- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Dengue and Mosquito Control Research
- Milk Quality and Mastitis in Dairy Cows
- Liver Disease and Transplantation
- Malaria Research and Control
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Hepatitis Viruses Studies and Epidemiology
- Identification and Quantification in Food
Tribhuvan University
2020-2025
The largest dengue outbreak in the history of Nepal occurred 2022, with a significant number casualties. It affected all 77 districts, nation's capital, Kathmandu (altitude 1300 m), being hardest hit. However, molecular epidemiology this outbreak, including virus (DENV) serotype(s) responsible for epidemic, remain unknown. Here, we report epidemic trends, clinico-laboratory features, and serotypes their viral load profiles that are associated Nepal. Dengue-suspected febrile patients were...
Hepatitis A virus (HAV) and hepatitis E (HEV) are thought to have similar routes of transmission epidemiology in developing countries. This study investigated the seroprevalences these pathogens among healthy individuals Dhanusha district Madesh Province, Nepal. Ninety serum samples collected between 2022 2023 were analyzed by enzyme-linked immunosorbent assay detect immunoglobulin (Ig)G antibodies against HAV HEV. Individuals (median age, 23 years; range, 1-45 years) sorted into five age...
In 2023, Nepal faced its second largest dengue outbreak ever, following a record-breaking number of cases in 2022, characterized by the expansion infections into areas higher altitudes. However, characteristics 2023 circulating virus (DENV) and vector density remain poorly understood. Therefore, we performed DENV serotyping, clinical laboratory assessment, entomological analysis central Nepal. A total 396 fever Dhading hospital suspected being positive were enrolled, blood samples collected...
The emergence of antibiotic resistance in microorganisms and the presence such isolates milk pose a great risk to public health. Therefore, this study aims determine susceptibility pattern Escherichia coli Staphylococcus aureus isolated from assess microbial quality milk. For this, total 70 samples were collected bacterial count (TBC) was determined. E. S. using their respective selective media while testing carried out by Kirby Bauer Disc Diffusion method. TBC showed that raw contained...