A5049578807- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Veterinary medicine and infectious diseases
- Mycobacterium research and diagnosis
- Gastrointestinal disorders and treatments
- Colorectal Cancer Treatments and Studies
- Genomics, phytochemicals, and oxidative stress
- RNA and protein synthesis mechanisms
- Gastric Cancer Management and Outcomes
- Celiac Disease Research and Management
- Plant Stress Responses and Tolerance
- Glutathione Transferases and Polymorphisms
- Microbial Metabolites in Food Biotechnology
Van Lang University
2025
Oita University
2019-2023
Cho Ray Hospital
2021-2023
University of Tsukuba
2011
We evaluated the primary resistance of Helicobacter pylori (H. pylori) to routinely used antibiotics in Cambodia, an unexplored topic country, and assessed next-generation sequencing’s (NGS) potential discover genetic determinants. Fifty-five H. strains were successfully cultured screened for antibiotic susceptibility using agar dilution. Genotypic analysis was performed NGS data with a CLC genomic workbench. PlasmidSeeker detect plasmids. The correlation between resistant genotypes...
Helicobacter pylori infects at least half the population worldwide, and its highly diverse genomic content correlates with geographic distribution, because of prolonged relationship humans. The extremely low infection prevalence alongside inflammation severity observed in some countries might be caused by strains virulence potential. Therefore, this study aimed to investigate whole genome analysis datasets Sri Lankan H. strains. isolated from biopsy specimens underwent sequencing their...
Helicobacter pylori infects at least half the population worldwide, and its highly diverse genomic content correlates with geographic distribution because of prolonged relationship humans. The extremely low infection prevalence alongside inflammation severity observed in some countries might be caused by strains virulence potential. Therefore, this study aimed to investigate whole-genome analysis datasets Sri Lankan H. strains. were isolated from biopsy specimens underwent sequencing their...
Genome-wide association studies (GWASs) can reveal genetic variations associated with a phenotype in the absence of any hypothesis candidate genes. The problem false-positive sites linked responsible site might be bypassed bacteria high homologous recombination rate, such as Helicobacter pylori, which causes gastric cancer. We conducted small-sample GWAS (125 cancer cases and 115 controls) followed by prediction control (duodenal ulcer) H. pylori strains. identified 11 single nucleotide...
Abstract Although the type 4 secretion system of integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori , its clinical association with cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. samples (46 duodenal ulcer (DU), 51 non-cardia gastric cancer (NCGC), 39 chronic gastritis (CG)) were fully sequenced using next-generation sequencing assembled into contigs. tfs3, tfs4, cagPAI genes compared public database....
Abstract Helicobacter pylori is involved in the etiology and severity of several gastroduodenal diseases; however, plasticity H. genome makes complete assembly difficult. We report here full genomes strains CHC155 VN1291 isolated from a non-cardia gastric cancer patient duodenal ulcer patient, respectively, their virulence demonstrated by vitro infection. Whole-genome sequences were obtained combining long- short-reads with hybrid-assembly approach. Both possessed four kinds genomic island:...
The use of serum anti-Helicobacter pylori IgG and pepsinogen (PG) detection as a diagnostic method was evaluated in Sri Lanka. Gastric biopsies were performed (353 patients), the prevalence H. infection 1.7% (culture) 2.0% (histology). serology testing showed an area under curve (AUC) 0.922 (cut-off, 2.95 U/mL; specificity, 91.56%; sensitivity, 88.89%). Histological evaluation mild atrophy (34.3%), moderate (1.7%), metaplasia chronic gastritis (6.2%), normal tissue (56%). PGI/PGII ratio...
Beside diagnostic uncertainties due to the lack of a perfect gold standard test for Helicobacter pylori infection, diagnosis and prevalence estimation this infection encounter particular challenges in Africa including limited tools specific genetic background. We developed evaluated accuracy an enzyme-linked immunosorbent assay (ELISA) system tailored H . genetics (HpAfr-ELISA). Strains belonging main populations infecting Africans were exploited as sources whole-cell antigens establish...
Background and Aim To determine the application range of diagnostic kits utilizing anti-Helicobacter pylori antibody, we tested a newly developed latex aggregation turbidity assay (latex) conventional enzyme-linked immunosorbent (E-plate), both containing Japanese H. protein lysates as antigens, using sera from seven Asian countries. Methods Serum samples (1797) were obtained, standard infection status atrophy determined by culture histology (immunohistochemistry) gastric biopsy same...
Abstract Genome-wide association studies (GWASs) can reveal genetic variations associated with a phenotype in the absence of any hypothesis candidate genes. The problem false-positive sites linked responsible site might be bypassed bacteria high homologous recombination rate, such as Helicobacter pylori , which causes gastric cancer (GC). We conducted GWAS followed by regression-based prediction GC and duodenal ulcer H. strains. identified 14 single nucleotide polymorphisms (11 amino acid...