistance. determined the minimum inhibition concentration (MIC) amoxicillin, clarithromycin, levofloxacin, metronidazole tetracycline by Etest measure planktonic susceptibility 101 H. strains. Biofilms were quantified crystal violet method. The eradication (MBEC) was obtained measuring survival bacteria in a after exposure antibiotics. majority strains formed (93.1%...
Antimicrobial susceptibility testing (AST) is increasingly needed to guide the Helicobacter pylori (H. pylori) treatment but remains laborious and unavailable in most African countries. To assess clinical relevance of bacterial whole genome sequencing (WGS)-based methods for predicting drug H. pylori, 102 strains isolated from Democratic Republic Congo were subjected phenotypic AST next-generation (NGS). WGS was used screen occurrence genotypes encoding antimicrobial resistance (AMR). We...
Helicobacter pylori (H. pylori) has been identified as a type I carcinogen and contributes to high rate of gastric cancer (GC), especially in Eastern Asia. Extracellular vesicles (EVs) have the potential be used detect various types diseases. However, protein markers EVs for prognosis H. infection GC are unknown. We aim identify proteins within derived from epithelial cell line (AGS) infected with by using LC-MS/MS. were isolated AGS cells high- low-virulence (strains TN2wt Tx30a)...
Background: As the global population ages, there is an increasing demand for effective strategies to maintain and improve health among older adults. Wearable technology presents a promising tool monitoring management, yet its effectiveness in comprehensive improvement adults remains uncertain. Objective: This study aimed evaluate of personalized lifestyle notifications, based on wearable device recorded data, improving outcomes, specifically cognitive physical function, adults, compared...
Abstract We identified human bocavirus (HBoV) DNA by PCR in cerebrospinal fluid from adults and children with encephalitis Sri Lanka. HBoV types 1, 2, 3 were among these cases. Phylogenetic analysis of HBoV1 strain sequences found no subclustering strains previously cases Bangladesh.
Recently a parvovirus called bufavirus (BuV) has been implicated as causative agent of diarrhoea. To further reveal the epidemiology and genetic characteristics BuV, this study was performed in Turkish children with BuV detected 1.4% (8/583) stool samples. All samples from healthy (n = 148) were negative for BuV. Diarrhoea BuV-positive patients severe occurred mainly during colder months year. Complete genome sequences generated four BuVs. Only BuV3 found, which genetically phylogenetically...
Mongolia has a high prevalence of Helicobacter pylori infection and the second highest incidence gastric cancer worldwide. Thus, investigating antibiotic resistance its underlying genetic mechanism is necessary. We isolated 361 H. strains throughout Mongolia. Agar dilution assays were used to determine minimum inhibitory concentrations five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, minocycline. The determinants identified with next-generation sequencing (NGS) CLC...
16S rRNA amplicon sequencing is an accurate method of detecting microbial infection without culture. It unclear if has additional benefits over routine diagnostic methods for Helicobacter pylori testing.We enrolled Mongolian volunteers with dyspepsia. Using methods, positive H. was defined as results on histology/immunohistochemistry, culture, rapid urease test, or serology; negative by from all these tests. We performed gastric biopsy specimens and calculated cutoffs operational taxonomic...
Patient-derived tumor organoids have considerable potential as an in vitro diagnostic tool for drug susceptibility testing. In the present study, we investigated whether bile collected purposes could be a source establishment of biliary cancer organoids. Among 68 cases cancer, successfully generated 60 bile-derived (BDOs) from individual patients. Consistent with previous reports that described surgical tissues, BDOs showed diverse morphologies such simple cysts, multiloculated thick...
Abstract Helicobacter pylori disturbs the stomach lining during long-term colonization of its human host, with sequelae including ulcers and gastric cancer 1,2 . Numerous H. virulence factors have been identified, showing extensive geographic variation 1 Here we identify a ‘Hardy’ ecospecies that shares ancestry ‘Ubiquitous’ from same region in most genome but has nearly fixed single-nucleotide polymorphism differences 100 genes, many which encode outer membrane proteins host interaction...
With first and second passage calli induced from seedlings of Digitalis purpurea, D. lanata, lutea, mertonensis, ambigua ferruginea Gigantea those leaf discs purpurea the contents digitoxin digoxin equivalents were assayed compared with involved in inocula. Although total varied between zero nine times as high original seedlings, almost undetectable. The approximately equal to discs, but less than In decreased slightly increased. However, calli, amounts both cardenolides decreased....