A5087296924- Esophageal Cancer Research and Treatment
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- NF-κB Signaling Pathways
- RNA modifications and cancer
- Helicobacter pylori-related gastroenterology studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Plant Physiology and Cultivation Studies
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- melanin and skin pigmentation
- Signaling Pathways in Disease
- Inflammatory Bowel Disease
- Galectins and Cancer Biology
- Glutathione Transferases and Polymorphisms
- Digestive system and related health
- Gut microbiota and health
- Sperm and Testicular Function
- Flowering Plant Growth and Cultivation
- Mercury impact and mitigation studies
- IL-33, ST2, and ILC Pathways
- Glycosylation and Glycoproteins Research
- Plant Reproductive Biology
- Genetic factors in colorectal cancer
Kindai University
2006-2024
National Center for Global Health and Medicine
2013-2023
Center for Global Health
2017-2018
Yokohama City University
1999-2009
Saitama International Medical Center
2008
Saitama Institute of Technology
1993-2006
Horticultural Research Institute
1939
Peptidylarginine deiminase (PAD) deiminates arginine residues in proteins to citrulline Ca2+ dependently. There are four types of PADs, I, II, III, and V, humans. We studied the subcellular distribution PAD V HL-60 granulocytes peripheral blood granulocytes. Expression green fluorescent protein-tagged PADs HeLa cells revealed that is localized nucleus, whereas III cytoplasm. deletion mutants indicated sequence 45–74 have a nuclear localization signal (NLS). A feature this NLS three-lysine...
Three types of peptidylarginine deiminase (PAD), which converts a protein arginine residue to citrulline residue, are widely distributed in animal tissues. Little is known about PAD hemopoietic cells. We found that activity human myeloid leukemia HL-60 cells was induced with the granulocyte-inducing agents retinoic acid and dimethyl sulfoxide monocyte-inducing agent 1α,25-dihydroxyvitamin D3. cloned characterized cDNA from acid-induced The 2,238 base pairs long encoded 663-amino polypeptide....
Interplay of various covalent modifications histone tails has an essential role in regulation chromatin function. Peptidylarginine deiminase (PADI) 4 deiminates protein arginine to citrulline a Ca(2+)-dependent manner and is present the nucleus granulocyte-differentiated HL-60 cells. When these cells are treated with calcium ionophore A23187, core deimination occurs. To determine sites histones, species were purified by reverse-phase high-performance liquid chromatography (RP-HPLC) from...
Chemokine (C-C motif) receptor 8 (CCR8), the chemokine for ligand 1 (CCL1), is expressed in T-helper type-2 lymphocytes and peritoneal macrophages (PMφ) involved various pathological conditions, including adhesions. However, role of CCR8 inflammatory responses not fully elucidated. To investigate function macrophages, we compared cytokine secretion from mouse PMφ or bone marrow-derived (BMMφ) stimulated with Toll-like (TLR) ligands deficient (CCR8-/-) wild-type (WT) mice. We found that...
Peptidylarginine deiminase IV (PAD IV) catalyzes the citrullination of Arg residues proteins, such as histones. Suzuki et al. recently reported that haplotypes PAD gene are associated with susceptibility to rheumatoid arthritis. To investigate mechanism substrate specificity and inhibitors IV, a series derivatives were synthesized their reactivity examined. The results suggest both imino carboxyl groups important in molecular recognition methylation guanidino group prevents citrullination....
// Takeshi Otsubo 1 , Kazuhiko Yamada 2 Teruki Hagiwara Kenshiro Oshima 3 Kei Iida 4 Koro Nishikata 5 Tetsuro Toyoda Toru Igari 6 Kyoko Nohara Satoshi Yamashita Masahira Hattori 3, 7 Taeko Dohi and Yuki I. Kawamura Department of Gastroenterology, The Research Center for Hepatitis Immunology, Institute, National Global Health Medicine, Chiba, Japan Surgery, Tokyo, Computational Biology Medical Sciences, Graduate School Frontier Science, University Support Center, Kyoto University, Kyoto,...
Abstract Periplakin (PPL), a member of the plakin family proteins that localizes to desmosomes and intermediate filaments, is downregulated in human esophageal squamous cell carcinoma (ESCC). Little known, however, about molecular mechanism underlying regulation PPL expression contribution loss malignant property cancer unclear. We demonstrated mRNA was significantly reduced ESCC tissues compared with normal tissues. Therefore, we hypothesized CpG hypermethylation cause downregulation PPL....
Peptidylarginine deiminases (PADs) consist of five enzymes which are widely distributed in human and rodent tissues. The two types found peripheral blood cells; PAD4 mainly granulocytes monocytes PAD2 lymphocytes macrophages. Little is known about the regulation PAD expression Here, we report that expressed monocytic leukaemia THP-1 cells during differentiation into macrophages by 12-O-tetradecanoylphorbol-13-acetate. During this differentiation, levels mRNA protein increased concomitantly,...
We previously conducted transcriptome analysis of a paired specimen normal and esophageal squamous cell carcinoma (ESCC) tissues found that mRNA expression cystatin A (CSTA), member the superfamily, was perturbed in tumors compared with background mucosa. However, little is known about significance CSTA ESCC.The evaluated by qRT-PCR using 28 frozen samples tumor nontumor mucosae. The protein immunostaining formalin-fixed, paraffin-embedded sections ESCC from 59 patients who underwent...
Fasting-refeeding in mice induces transient hyperproliferation of colonic epithelial cells, which is dependent on the lactate produced as a metabolite commensal bacteria. We attempted to manipulate cell turnover with intermittent fasting prompt recovery from acute colitis. Acute colitis was induced C57BL/6 by administration dextran sulfate sodium drinking water for 5 days. From day 6, were fasted 36 h and refed normal bait, glucose powder, or lactylated high-amylose starch. On 9, colon...
Abstract The biological catalysts adsorbed on the carbon surface gradually lost their activities. In order to prolong stabilization of enzyme activity, polymaleimidostyrene (PMS) was used preserve conformation. Sulfhydryl and amino groups or tissue can be covalently bound PMS. By using material coated by PMS, stability L‐ascorbic acid sensor response purified significantly improved, its initial not decreased at all for 150 days, although enzymes without PMS activity 22.5% after 10 days.
Glutathione S-transferase omega 2 (GSTO2) lacks any appreciable GST activity, but it exhibits thioltransferase activity. The significance of GSTO2 in lung function has been reported; however, the precise expression and molecular lungs remain unclear. In present study, we found that is expressed airway basal cells, non-ciliated, columnar Clara type II alveolar which have self-renewal capacity lungs. Contrastingly, no was observed 94 squamous cell carcinoma (LSCC) samples. When human LSCC...
To clarify the roles of TWEAK and its receptor Fn14 in 5-fluorouracil (5-FU)-induced diarrhea.Diarrhea was induced wild-type (WT), knockout (KO), IL-13 (IL-13R)α1 KO BALB/c mice using a single injection 5-FU. Histological analysis, cytokine flow cytometry performed on ileal tissues cells. Murine colon carcinoma-bearing were co-treated with an anti-TWEAK antibody Embryonic fibroblast response to cytokines also analyzed.5-FU high expression epithelial The severity 5-FU-induced diarrhea lower...