A5002502576- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Amino Acid Enzymes and Metabolism
- Crystallography and molecular interactions
- Cancer, Hypoxia, and Metabolism
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Plant Physiology and Cultivation Studies
- Cancer Research and Treatments
- Advanced Proteomics Techniques and Applications
- Amyotrophic Lateral Sclerosis Research
- Flowering Plant Growth and Cultivation
- Enzyme Production and Characterization
- Biochemical and biochemical processes
- Plant Gene Expression Analysis
- Folate and B Vitamins Research
- Microbial metabolism and enzyme function
- Particle accelerators and beam dynamics
Friedrich Miescher Institute
2019-2021
Max Planck Society
2013-2015
Max Planck Institute of Biochemistry
2013
Indian Institute of Technology Kanpur
2012
Centre for Cellular and Molecular Biology
2011
Madurai Kamaraj University
2010
University of Illinois Chicago
2008
The IGF2 mRNA-binding proteins (ZBP1/IMP1, IMP2, IMP3) are highly conserved post-transcriptional regulators of RNA stability, localization and translation. They play important roles in cell migration, neural development, metabolism cancer survival. knockout phenotypes individual IMP suggest that each family member regulates a unique pool RNAs, yet evidence an underlying mechanism for this is lacking. Here, we combine systematic evolution ligands by exponential enrichment (SELEX) NMR...
Ribosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of human 80S ribosome in post-decoding pre-translocation state (classical-PRE) at 3.3-Å resolution along with rotated (hybrid-PRE) and post-translocation states (POST). The classical-PRE reveals a previously unobserved interaction between C-terminal region conserved ribosomal protein uS19 A- P-site tRNAs mRNA decoding site. In...
The Ccr4-Not complex regulates eukaryotic gene expression at multiple levels, including mRNA turnover, translational repression, and transcription. We have studied the ubiquitylation module of yeast addressed how E3 ligase binds cognate E2 it is tethered to complex. 2.8-Å resolution crystal structure N-terminal RING domain Not4 in with Ubc4 shows detailed interactions this E3-E2 3.6-Å C-terminal Not1 reveals a largely extended region C-terminus wraps around HEAT-repeat Not1. This only partly...
Ribosomes are the macromolecular machines at heart of protein synthesis; however, their function can be modulated by a variety additional factors that directly interact with them. Here, we report cryo-EM structure human Ebp1 (p48 isoform) bound to 80S ribosome 3.3 Å resolution. binds in vicinity peptide exit tunnel on ribosome, and this binding is enhanced upon puromycin-mediated translational inhibition. The association centers around its interaction ribosomal proteins eL19 uL23 28S rRNA....
Subtilisin (EC 3.4.21.62) is a type of serine protease that high commercial importance. It mainly produced by Bacillus species as an extra cellular enzyme. being stable over wide range pH and temperature exploited detergent enzyme also favoured its non-specificity. The yield this needs to be optimized for cost effective production subtilisin commercialization. Here, various solid substrates were screened the using subtilis MTCC 441 maximum was found with green gram husk. Response surface...
YagE is a 33 kDa prophage protein encoded by CP4-6 element in Escherichia coli K12 genome. Here, we report the structures of complexes with pyruvate (PDB Id 3N2X) and KDGal (2-keto-3-deoxy galactonate) 3NEV) at 2.2A resolution. Pyruvate depletion assay presence glyceraldehyde shows that catalyses aldol condensation glyceraldehyde. Our results indicate biochemical function 2-keto-3-deoxy gluconate (KDG) aldolase. Interestingly, E. genome lacks an intrinsic KDG Moreover, over-expression...
A major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) spectrum disorder is the hexanucleotide G 4 C 2 repeat expansion in first intron C9orf72 gene. Many underlying mechanisms lead to manifestation disease that include toxic gain-of-function by RNAs, dipeptide proteins, a reduction gene product. The protein interacts with SMCR8 WDR41 form trimeric complex regulates multiple cellular pathways including autophagy. Here, we report structure...
Abstract The Igf2 mRNA binding proteins (ZBP1/IMP1, IMP2, IMP3) are highly conserved post-transcriptional regulators of RNA stability, localization and translation. They play important roles in cell migration, neural development, metabolism cancer survival. knockout phenotypes individual IMP suggest that each family member regulates a unique pool RNAs, yet evidence an underlying mechanism for this is lacking. Here, we combine SELEX NMR spectroscopy to demonstrate the major domains two most...
EngA is an essential protein involved in ribosome biogenesis. It unique GTPase, possessing two consecutive G-domains. Using sequence and phylogenetic analysis, we found intriguing variants among homologues - one with a shorter linker joining the G-domains another longer linker, which additionally possesses extended C-terminus. Interestingly, while former variant mainly restricted to firmicutes, latter nonfirmicutes. Chimeric proteins interchanged linkers extensions were generated gauge...