The prospect of building a future energy system on hydrogen has stimulated much research effort in developing storage technologies. One the potential materials newly developed is sodium amidoborane (NaNH2BH3) which evolves ∼7.5 wt% at temperatures as low 91 °C. In this paper, two methods synthesizing pure NaNH2BH3 were reported. method by reacting NaH and ammonia borane THF temperatures, other NaNH2 ambient temperature. Non-isothermal testing thermolysis solid showed that evolution was...

Abstract Polymerase chain reaction (PCR) has been a defining tool in modern biology. Towards realizing mirror-image PCR, we have designed and chemically synthesized mutant version of the 352-residue thermostable Sulfolobus solfataricus P2 DNA polymerase IV with l -amino acids tested its PCR activity biochemically. To best our knowledge, this enzyme is largest protein reported to date. We show that optimization conditions, fully synthetic capable amplifying template sequences up 1.5 kb. The...

The two-step reaction between LiH and NH3BH3 in THF leads to the production of more than 14 wt% hydrogen at 40 °C.

Lithium amidoborane (LiNH2BH3) and ammonia borane (NH3BH3) reduce aromatic aldehydes in tetrahydrofuran (THF) through two different pathways. LiNH2BH3 only transfers hydridic hydrogen on boron to a hydroboration process achieve lithium aminoborate; borane, the other hand, both protic hydrogens N B, respectively, directly corresponding alcohols. Mechanistic investigations confirm that H(N) H(B) of participate reduction, which dissociation B–H N–H bonds is likely be involved rate-determining step.

The authentication and labeling of meat products, concerning origins species, are key to fair trade protect consumer interests in the market. We developed an improved triplex real-time PCR approach simultaneously identify chicken, duck, goose DNA meat, including endogenous control avoid false negatives. Our method specifically detected from goose, showed no cross-reaction with extracted other types. detection limits were 0.001-0.00025 ng, 0.0025-0.0001 0.001-0.00001 respectively, we able two...

Abstract Mare milk originated from female horses, known as mares, to feed their foals during lactation. The health‐promoting characteristics of traditionally fermented mare (Chigee) are well for the function clinic treatment in traditional Mongolian medicine. This study was conducted investigate production technology Chigee and evaluate nutritional microbiological based on 188 samples. analysis indicated that lactose significantly decreased 6.95 ± 0.45% 2.82 1.65% acidity alcoholic content...

Abstract Metal amidoboranes (MABs), such as lithium amidoborane (LiAB), show superior ability in reducing ketones and imines directly into their corresponding secondary alcohols amines, respectively, at room temperature with high conversion yields. A mechanistic study indicates that the reduction proceeds through a double‐hydrogen‐transfer process. Both protic H(N) hydridic H(B) protons participate reaction. Theoretical investigations first (and rate‐determining) step of reaction is...

Lithium amidoborane (LiNH(2)BH(3), LiAB for short), is capable of chemoselectively reducing α,β-unsaturated ketones to the corresponding allylic alcohols at ambient temperature. A mechanistic study shows that reduction via a double hydrogen transfer process. The protic H(N) and hydridic H(B) in add O C sites carbonyl group, respectively.

Abstract Due to their outstanding nutritional and functional properties, the traditionally fermented dairy products (TFDP) from camel, mare, cow gained universal praise during long history of production. In this study, physicochemical composition microbial communities Khoormog, Chigee, Airag, TFDP Xilin Gol in China, were investigated compared. The analysis revealed a higher content total solid content, protein, fat Khoormog (12.5 ± 1.6%; 4.6 0.7%; 4.4 1.3%) compared Chigee (7.8 1.3%; 2.1...

By adding a small amount of HMPA approximately 2.5 equivalent or ca. 8.1 wt% H2 can be released from the LiAlH4–LiNH2 mixture at ambient temperature, which is ∼370 °C lower than corresponding solid-state dehydrogenation. NMR characterizations reveal formation intermediates, such as [AlH] and [AlN] species, during LiNH2 induces enrichment species in liquid phase further reacts with to produce H2, Li2NH Al.

Calcium amidoborane (Ca(NH2BH3)2, CaAB) is a new member of the borohydride family, which possesses interesting dehydrogenation features. CaAB also exhibits superior performance in chemoselective reduction. Our experimental results show that can reduce α,β-unsaturated carbonyl compounds chemoselectively to corresponding allylic alcohols at ambient temperature.

Abstract In this study, we report a new approach for the detection of ovine and caprine DNA in meat dairy products using real‐time PCR protocol. Our is based on use endogenous control species‐specific Taq Man fluorescence probes. With methodology, specifically detected products, with limits 0.001 ng 0.01 fresh processed meats, respectively, 0.00025 ng, 0.005 meat, milk, cheese, respectively. Artificial milk mixtures from sheep goat were used to validate results support that an efficient...

Abstract Natural fermentation of milk is a prerequisite in the production traditional dairy products and considered bioresource fermentative microorganisms probiotics. To understand microbial dynamics during distinct phases, roles different microbes, relationship between bacteria fungi, community was investigated by culture‐dependent culture‐independent approaches. Natural, static induces formation underlying curds superficial sour cream (Zuohe Mongolian language). From an overall...

Native chemical ligation (NCL) enables the total synthesis of proteins. However, poor peptide segment solubility remains a frequently encountered challenge. Here we introduce traceless linker that can be temporarily attached to Glu side chains overcome this problem. This strategy employs new tool, Fmoc-Glu(AlHx)-OH, which directly installed using standard Fmoc-based solid-phase synthesis. The incorporated residue, Glu(AlHx), is stable wide range protein conditions and removed through...

Abstract Mongolian cheese is not only a requisite source of food for the nomadic but also follows unique dairy artisanal method production, possessing high nutritional value and long shelf‐life. In this study, ancient technique production was investigated. The characterized by its high‐protein content (30.13 ± 2.99%) low‐fat (9.66 3.36%). Lactobacillus , Lactococcus, Dipodascus were predominant bacterial fungal genera, helveticus, Lactococcus piscium geotrichum species in cheese. microbiota...

Abstract A variety of secondary amines were obtained in high isolated yields the reductive amination aldehydes and ketones by using lithium amidoborane as reducing agent. Compared to ammonia borane, has higher reducibility, thus, exhibits faster reaction rate.

Multiple samples (117) of sweet sorghum stalk particles were analysed qualitatively and quantitatively during solid-state fermentation using near infrared spectroscopy chemometrics methods. The study indicated that the could be identified to lag phase, exponential phase or stationary ethanol process by discriminant analysis a principal component (PCA) model. first score PCA used predict extent reaction, regardless value parameters, such as pH sugar, water content. r 2 values multivariate...