A5087387177- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Nanoparticle-Based Drug Delivery
- Metal complexes synthesis and properties
- Peptidase Inhibition and Analysis
- Nanoparticles: synthesis and applications
- Photodynamic Therapy Research Studies
- Nitric Oxide and Endothelin Effects
- Neuropeptides and Animal Physiology
- Porphyrin and Phthalocyanine Chemistry
- Renin-Angiotensin System Studies
- Carbohydrate Chemistry and Synthesis
- Nanoplatforms for cancer theranostics
- Organometallic Complex Synthesis and Catalysis
- Ferrocene Chemistry and Applications
- Graphene and Nanomaterials Applications
- Click Chemistry and Applications
- Glutathione Transferases and Polymorphisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Pulmonary Hypertension Research and Treatments
- Hormonal Regulation and Hypertension
- Analytical Chemistry and Sensors
- Enzyme Production and Characterization
- Protease and Inhibitor Mechanisms
- Porphyrin Metabolism and Disorders
University Hospital of Lausanne
2012-2021
University of Lausanne
2011-2021
University Hospitals Bristol NHS Foundation Trust
2013
St Michael’s Hospital
2013
St Michaels Hospital
2013
St Michael's Hospital
2013
École Polytechnique Fédérale de Lausanne
2005-2009
Weatherford College
2009
University of Neuchâtel
2000-2008
Roche (Switzerland)
2004
Water-soluble metalla-cages were used to deliver hydrophobic porphin molecules cancer cells. After internalization, the photosensitizer was photoactivated, significantly increasing cytotoxicity in During transport, remains nonreactive light, offering a new strategy tackle overall photosensitization, limitation often encountered photodynamic therapy.
Given the multiplicity of nanoparticles (NPs), there is a requirement to develop screening strategies evaluate their toxicity. Within EU-funded FP7 NanoTEST project, panel medically relevant NPs has been used alternative testing in medical diagnostics. As conventional toxicity tests cannot necessarily be directly applied same manner as for soluble chemicals and drugs, we determined extent interference with each assay process components. In this study, fully characterized NP suspensions...
A rationally designed Pt(IV) anticancer compound is described, employing the novel concept of tethering an inhibitor glutathione-S-transferase, enzyme associated with Pt-based drug-resistance, to cisplatin. Its inhibition activity, investigated using spectrophotometric and mass spectrometry-based techniques, cytotoxic profile in resistant cancer cells are described.
Five 5,10,15,20-tetra(4-pyridyl)porphyrin (TPP) areneruthenium(II) derivatives and a p-cymeneosmium two pentamethylcyclopentadienyliridium -rhodium analogues were prepared characterized as potential photosensitizing chemotherapeutic agents. The biological effects of all these assessed on human melanoma tumor cells, their cellular uptake intracellular localization determined. All molecules, except the rhodium complex which was not cytotoxic, demonstrated comparable cytotoxicity in absence...
Different types of NPs (nanoparticles) are currently under development for diagnostic and therapeutic applications in the biomedical field, yet our knowledge about their possible effects fate living cells is still limited. In present study, we examined cellular response human brain-derived endothelial to different size structure: uncoated oleic acid-coated iron oxide (8–9 nm core), fluorescent 25 50 silica NPs, TiO2 (21 mean core diameter) PLGA [poly(lactic-co-glycolic acid)]-PEO...
Angiotensin (Ang) II type 2 (AT2) receptor stimulation results in coronary vasodilation the rat heart. In contrast, AT2 receptor-mediated could not be observed large human arteries. We studied Ang II-induced of microarteries (HCMAs).HCMAs (diameter, 160 to 500 microm) were obtained from 49 heart valve donors (age, 3 65 years). constricted HCMAs, mounted Mulvany myographs, a concentration-dependent manner (pEC50, 8.6+/-0.2; maximal effect [E(max)], 79+/-13% contraction 100 mmol/L K+). The 1...
Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multiphoton microscopy. We present the first time complete survey of properties five nanomaterials (KNbO(3), LiNbO(3), BaTiO(3), KTP, and ZnO), describing their preparation stabilization providing quantitative estimations nonlinear response. In light prospective use biological clinical markers, we assess biocompatibility on human healthy cancerous cell lines. Finally, demonstrate...
