A5006988142- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Glycosylation and Glycoproteins Research
- Burkholderia infections and melioidosis
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Helicobacter pylori-related gastroenterology studies
- Enzyme Structure and Function
- Child Welfare and Adoption
- Bacterial biofilms and quorum sensing
- Listeria monocytogenes in Food Safety
- Mycobacterium research and diagnosis
- Galectins and Cancer Biology
- Brucella: diagnosis, epidemiology, treatment
- Attachment and Relationship Dynamics
- Genetics, Aging, and Longevity in Model Organisms
University of Alberta
2008-2023
Alberta Glycomics Centre
2012
ABSTRACT The Cpx two-component signal transduction pathway of Escherichia coli mediates adaptation to envelope protein misfolding. However, there is experimental evidence that at least 50 genes in 34 operons are part the regulon and many have functions undefined or unrelated maintenance. No comprehensive analysis has been presented date. In order identify strongly Cpx-regulated might play an important role(s) folding and/or further define role response gain insight into what makes a gene...
The Cpx envelope stress response mediates adaptation to stresses that cause protein misfolding. Adaptation is partly conferred through increased expression of folding and degradation factors. also plays a conserved role in the regulation virulence determinant impacts antibiotic resistance. We sought identify adaptive mechanisms may be involved these important functions by characterizing changes transcriptome two different Escherichia coli strains when induced. show that, while there...
The World Health Organization has indicated that we are entering into a post-antibiotic era in which infections were routinely and successfully treated with antibiotics can now be lethal due to the global dissemination of multidrug resistant strains. Conjugate vaccines an effective way create long-lasting immune response against bacteria. However, these present many drawbacks such as slow development, high price, batch-to-batch inconsistencies. Alternate approaches for vaccine development...
Abstract Helicobacter pylori , a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to development genetically distinct H. subpopulations associated geographic origin host and differential disease risk. Here, we provide insights into population structure as part Genome Project ( Hp GP), multi-disciplinary initiative aimed at elucidating pathogenesis identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from...
Vaccines developing immune responses towards surface carbohydrates conjugated to proteins are effective in preventing infection and death by bacterial pathogens. Traditional production of these vaccines utilizes complex synthetic chemistry acquire conjugate the glycan a protein. However, glycoproteins produced protein glycosylation systems significantly easier produce, could possible be used as vaccine candidates. In this work we functionally expressed B. pseudomallei O polysaccharide (OPS...
Bacterial protein glycosylation systems from varying species have been functionally reconstituted in Escherichia coli. Both N- and O-linked pathways, which the glycans are first assembled onto lipid carriers subsequently transferred to acceptor proteins by an oligosaccharyltransferase (OTase), documented bacteria. The identification characterization of novel OTases with different properties may provide new tools for engineering glycoproteins biotechnological interest. In case involved...
Abstract Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a system in given microbe also hampers genetic analysis. Although plasmids can be successfully conjugated into enteropathogenic Escherichia coli strain E2348/69 and optimized protocols for competent cell preparation have been developed, we found large, low copy (~15) bioluminescent reporter plasmid, pJW15, modified use EPEC, was exceedingly...