A5035038653- Retinoids in leukemia and cellular processes
- Pancreatic function and diabetes
- Cellular transport and secretion
- Antioxidant Activity and Oxidative Stress
- Adipose Tissue and Metabolism
- Bacterial Genetics and Biotechnology
- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Calcium signaling and nucleotide metabolism
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Peroxisome Proliferator-Activated Receptors
- Metabolism, Diabetes, and Cancer
- CRISPR and Genetic Engineering
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Diabetes and associated disorders
- Receptor Mechanisms and Signaling
- Aortic aneurysm repair treatments
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Cardiac, Anesthesia and Surgical Outcomes
- Biotin and Related Studies
- Carbohydrate Chemistry and Synthesis
The University of Tokyo
2011-2024
Kitasato University
2018-2024
Showagakuin Junior College
2006-2024
Hoshi University
2015-2024
Ebara (Japan)
2005-2024
Kyorin University
2009-2023
National Cancer Center
2008-2022
Bayer (Japan)
2021
Koriyama Institute of Health Sciences
2021
Nagoya University
2006-2020
Significance Substantial knowledge exists about how antibiotics interfere with core bacterial processes by binding to specific targets. Recently it has become appreciated that blocking these functions alters cellular redox state, and perturbations may contribute the lethality of antibiotics. In this work we explore whether antibiotic treatment bacteria affects oxidative stress role such in antibiotic-mediated killing. We find dynamically alter respiration induce lethal levels intracellular...
Glucose metabolism in glycolysis and mitochondria is pivotal to glucose-induced insulin secretion from pancreatic β cells. One or more factors derived other than pyruvate appear be required for the generation of mitochondrial signals that lead secretion. The electrons glycolysis-derived reduced form nicotinamide adenine dinucleotide (NADH) are transferred through NADH shuttle system. By abolishing function, increases autofluorescence, membrane potential, adenosine triphosphate content were...
Insulin secretion from intact mouse pancreatic islets was investigated with two-photon excitation imaging. granule exocytosis occurred mainly toward the interstitial space, away blood vessels. The fusion pore unusually stable a lifetime of 1.8 seconds. Opening 1.4-nanometer-diameter preceded by unrestricted lateral diffusion lipids along inner wall pore, supporting idea that this structure is composed membrane lipids. When dilated to 12 nanometers, granules rapidly flattened and discharged...
Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-catabolising enzyme inducing immune tolerance. The present study aimed to investigate IDO expression and its prognostic significance in endometrial cancer. cancer tissues (n=80) was immunohistochemically scored as four groups (IDO−, 1+, 2+, 3+). high (IDO2+ or 3+) tumour cells found 37 (46.3%) of the 80 cases, positively correlated with surgical stage, myometrial invasion, lymph-vascular space involvement, lymph node metastasis, but not...
Mice carrying a null mutation in the glucokinase (GK) gene pancreatic β-cells, but not liver, were generated by disrupting β-cell-specific exon. Heterozygous mutant mice showed early-onset mild diabetes due to impaired insulin-secretory response glucose. Homozygotes severe shortly after birth and died within week. GK-deficient islets isolated from homozygotes defective insulin secretion glucose, while they responded other secretagogues: almost normally arginine some extent sulfonylureas....
Abstract Background The genome of Helicobacter pylori , an oncogenic bacterium in the human stomach, rapidly evolves and shows wide geographical divergence. high incidence stomach cancer East Asia might be related to bacterial genotype. We used newly developed comparative methods follow evolution Asian H. genomes using 20 complete sequences from Japanese, Korean, Amerind, European, West African strains. Results A phylogenetic tree concatenated well-defined core genes supported divergence...
The urinary concentrations of 8-isoprostane and 8-hydroxy-2'-deoxyguanosine (8-OHdG), which are biomarkers oxidative stress, were measured in 677 Japanese people without any diseases, their correlations with lifestyle facotrs, lifestyle-related blood biochemical parameters, dietary intake antioxidative vitamins investigated. mean concentration 8-OHdG was 0.58 ng/mg creatinine 8.43 creatinine, respectively. Mean significantly different terms age, gender, smoking alcohol consumption but not...
Follicular helper T (Tfh) cells access the B cell follicle to promote antibody responses and are particularly important for germinal center (GC) reactions. However, molecular mechanisms of how Tfh physically associated with GCs incompletely understood. We report that sphingosine-1-phosphate receptor 2 (S1PR2) gene is highly expressed in a subpopulation localizes GCs. S1PR2-deficient exhibited reduced accumulation due their impaired retention. deficient both S1PR2 CXCR5 were ineffective...
Significance This work, for the first time to our knowledge, distinctly visualizes two different populations of dendritic cells (DCs) essential cytotoxic T-cell generation in skin-draining lymph nodes (SDLNs): migratory CD103 hi DCs that immigrate from other organs including skin and CD8α are resident SDLNs. By imaging spatiotemporal dynamics subsets SDLNs, we find these play roles antigen presentation, with being dramatically more potent interacting CD8 + T cells. work offers critical...
Increased levels of lactate, an end-product glycolysis, have been proposed as a potential surrogate marker for metabolic changes during neuronal excitation. These in lactate can result decreased brain pH, which has implicated patients with various neuropsychiatric disorders. We previously demonstrated that such alterations are commonly observed five mouse models schizophrenia, bipolar disorder, and autism, suggesting shared endophenotype among these disorders rather than mere artifacts due...
Abstract Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (TTSS) to inject effector proteins into the plasma membrane and cytosol of infected cells. To translocate proteins, EPEC, like Salmonella Shigella, is believed assemble macromolecular complex (type secreton) that spans both bacterial membranes has short needle-like projection. However, there special interest in studying EPEC TTSS owing fact one secreted EspA, assembled unique filamentous structure also...
The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles. Although natural mutations of the gene in human Griscelli syndrome ashen mice cause partial albinism immunodeficiency reflecting dysfunction organelles, phenotypes resulting from defective exocytosis have not been reported. To explore roles granules, we analyzed insulin secretion profiles mice. Ashen showed glucose intolerance after a load without signs resistance...
The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles. Although natural mutations of the gene in human Griscelli syndrome ashen mice cause partial albinism immunodeficiency reflecting dysfunction organelles, phenotypes resulting from defective exocytosis have not been reported. To explore roles granules, we analyzed insulin secretion profiles mice. Ashen showed glucose intolerance after a load without signs resistance...
The role of cytosolic ATP in exocytosis was investigated by using amperometric measurement insulin pancreatic beta cells, which were stimulated with photolysis caged Ca2+ compounds. Insulin occurred two rates. We found that hastened and augmented the via selective enhancement faster rate. A nonhydrolysable analog ATP, adenosine 5'-O-(3-thiotriphosphate), blocks ATPase, even more effective than indicating phosphorylation event downstream ATP-dependent vesicle transportation priming. action...
N-Phenyl-N'-{4-(4-quinolyloxy)phenyl}ureas were found to be a novel class of potent inhibitors for the vascular endothelial growth factor receptor 2 (VEGFR-2) tyrosine kinase through synthetic modifications lead compound and structure−activity relationship studies. A representative 6ab, termed Ki8751, inhibited VEGFR-2 phosphorylation at an IC50 value 0.90 nM, also PDGFR family members such as PDGFRα c-Kit 67 nM 40 respectively. However, 6ab did not have any inhibitory activity against other...