Organometallic ruthenium(II) complexes of the general formula [Ru(η6-p-cymene)Cl2(L)] and [Ru(η6-p-cymene)Cl(L)2][BPh4] with modified phenoxazine- anthracene-based multidrug resistance (MDR) modulator ligands (L) have been synthesized, spectroscopically characterized, evaluated in vitro for their cytotoxic MDR reverting properties comparison free ligands. For an ligand, coordination to a arene fragment led significant improvement cytotoxicity as well Pgp inhibition activity. A similar, but...
Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) combined with magnetic resonance imaging (MRI) are under clinical evaluation to enhance detection of neurodegenerative diseases. A major improvement would be link therapeutic drugs the SPIONs achieve targeted drug delivery, either at cell surface or intracellularly, together active disease detection, without inducing reaction. Our objectives were define characteristics SPIONS able cell-specific interaction brain-derived structures. system...
Abstract Double trouble : A hybrid organic–inorganic (organometallic) inhibitor was designed to target glutathione transferases. The metal center is used direct protein binding, while the organic moiety acts as active‐site (see picture). mechanism of inhibition studied using a range biophysical and biochemical methods. magnified image
The expression and function in growth apoptosis of the renin–angiotensin system (RAS) was evaluated human glioblastoma. Renin angiotensinogen (AGT) mRNAs proteins were found by situ hybridisation immunohistochemistry glioblastoma cells. Angiotensinogen present cystic fluids. Thus, cells produce renin AGT secrete AGT. Human expressed renin, AGT, receptor, AT2 and/or AT1 determined RT–PCR Western blotting, respectively. RAS studied using culture. Angiotensinogen, des(Ang I)AGT,...
Ruthenium-arene complexes conjugated to ethacrynic acid were prepared as part of a strategy develop novel glutathione-S-transferase (GST) inhibitors with alternate modes activity through the organometallic fragment, ultimately provide targeted ruthenium-based anticancer drugs. Enzyme kinetics and electrospray mass spectrometry experiments using GST P1-1 its cysteine-modified mutant forms revealed that are effective enzyme inhibitors, but they also rapidly inactivate by covalent binding at...
In order to utilize macromolecules for drug targeting and delivery, a strategy tether organometallic ruthenium−arene drugs carrier protein molecules was developed. The approach involves the design of fragment capable conjugating linker on modified via hydrazone bond formation. proof-of-concept using recombinant human serum albumin is described.
Abstract The synthesis and in vitro anticancer activity of dihalogenido(η 6 ‐ p ‐cymene)(3,5,6‐bicyclophosphite‐α‐ D ‐glucofuranoside)ruthenium(II) complexes are described. compounds were characterized by NMR spectroscopy ESI mass spectrometry, the molecular structures dichlorido‐, dibromido‐ diiodido(η ‐cymene)(3,5,6‐bicyclophosphite‐1,2‐ O ‐isopropylidene‐α‐ determined X‐ray diffraction analysis. shown to undergo aquation first halido ligand aqueous solution, followed hydrolysis a PO bond...
Abstract Microglial cells react early to a neurotoxic insult. However, the bioactive factors and cell‐cell interactions leading microglial activation finally neuroprotective or neurodegenerative outcome remain be elucidated. Therefore, we analyzed reaction induced by methylmercury (MeHgCl) using cell cultures of different complexity. Isolated miroglia were found directly activated MeHgCl (10 −10 10 −6 M), as indicated process retraction, enhanced lectin staining, cluster formation. An...
A series of trans-platinum(IV) complexes with functionalized aromatic carboxylate ligands, cis,cis,trans-Pt(NH3)2Cl2(CO2C6H4R)2 (R = H (3), p-vinyl (4), p-methoxy (5), p-iodo (6), p-cyano (7), or o-carboxyl (8)) was synthesized and characterized by spectroscopic methods. Crystal structures 3, 4, 7, 8 were obtained, which revealed that their structural conformations influenced intramolecular H-bonding interactions. The evaluated for cellular uptake inhibition cell proliferation against a...