Calcifying cystic odontogenic tumors (CCOTs) are benign that form abnormally keratinized ghost cells. Mutations in CTNNB1, which encodes beta-catenin, have been implicated the development of these tumors, but a causal relationship has not definitively established. Thus, mutational hot spots 50 cancer genes were examined by targeted next-generation sequencing 11 samples CCOT. other genes, observed 10 cases. These mutations constitutively activate beta-catenin signaling abolishing...

Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, bovine PDPN have already been established; however, mAbs pig (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), hybridomas producing pPDPN identified by flow cytometric screening. One...

Monoclonal antibodies (mAbs) against not only human, mouse, and rat but also rabbit, dog, cat, bovine, pig, horse podoplanins (PDPNs) have been established in our previous studies. PDPN is used as a lymphatic endothelial cell marker pathological diagnoses. However, mAbs Tasmanian devil (tasPDPN), which are useful for immunohistochemical analysis, remain to be developed. Herein, mice were immunized with tasPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/tasPDPN) cells, hybridomas...

CD133, also known as prominin-1, was first described a cell surface marker on early progenitor and hematopoietic stem cells. It is five-domain transmembrane protein composed of an N-terminal extracellular tail, two small cytoplasmic loops, large loops containing seven potential glycosylation sites, short C-terminal intracellular tail. CD133 has been used to identify cancer cells derived from primary solid tumors prognostic gliomas. Herein, we developed novel anti-CD133 monoclonal antibodies...

Abstract Background Oral lichen planus (OLP) is a chronic inflammatory oral mucosa disease that recognized as an potentially malignant disorder. However, the nature of OLP remains unclear. Methods We designed this study to examine demographic and clinical characteristics patients with evaluate associated transformation rate. A total 565 histopathological diagnosis who presented at our department between 2001 2017 were retrospectively studied. Patients had features lichenoid lesions (OLLs)...

Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse rat rabbit dog bovine have been established, anticat (cPDPN) mAbs not developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing...

Abstract The aims of this study were to evaluate in vivo the biological responses implants composed biodegradable anodized WE43 (containing magnesium yttrium, rare earth elements and zirconium; Elektron SynerMag®) alloy, monolithic alloy poly‐ l ‐lactic acid (PLLA), which are commonly used materials clinic settings, effectiveness as bone screws. osteosynthesis was evaluated using a fracture model involving tibia beagle dogs. For implants, radiological, histological evaluation revealed that...

Podoplanin (PDPN), also known as T1alpha, has been used a lung type I alveolar cell marker in the pathophysiological condition. Although we have established several monoclonal antibodies (mAbs) against mammalian PDPNs, mAbs tiger PDPN (tigPDPN), which are useful for immunohistochemical analysis, remain to be developed. In this study, immunized mice with tigPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/tigPDPN) and screened hybridomas producing tigPDPN using flow cytometry....

Recent studies have shown that Notch signaling is involved in many types of cancers, including oral squamous cell carcinomas (OSCCs). However, the role tumor microenvironment not yet fully understood. In this study, we investigated roles NOTCH3 cancer associated fibroblasts (CAFs) OSCCs. Immunohistochemical study 93 human tongue OSCC cases indicated about one third OSCCs showed expression CAFs, and significantly correlated with tumor-size. vitro lines, especially HO1-N-1 cells stimulated...

Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), hybridomas producing horPDPN screened using flow cytometry. One of mAbs,...

CD44 is widely expressed on the surface of most tissues and all hematopoietic cells, regulates many genes associated with cell adhesion, migration, proliferation, differentiation, survival. has also been studied as a therapeutic target in several cancers. Previously, an anti‑CD44 monoclonal antibody (mAb), C44Mab‑5 (IgG1, kappa) was established by immunizing mice CD44‑overexpressing Chinese hamster ovary (CHO)-K1 cells. recognized isoforms, showed high sensitivity for flow cytometry...

