A5056577823- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Genetics, Bioinformatics, and Biomedical Research
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- Microbial metabolism and enzyme function
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Esophageal and GI Pathology
- Glycosylation and Glycoproteins Research
- Folate and B Vitamins Research
- Biofuel production and bioconversion
- Cancer Research and Treatments
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
Madurai Kamaraj University
2006-2010
Weizmann Institute of Science
2006
YagE is a 33 kDa prophage protein encoded by CP4-6 element in Escherichia coli K12 genome. Here, we report the structures of complexes with pyruvate (PDB Id 3N2X) and KDGal (2-keto-3-deoxy galactonate) 3NEV) at 2.2A resolution. Pyruvate depletion assay presence glyceraldehyde shows that catalyses aldol condensation glyceraldehyde. Our results indicate biochemical function 2-keto-3-deoxy gluconate (KDG) aldolase. Interestingly, E. genome lacks an intrinsic KDG Moreover, over-expression...
The incorporation of genetic material from other organisms into bacterial genomes potentiates novel forms drug resistance or pathogenicity. One method is through infection by a bacteriophage, which enters the lysogenic life cycle. Excision phage genome as result induction may include some host's genome, and subsequent lysogeny in bacteria would incorporate this nonphage DNA these hosts. Loss ability to excise itself host leads permanent addition genes that have accumulated during previous...