A5051152936- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
National Institute of Allergy and Infectious Diseases
2018
National Institutes of Health
2018
Protective antibody responses to vaccination or infection depend on affinity maturation, a process by which high-affinity germinal center (GC) B cells are selected the basis of their ability bind, gather, and present antigen T follicular helper (Tfh) cells. Here, we show that human GC have intrinsically higher-affinity thresholds for both cell receptor (BCR) signaling gathering as compared with naïve these functions mediated distinct cellular structures pathways ultimately lead affinity- Tfh...
Abstract The selection of GC B cells that express high affinity cell receptors (BCRs) is driven by the ability to both signal through BCR and extract antigen present it follicular helper T (T FH cells). Using anti-kappa antibodies low (KD=3.9×10 −7 ) versus (KD=2.4×10 −9 affinities attached membranes as surrogate antigens, we show LZ are able discriminate between these responded only antigen. In contrast, naïve antigens. engaged membrane associated antigens highly dynamic F-actin ezrin-rich...