A5077453280- Birth, Development, and Health
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Telomeres, Telomerase, and Senescence
- Peptidase Inhibition and Analysis
- Education Systems and Policy
- Obesity, Physical Activity, Diet
- Genetics, Aging, and Longevity in Model Organisms
- Autoimmune Bullous Skin Diseases
- Congenital heart defects research
- Vasculitis and related conditions
- Bacterial Genetics and Biotechnology
- Insect Resistance and Genetics
- Early Childhood Education and Development
- Enzyme Structure and Function
- Genetic Associations and Epidemiology
- Child Nutrition and Water Access
- Bone Metabolism and Diseases
- Genetic Neurodegenerative Diseases
- Mercury impact and mitigation studies
- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Bone and Joint Diseases
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
University of Auckland
2013-2024
Maurice Wilkins Centre
2013
Hospital for Special Surgery
2006
Traditional RNA extraction methods rely on the use of hazardous chemicals such as phenol, chloroform, guanidinium thiocyanate to disrupt cells and inactivate RNAse simultaneously. isolation from Caenorhabditis elegans presents another challenge due its tough cuticle, therefore several repeated freeze-thaw cycles may be needed cuticle before cell contents are released. In addition, a large number animals required for successful isolation. To overcome these issues, we have developed simple...
Wear debris challenge of macrophages provokes the generation proinflammatory cytokines, which contribute to periprosthetic osteolysis. However, it is not known whether this effect accompanied by reprogramming other cytokines present within tissue that may be involved in anti-osteoclastogenic activities. In study, we examined ability wear particles inhibit signaling two such interleukin-6 and interferon-gamma.Human osteoclast precursor cells were challenged with titanium or...
Abstract Telomeres are repetitive DNA sequences at the end of chromosomes that function to protect from degradation. Throughout life course, telomere length decreases with age and is influenced by environmental factors health conditions. This study aimed determine relative lengths in a diverse cohort about 4000 four-year-old children New Zealand. Linear regression was used investigate relationship between length, child gender, ethnicity, paternal deprivation. We observed substantial...
Background and Purpose Receptor activity‐modifying proteins ( RAMP s) define the pharmacology of calcitonin receptor‐like receptor CLR ). The interactions different s with this class B GPCR yield high‐affinity gene‐related peptide CGRP ) or adrenomedullin AM receptors. However, mechanism for is unclear. Experimental Approach Guided by models, we mutated residues in N ‐terminal helix , RAMP2 RAMP3 hypothesized to be involved interactions. These were assayed cAMP production AM2 together their...
Abstract Several early childhood obesity prediction models have been developed, but none for New Zealand's diverse population. We aimed to develop and validate a model predicting in 4–5-year-old Zealand children, using parental infant data from the Growing Up (GUiNZ) cohort. Obesity was defined as body mass index (BMI) age sex ≥ 95th percentile. Data on GUiNZ children were used derivation (n = 1731) internal validation 713). External performed Prevention of Overweight Infancy Study (POI, n...
Racism is a determinant of individual and offspring health. Accelerated telomere shortening, an indicator cellular aging, potential mechanism through which parental experience racism could affect offspring. Here we longitudinally evaluated the relationship between maternal lifetime ethnically-motivated verbal or physical attack, as reported in pregnancy, with length 4.5-year-old children. We also explored association positive feelings about one's culture length. Data come from nationally...
ABSTRACT Brain dopamine-serotonin vesicular transport disease is a rare caused by autosomal recessive mutations in the SLC18A2 gene, which encodes VMAT2 protein. membrane protein responsible for of monoamines, and its disruption negatively affects neurotransmission. This results severe neurodevelopmental disorder affecting motor skills development, causes muscular hypotonia. The condition was initially described consanguineous Saudi Arabian family with affected siblings homozygous P387L...
During glycolysis, yeast generates methylglyoxal (MG), a toxic metabolite that affects growth. Detoxification can occur when glyoxylase I (GLO1) and II (GLO2) convert MG to lactic acid. We have identified an additional, previously unrecognized role for GLO1 in sulfur assimilation the Saccharomyces cerevisiae. screening putative carbon-sulfur lyases, glo1 deletion strain showed significant production of H2S during fermentation. The also assimilated sulfate inefficiently but grew normally on...
Students measure the changes in cyclic AMP, a second messenger molecule, with light-based assay.
The central component AcrB of the Escherichia coli drug efflux complex AcrA–AcrB–TolC has been extensively investigated by X‐ray crystallography detergent–protein 3‐D crystals. In these crystals, packs as trimers – functional unit. We visualized AcrB–AcrB interaction in its native environment examining E. lipid reconstituted 2‐D which were overwhelmingly formed asymmetric stabilized strongly‐interacting monomers from adjacent trimers. Most interestingly, we observed lattices an arrangement...