A5020786339- Metabolomics and Mass Spectrometry Studies
- Helicobacter pylori-related gastroenterology studies
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Toxic Organic Pollutants Impact
- Pancreatic and Hepatic Oncology Research
- Analytical Chemistry and Chromatography
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Effects and risks of endocrine disrupting chemicals
- Diet and metabolism studies
- Advanced Chemical Sensor Technologies
- Tea Polyphenols and Effects
- Carcinogens and Genotoxicity Assessment
- Galectins and Cancer Biology
- Adipose Tissue and Metabolism
- Veterinary medicine and infectious diseases
- Pancreatitis Pathology and Treatment
- Fatty Acid Research and Health
- Metabolism, Diabetes, and Cancer
- Mass Spectrometry Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Drug Transport and Resistance Mechanisms
- Nutrition, Genetics, and Disease
- Peroxisome Proliferator-Activated Receptors
Kobe University
2013-2023
Hyogo Medical University
2020-2023
Kobe College
2023
Weatherford College
2008-2020
Japan Agency for Medical Research and Development
2020
In-Q-Tel
2017
Kobe University Hospital
2013
Nippon Medical School
2013
Chiang Mai University
2013
Hyogo Prefectural Cancer Center
2013
Background To improve the quality of life colorectal cancer patients, it is important to establish new screening methods for early diagnosis cancer. Methodology/Principal Findings We performed serum metabolome analysis using gas-chromatography/mass-spectrometry (GC/MS). First, accuracy our GC/MS-based metabolomic analytical method was evaluated by calculating RSD% values levels various metabolites. Second, intra-day (morning, daytime, and night) inter-day (among 3 days) variances metabolite...
Tumor recurrence is a major clinical issue that represents the principal cause of cancer-related deaths, with few targetable common pathways. Mechanisms by which residual tumors persist and progress under continuous shift between hypoxia-reoxygenation after neoadjuvent-therapy are unknown. In this study, we investigated role lipid metabolism tumor redox balance in recurrence. Lipidomics, proteomics mass spectrometry imaging approaches where applied to mouse models Genetic pharmacological...
Aim: Paracrine interaction between macrophages and adipocytes in obese visceral fat tissues is thought to be a trigger of chronic inflammation. The immunomodulatory effect the short chain fatty acid, butyric has been demonstrated. We hypothesize that sodium butyrate (butyrate) attenuates inflammatory responses lipolysis generated by adipocytes.Methods: Using contact or transwell co-culture methods with differentiated 3T3-L1 RAW264.7 macrophages, we investigated effects on production tumor...
ABSTRACT Conventional tumor markers are unsuitable for detecting carcinoma at an early stage and lack clinical efficacy utility. In this study, we attempted to investigate the differences in serum metabolite profiles of gastrointestinal cancers healthy volunteers using a metabolomic approach searched sensitive specific biomarker candidates. Human samples were obtained esophageal ( n = 15), gastric 11), colorectal 12) cancer patients 12). A model evaluating candidates was constructed multiple...
Resolvin E1 (RvE1), an endogenous lipid mediator derived from eicosapentaenoic acid, has been identified in local inflammation during the healing stage. RvE1 reduces several types of animal models including peritonitis and retinopathy blocks human neutrophil transendothelial cell migration. The receptor ChemR23 is expressed on myeloid cells such as macrophages dendritic cells. aim this study was to determine whether regulates colonic when innate immune response plays a key role pathogenesis...
To improve the prognosis of patients with pancreatic cancer, more accurate serum diagnostic methods are required. We used metabolomics as a method for cancer.Sera from healthy volunteers, and chronic pancreatitis were collected at multiple institutions. The cancer volunteers randomly allocated to training or validation set. All cases included in In each study, subjects' metabolites analyzed by gas chromatography mass spectrometry (GC/MS) data processing system using an in-house library....
Abstract CagA, encoded by cytotoxin-associated gene A ( cagA ), is a major virulence factor of Helicobacter pylori , gastric pathogen involved in the development upper gastrointestinal diseases. Infection with -positive H. may also be associated diseases outside stomach, although mechanisms through which infection promotes extragastric remain unknown. Here, we report that CagA present serum-derived extracellular vesicles, known as exosomes, patients infected (n = 4). We found epithelial...
The aim of this study was to establish a rapid preparation plasma membrane from adipocytes and muscle cells detect translocated glucose transporter (GLUT) 4. A fraction prepared by sequential centrifugation with buffer containing detergents, its purity estimated detecting insulin receptor β-subunit (IRβ). After stimulus, GLUT4 translocation observed in 3T3-L1 L6 myotubes. It found that IRβ levels on the decreased adipose intake 29% lard diet for 14 weeks. Hence, method should be useful fraction.
Metabolomics provides data about all the metabolic processes of a cell or organism. So far, changes that occur in levels metabolites during development colitis have not been fully elucidated. Here we examined metabolite serum and colon tissue mice using gas chromatography mass spectrometry (GC/MS) with aim achieving detailed understanding pathogenesis inflammatory bowel disease (IBD). To induce colitis, C57BL/6J were administered 3.0% dextran sulfate sodium (DSS) their drinking water for 5...
Metabolomics has recently undergone rapid development; however, metabolomic analysis in cerebrospinal fluid (CSF) is not a common practice. We analyzed the metabolite profiles of preoperative CSF samples from 32 patients with histologically confirmed glioma using gas chromatography/mass spectrometry (GC/MS). assessed how alterations levels were related to World Health Organization (WHO) tumor grades, location, gadolinium enhancement on magnetic resonance imaging (MRI), and isocitrate...
In developed countries, the number of patients with colorectal cancer has been increasing, and is one most common causes death. To improve quality life patients, it necessary to establish novel screening methods that would allow early detection cancer. We performed metabolome analysis a plasma sample set from 282 stage 0/I/II 291 healthy volunteers using gas chromatography/triple-quadrupole mass spectrometry in an attempt identify metabolite biomarkers The included 0 (N=79), I (N=80), II...
Triple-negative breast cancer (TN) is more aggressive than other subtypes of and has a lower survival rate. Furthermore, detailed biological information about the disease lacking. This study investigated characteristics metabolic pathways in TN. We performed metabolome analysis 74 tissues corresponding normal using LC/MS. we classified into ER-positive, PgR-positive, HER2-negative (EP+H-) TN, then differences their were investigated. The RT-PCR immunostaining carried out to examine...