Marine Crenarchaeota are the most abundant single group of prokaryotes in ocean, but their physiology and role marine biogeochemical cycles unknown. Recently, a member this clade was isolated from sea aquarium shown to be capable nitrification, tentatively suggesting that may play oceanic nitrogen cycle. We enriched crenarchaeote North Sea water showed its abundance, not bacteria, correlates with ammonium oxidation nitrite. A time series study revealed abundance gene encoding for archaeal...

Chain elongation is an open-culture biotechnological process which converts volatile fatty acids (VFAs) into medium chain (MCFAs) using ethanol and other reduced substrates. The objective of this study was to investigate the quantitative effect CO2 loading rate on usages in a process. We supplied different rates continuously stirred anaerobic reactor, fed with propionate. Ethanol used upgrade itself caproate VFA (propionate) heptanoate. A high (2.5 LCO2·L–1·d–1) stimulated excessive...

The occurrence of anaerobic oxidation methane (AOM) and trace (TMO) was investigated in a freshwater natural gas source. Sediment samples were taken analyzed for potential electron acceptors coupled to AOM. Long-term incubations with (13)C-labeled CH4 ((13)CH4) different showed that both AOM TMO occurred. In most conditions, CO2 ((13)CO2) simultaneously increased formation, which is typical TMO. the presence nitrate, neither formation nor Net measured only sulfate as acceptor. Here, sulfide...

ABSTRACT Communities of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB) grow slowly, which limits the ability to perform physiological studies. High methane partial pressure was previously successfully applied stimulate growth, but it is not clear how different ANME subtypes associated SRB are affected by it. Here, we report on growth ANME-SRB in a membrane capsule bioreactor inoculated with Eckernförde Bay sediment that combines high-pressure incubation (10.1...

Membrane filtration systems are widely applied for the production of clean drinking water. However, accumulation particles on synthetic membranes leads to fouling. Biological fouling (i.e., biofouling) reverse osmosis and nanofiltration is difficult control by existing cleaning procedures. Improved strategies therefore needed. The bacterial diversity fouled has been studied, especially identify bacteria with specialized functions develop targeted approaches against these microbes. Previous...

Anaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the metabolism and occurrence of SAOB at extremely haloalkaline conditions were investigated, using highly enriched (M-SAO) sulfate-reducing (S-SAO) cultures from south-western Siberian...

The biogeography of eukaryotes in drinking water systems is poorly understood relative to that prokaryotes or viruses, limiting the understanding their role and management. A challenge with studying complex eukaryotic communities metagenomic analysis workflows are currently not as mature those focus on viruses. In this study, we benchmarked different strategies recover sequences genomes from data applied best-performing workflow explore factors affecting abundance diversity distribution...

A coupled-channels model for antinucleon-nucleon ($\overline{N}N$) scattering is presented. The $\overline{N}N$ channels are coupled to effective two-particle annihilation channels. neutron-proton mass difference and the Coulomb interaction taken exactly into account. This gives a detailed fit set of 977 data up ${T}_{\mathrm{lab}}=482$ MeV, with an overall $\frac{{\ensuremath{\chi}}^{2}}{\mathrm{data}}=1.39$. potentials found be long-ranged. discussion several experimental sets influence given.

Biological treatment processes have the potential to remove organic micropollutants (OMPs) during water treatment. The OMP removal capacity of conventional drinking such as rapid sand filters (RSFs), however, has not been studied in detail. We investigated and transformation product (TP) formation seven full-scale RSFs all treating surface water, using high-resolution mass spectrometry based quantitative suspect non-target screening (NTS). Additionally, we microbial communities with 16S rRNA...

Extensive geochemical data showed that significant methane oxidation activity exists in marine sediments. The organisms responsible for this are anaerobic methane-oxidizing archaea (ANME) occur consortia with sulfate-reducing bacteria. A distinct zonation of different clades ANME (ANME-1, ANME-2a/b, and ANME-2c) sediments, which could be related to the localized concentrations methane, sulfate, sulfide. In order test hypothesis we performed long-term incubation sediments under defined...

Summary Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled reduction Fe(III). The response G. electron donors acetate, formate, hydrogen and mixture all three with Fe(III) citrate as acceptor was studied using comparative physiological proteomic approaches. Variations in supplied resulted differential abundance proteins involved citric acid cycle (CAC), gluconeogenesis, transport, hydrogenases formate dehydrogenase. Our results provided new...

• AOP decreases the number and concentration of OMP chances negative impact on ecology groundwater quality. decreased concentrations in drinking water with no measurable effect quality parameters. MARR produces a highly stable chemical microbiological composition levels out seasonal peak fluctuations. There is redundancy removal by AOP, but able to remove certain that not, vice versa.

The concentrations of electron donors and acceptors in the terrestrial subsurface biosphere fluctuate due to migration mixing fluids, but mechanisms rates at which microbial communities respond these changes are largely unknown. Subsurface exhibit long cellular turnover times often considered relatively static-generating just enough ATP for maintenance. Here, we investigated how populations CH4 oxidizers acceptor availability by monitoring biological geochemical composition a 1339...

The COVID-19 pandemic has brought new momentum to 'wastewater-based epidemiology' (WBE). This approach can be applied monitor the levels of antibiotic-resistant genes (ARGs), which in terms are used make inferences about burden antimicrobial resistance (AMR) human settlements. However, there is still little information temporal variability ARG measured wastewater streams and how these influence made occurrence AMR communities. goal this study was hence gain insights into influent effluent...

Rapid sand filtration (RSF) is used during drinking water production for removal of particles, possible harmful microorganisms, organic material and inorganic compounds such as iron, manganese, ammonium methane. However, RSF can also be certain micropollutants (OMPs). In this study, it was investigated if OMP in columns packed with from full scale RSFs could stimulated by bioaugmentation (i.e. inoculating another RSF) and/or biostimulation addition nutrients, vitamins trace-elements that...

This study provides important new insights on how to achieve high sulfur selectivities and stable gas biodesulfurization process operation in the presence of both methanethiol H2S feed gas. On basis previous research, we hypothesized that a dual bioreactor lineup (with an added anaerobic bioreactor) would favor sulfur-oxidizing bacteria (SOB) yield higher selectivity. Therefore, focus present was enrich thiol-resistant SOB can withstand methanethiol, most prevalent toxic thiol...

Chemolithoautotrophic sulfur-oxidizing bacteria (SOB) are crucial key players in biotechnological processes to remove hydrogen sulfide from sour gas streams. Several different haloalkaliphilic SOB have been detected and isolated lab- full-scale facilities, which all performed differently considering end product yields (sulfur sulfate) conversion rates. Understanding regulating bacterial community dynamics biodesulfurization will enable optimization of the process operation. We developed...

Abstract The concentrations of electron donors and acceptors in the terrestrial subsurface biosphere fluctuate due to migration mixing fluids, but mechanisms rates at which microbial communities respond these changes are largely unknown. Subsurface exhibit long cellular turnover times often considered relatively static—generating just enough ATP for maintenance. Here, we investigated how populations CH 4 oxidizers acceptor availability by monitoring biological geochemical composition a 1,339...