A5103144182- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Nosocomial Infections in ICU
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Microscopic Colitis
- Infective Endocarditis Diagnosis and Management
- Respiratory and Cough-Related Research
- Galectins and Cancer Biology
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Veterinary medicine and infectious diseases
- Bacterial biofilms and quorum sensing
- Oral microbiology and periodontitis research
Bar-Ilan University
2023-2024
Poriya Medical Center
2019-2023
Abstract Helicobacter pylori , a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to development genetically distinct H. subpopulations associated geographic origin host and differential disease risk. Here, we provide insights into population structure as part Genome Project ( Hp GP), multi-disciplinary initiative aimed at elucidating pathogenesis identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from...
Rapid and accurate pathogen identification in blood cultures is very important for septic patients has major consequences on morbidity mortality rates. In recent years, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)-based technology become useful highly specific sensitive bacteria yeasts from clinical samples including sterile body fluids. Additional in-house methods enabled direct following various preparation protocols. Blood culture (5 ml) was...
<title>Abstract</title> Background Even though they constitute a risk factor, antibiotics are still the current primary treatment of <italic>C. difficile</italic> infection. Due to difficile</italic>'s rapid development resistance and high recurrences rates, there is an unmet need for new antimicrobials. In study, we assessed in vitro susceptibility clinical isolates from Israel two recently developed antibiotics, ridinilazole ibezpolstat, currently used antibiotics. Methods We collected 313...
Antibiotics are currently the primary treatment of Clostridioides difficile (C. difficile) infection. Yet, due to rapid development resistance and high recurrences rates, there is an unmet need for new antimicrobials C. infections. This study assessed in vitro susceptibility clinical isolates from Israel two recently developed antibiotics, ridinilazole (RDZ) ibezapolstat (IBZ), standard-of-care antibiotics. (n = 313) recovered patients at both community hospital medical centers across...
To assess the biofilm-producing capacities of Staphylococcus aureus strains isolated from hospitalized patients in Israel.A total 16 S. (80 MRSA and 83 MSSA) screening (nasal swab) clinical samples (blood wounds) were characterized. Biofilm-producing determined using two different biofilm detection assays: Congo Red agar (CRA) microtiter plate (MtP). In addition, a real-time PCR analysis was performed to detect presence biofilm-associated genes (icaA icaD) mecA gene. The assays showed...
Background Clostridioides difficile ( C. ) is one of the primary pathogens responsible for infectious diarrhea. Antibiotic treatment failure, occurring in about 30% patients, and elevated rates antibiotic resistance pose a major challenge therapy. Reinfection often occurs by isolates that produce biofilm, protective barrier impermeable to antibiotics. We explored association between (in planktonic form) biofilm-production 123 clinical isolates. Results Overall, 66 (53.6%) out produced with...
Background: Clostridioides difficile infection (CDI) is a major nosocomial disease. The characteristics of different strains, the disease severity they cause, their susceptibility to antibiotics, and changes inflict on gut microbiome, have not been comprehensively studied in Israel. Methods: A score was calculated for 70 patients. Stool samples were tested toxins presence using special kit. Bacteria isolated, identified by matrix-assisted laser desorption ionization-time flight (MALDI-TOF)...
Abstract Background Clostridioides difficile ( C. ) is a major nosocomial pathogen that infects the human gut and can cause diarrheal disease. A dominant risk factor antibiotic treatment disrupts normal microbiota. The aim of study was to examine correlation between received prior infection (CDI) onset patient Methods Stool samples were collected from patients with CDI, presenting at Baruch Padeh Medical Center Poriya, Israel. Demographic clinical information, including previous treatments,...
Clostridioides difficile is one of the leading causes healthcare-associated diarrhea, with severity ranging from mild, self-limiting disease, to life-threatening toxic megacolon. C. infection (CDI) pathogenesis mediated by TcdA and TcdB toxins. This work aimed draw correlations between toxin levels, bacterial strains, disease in 63 CDI patients. typing was performed multi-locus sequence types (MLST). Toxin concentrations were measured using TOX A/B test. In addition, cell cytotoxicity assay...
Background The prevalence of community-acquired Clostridioides difficile infection (CA-CDI) has been rising, due to changes in antibiotics prescribing practices, emergence hypervirulent strains and improved diagnostics. This study explored CA-CDI epidemiology by examining strain diversity virulence factors isolates collected across several geographical regions Israel. Methods Stool samples 126 patients were subjected PCR an immunoassay identify toxin genes proteins, respectively. Toxin loci...
In recent years, associations between specific virulence markers of Helicobacter pylori (H. pylori) and gastrointestinal disorders have been suggested.To investigate the presence factors including vacuolating cytotoxin A genotypes (s1m1, s1m2, s2m1, s2m2), cytotoxin-associated gene (CagA), urease activity in H. strains isolated from Arab Jewish populations northern Israel to assess these patients' demographics clinical outcomes.Patients (n = 108) who underwent gastroscopy at Baruch Padeh...
Clostridioides difficile infection develops following ingestion of virulent stains by a susceptible host. Once germinated, toxins TcdA and TcdB, in some the strains binary toxin, are secreted, eliciting disease. Bile acids play significant role process spore germination outgrowth, with cholate its derivative enhancing colony formation, while chenodeoxycholate inhibit outgrowth. This work investigated bile acids' impact on germination, toxin levels biofilm formation various strain types...
Abstract Background : Clostridium difficile ( C. ) is a major nosocomial pathogen that infects the human gut and can cause diarrheal disease. A dominant risk factor antibiotic treatment disrupts normal microbiota. The aim of study was to examine correlation between received prior infection (CDI) onset patient Methods Stool samples were collected from patients with CDI, presenting at Baruch Padeh Medical Center Poriya, Israel. Demographic clinical information, including previous treatments,...