Abstract The distribution of cells expressing SARS‐CoV‐2 entry factor angiotensin‐converting enzyme 2 (ACE2) and transmembrane serine protease (TMPRSS2) in human oral tissues were tested. investigation was conducted with normal flesh tissue paraffin‐embedded specimens. ACE2 TMPRSS2 expression detected all subjects the mucosa keratinized stratified squamous epithelia tongue non‐keratinized lip cheek. It found that is expressed cytoplasm on cell membrane mainly stratum granulosum while...

The development of protein-specific antibodies is essential for understanding a wide variety biological phenomena. Parasitic and viral infections cancers are known to occur within California sea lion (Zalophus californianus) populations. However, sensitive specific monoclonal (mAbs) the pathophysiological analysis tissues have not yet been developed. A type I transmembrane glycoprotein, podoplanin (PDPN), diagnostic marker lymphatic endothelial cells. We previously developed several...

Podocalyxin (PODXL) overexpression is associated with progression, metastasis, and poor outcomes in cancers. We recently produced the novel anti-PODXL monoclonal antibody (mAb) PcMab-47 (IgG1, kappa). Herein, we engineered into 47-mG2a, a mouse IgG2a-type mAb, to add antibody-dependent cellular cytotoxicity (ADCC). further developed 47-mG2a-f, core fucose-deficient type of 47-mG2a augment its ADCC. Immunohistochemical analysis oral cancer tissues using revealed that latter stained squamous...

The multicellular spheroid model partly mimics tumor microenvironments in vivo and has been reported plenty of studies regarding radiosensitivity. However, clear isolation quiescent proliferating cells live conditions quite difficult owing to technical limitations; therefore, comprehensive characterization could not be done thus far. In this study, we succeeded separately isolating different cell types using a fluorescent ubiquitination‐based cycle indicator (Fucci) determining their...

Podoplanin (PDPN) has been utilized as a lymphatic endothelial cell marker especially in pathological diagnoses. Therefore, sensitive and specific monoclonal antibodies (mAbs) targeting PDPN are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues. Recently, anti-PDPN mAbs against many species, such human, mouse, rat, rabbit, dog, cat, bovine, pig, horse were established our studies. However, anticetacean (whale) (wPDPN) not yet. In this study, we immunized...

Sensitive and specific monoclonal antibodies (mAbs) against not only human but also mouse, rat, rabbit, dog, cat, bovine, pig, horse podoplanins (PDPNs) have been established in our previous studies. However, anti-goat PDPN (gPDPN) has yet. utilized as a lymphatic endothelial cell marker especially pathological diagnoses; therefore, mAbs for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues are needed. Although we recently demonstrated that an anti-bovine mAb,...

Podoplanin (PDPN) is a type I transmembrane glycoprotein that expressed in normal tissues, including renal corpuscles and lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), screened hybridomas, which are producing anti-pPDPN mAbs. One of mAbs, PMab-210 (an IgG1,...

BRD4, a member of the bromodomain and extra-terminal domain (BET) protein family, plays role in organization super-enhancers transcriptional activation oncogenes cancer is recognized as promising target for therapy. microRNAs (miRNAs), endogenous small noncoding RNAs, cause mRNA degradation or inhibit translation their genes by binding to complementary sequences. miRNA mimics simultaneously targeting several tumor-promoting BRD4 may be useful therapeutic agents tumor-suppressive miRNAs...

HER3 belongs to the epidermal growth factor receptor (EGFR) family and is known form an active heterodimer with other three members EGFR, HER2, HER4. overexpressed in lung, breast, colon, prostate, gastric cancers. In present study, we developed validated anti‑HER3 monoclonal antibody (mAb), H3Mab‑17 (IgG2a, kappa), by immunizing mice HER3‑overexpressed CHO‑K1 cells (CHO/HER3). was found react specifically endogenous colorectal carcinoma cell lines, using flow cytometry. The KD for CHO/HER3...