A5103518971- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Postharvest Quality and Shelf Life Management
- Muscle Physiology and Disorders
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Metabolism and Genetic Disorders
- Enzyme Production and Characterization
- Insect Resistance and Genetics
- Plant Physiology and Cultivation Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Kidney Stones and Urolithiasis Treatments
- Neurological diseases and metabolism
- Trace Elements in Health
- Clostridium difficile and Clostridium perfringens research
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Plant Micronutrient Interactions and Effects
- Microbial Metabolic Engineering and Bioproduction
- Single-cell and spatial transcriptomics
- Genomic variations and chromosomal abnormalities
University of Auckland
2015-2024
University of Cape Town
1993-2016
University of Liège
2002
Livestock Improvement Corporation
2002
Plant & Food Research
1998-2000
AgResearch
1997-1999
Cornell University
1999
We recently mapped a quantitative trait locus (QTL) with major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. subsequently exploited linkage disequilibrium to refine map position this QTL 3-cM interval bounded by microsatellite markers BULGE13 and BULGE09 . herein report positional candidate cloning QTL, involving (1) construction BAC contig spanning corresponding marker interval, (2) demonstration that very strong gene,...
Huntington's disease (HD) is an inherited autosomal dominant neurodegenerative disorder caused by expansion of a CAG trinucleotide repeat in the huntingtin (HTT) gene [Huntington's Disease Collaborative Research Group (1993) A novel containing that expanded and unstable on chromosomes. The Group. Cell, 72, 971–983]. Despite identification 1993, underlying life-long process effective treatments to prevent or delay it remain elusive. In effort fast-track treatment strategies for HD into...
The morphological and cytological changes which occurred in Clostridium acetobutylicum P262 during the production of acetone, butanol, ethanol an industrial fermentation medium were identified correlated with growth physiological changes. swollen, cigar-shaped clostridial forms involved conversion acids to neutral solvents, there was a correlation between number solvents. Sporulation mutants unable form stages ( cls mutants) did not produce Oligosporogenous showed reduced stage formation...
Significance We present evidence for the presymptomatic dysregulation of urea metabolism in Huntington’s disease (HD). identified increased levels a transporter transcript and other osmotic regulators striatum our prodromal sheep model HD concomitant increase striatal cerebellar urea. Elevated was also detected brain tissue from postmortem cases, including cases with low-level cell loss, implying that is not just product end-stage cachexia. Disruption known to cause neurologic impairment...
Huntington's disease (HD) is a fatal neurodegenerative caused by genetic expansion of the CAG repeat region in huntingtin (HTT) gene. Studies HD mouse models have shown that artificial miRNAs can reduce mutant HTT, but evidence for their effectiveness and safety larger animals lacking. transgenic sheep express full-length human HTT with 73 repeats. AAV9 was used to deliver unilaterally striatum an miRNA targeting exon 48 mRNA under control two alternative promoters: U6 or CβA. The treatment...
Traditional RNA extraction methods rely on the use of hazardous chemicals such as phenol, chloroform, guanidinium thiocyanate to disrupt cells and inactivate RNAse simultaneously. isolation from Caenorhabditis elegans presents another challenge due its tough cuticle, therefore several repeated freeze-thaw cycles may be needed cuticle before cell contents are released. In addition, a large number animals required for successful isolation. To overcome these issues, we have developed simple...
Huntington's disease (HD) is a neurodegenerative disorder caused by an expanded CAG repeat in exon 1 of the
Abstract Huntington’s disease (HD) is a dominantly inherited, progressive neurodegenerative disorder caused by CAG repeat expansion within exon 1 of HTT , encoding huntingtin. There are no therapies that can delay the progression this devastating disease. One feature HD may play critical role in its pathogenesis metabolic disruption. Consequently, we undertook comparative study metabolites our transgenic sheep model (OVT73). This does not display overt symptoms but has circadian rhythm...
Background: Huntington's disease is a neurodegenerative disorder, typically with clinical manifestations in adult years, caused by an expanded polyglutamine-coding repeat HTT. There are no treatments that delay or prevent the onset progression
Huntington's disease (HD) is a genetically-mediated neurodegenerative disorder wherein the aetiological defect mutation in gene (HTT), which alters structure of huntingtin protein (Htt) through lengthening its polyglutamine tract, thus initiating cascade that ultimately leads to premature death. However, neurodegeneration typically manifests HD only middle age, and mechanisms linking causative brain are poorly understood. Brain metabolism severely perturbed HD, some studies have indicated...
We are investigating the molecular basis of low temperature responses in apples, by identifying and characterising fruit genes which show altered expression response to cool‐storage, Two independent cold‐regulated clones (pAMTI pAMT2) were isolated from a cDNA library derived cool‐stored apple ( Malus domestics Borkh. cv. Granny Smith) fruit. These share only 27% amino acid identity with each other, but both high similarity plant metallothionein (MT)‐like proteins. The polypeptide encoded...
To examine whether enhanced diversity or numbers of oxalate-degrading bacteria in the gastrointestinal tracts black South Africans play a role determining rarity urolithiasis this group.Fresh faecal samples collected from healthy and white African male volunteers were analysed terms bacterial activity, relative species abundance. Varied populations prepared low-risk group showed significantly higher level oxalate degradation. Denaturing gradient gel electrophoresis analyses Lactobacillus...
Abstract Huntington’s disease (HD) is a neurodegenerative genetic disorder caused by an expansion in the CAG repeat tract of huntingtin (HTT) gene resulting behavioural, cognitive, and motor defects. Current knowledge pathogenesis remains incomplete, no course-modifying interventions are clinical use. We have previously reported development characterisation OVT73 transgenic sheep model HD. The 73 polyglutamine somatically stable therefore likely captures prodromal phase with absence...
Preharvest development and postharvest disappearance of watercore in `Fuji' apples ( Malus × domestica Borkh.) from a northern (Hawke's Bay, latitude 39° south) southern (Otago, 45° region New Zealand were compared. A new method for quantifying was developed. photocopy taken the symptoms after each fruit cut half through equator, then area affected flesh (photocopies black) measured using morphometric methods compared to unaffected white). Watercore more severe developed earlier season Otago...
Plasmid pMET7C containing a 6.05 kb DNA insert from Clostridium acetobutylicum P262 made Escherichia coli F19 cells sensitive to metronidazole. The nucleotide sequence of the C. controlling metronidazole sensitivity in E. revealed an ORF 972 bp which encoded protein 324 amino acids with calculated M r 35000. acid by contained helix-turn-helix DNA-binding domain and was homologous catabolite control protein, CcpA, Bacillus subtilis megaterium, tRNA repressor shl gene, GaIR, LaCI PurR...