A5033890369- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Protein Structure and Dynamics
- Lipid metabolism and biosynthesis
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- Heat shock proteins research
- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Dialysis and Renal Disease Management
- Nitric Oxide and Endothelin Effects
- Diabetes Management and Research
- Drug Transport and Resistance Mechanisms
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Paraoxonase enzyme and polymorphisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Redox biology and oxidative stress
- Cardiovascular Health and Disease Prevention
- Genetic Associations and Epidemiology
- Lipoproteins and Cardiovascular Health
- Computational Drug Discovery Methods
- Diet, Metabolism, and Disease
- Receptor Mechanisms and Signaling
University of Washington
2006-2023
Seattle University
2019
Mayo Clinic
2016
Mayo Clinic in Arizona
2016
Washington University in St. Louis
2006
Oklahoma State University
2000-2003
Oklahoma Medical Research Foundation
2000
Monocytes differentiate into heterogeneous populations of tissue macrophages and dendritic cells (DCs) that regulate inflammation immunity. Identifying specific myeloid in vivo is problematic, however, because only a limited number proteins have been used to assign cellular phenotype. Using mass spectrometry bone marrow-derived cells, we provided global view the proteomes M-CSF-derived macrophages, classically alternatively activated GM-CSF-derived DCs. Remarkably, expression levels half...
Coronary endothelial dysfunction (ED)-an early marker of atherosclerosis-increases the risk cardiovascular events.We tested hypothesis that cholesterol efflux capacity and high-density lipoprotein (HDL) particle concentration predict coronary ED better than HDL-cholesterol (HDL-C).We studied 80 subjects with nonobstructive (<30% stenosis) artery disease. was defined as <50% change in blood flow response to intracoronary infusions acetylcholine during diagnostic angiography. Cholesterol HDL...
A subset of people with long-standing type 1 diabetes (T1D) appears to be protected from microvascular and macrovascular complications. Previous studies have focused on improved abilities respond glucose its downstream effects as protective mechanisms. It is unclear whether lipoproteins play a role in the vascular health these people. We therefore determined HDL particle concentration, size, function, and/or protein composition associate protection complications.We studied two independent...
Hsp90 and its co-chaperone Cdc37 facilitate the folding activation of numerous protein kinases. In this report, we examine structure−function relationships that regulate interaction with an Hsp90-dependent kinase, heme-regulated eIF2α kinase (HRI). Limited proteolysis native recombinant Cdc37, in conjunction MALDI-TOF mass spectrometry analysis peptide fragments microsequencing, indicates is comprised three discrete domains. The N-terminal domain (residues 1−126) interacts client HRI...
HDL is the primary mediator of cholesterol mobilization from periphery to liver via reverse transport (RCT). A critical first step in this process uptake lipid-loaded macrophages by HDL, a function inversely associated with prevalent and incident cardiovascular disease. We hypothesized that dynamic ability undergo remodeling exchange apoA-I an important potentially rate-limiting aspect RCT. In study, we investigated relationship between HDL-apoA-I (HAE) serum (HDL-C) efflux capacity....
Hsp90 and p50(cdc37) provide a poorly understood biochemical function essential to certain protein kinases, recent models describe as an exclusive hsp90 cohort which links machinery client kinases. We here the recovery of in immunoadsorptions directed against cohorts FKBP52, cyp40, p60HOP, hsp70, p23. Additionally, monoclonal antibodies FKBP52 coadsorb maturation intermediates hsp90-dependent kinases p56(lck) HRI, presence these significantly increases representation on FKPB52 machinery....
HDLs are nanoparticles with more than 80 associated proteins, phospholipids, cholesterol, and cholesteryl esters. The potential inverse relation of HDL to coronary artery disease (CAD) the effects on myriad other inflammatory conditions warrant a better understanding genetic basis proteome. We conducted comprehensive analysis regulation proteome isolated from panel 100 diverse inbred strains mice (the hybrid mouse diversity panel) examined protein composition efflux capacity identify novel...
Patients with chronic kidney disease (CKD) are at high risk for CVD. However, traditional CVD factors cannot completely explain the increased risk. Altered HDL proteome is linked incident in CKD patients, but it unclear whether other metrics associated this population. In current study, we analyzed samples from two independent prospective case-control cohorts of Clinical Phenotyping and Resource Biobank Core (CPROBE) Chronic Renal Insufficiency Cohort (CRIC). We measured particle sizes...
Most studies of protein oxidation have typically focused on the reactivity single amino acid side chains while ignoring potential importance adjacent sequences in directing reaction pathway. We previously showed that hypochlorous (HOCl), a specific product myeloperoxidase, inactivates matrilysin by modifying tryptophan and glycine (WG) residues catalytic domain. Here, we use model peptides mimic region involved this reaction, VVWGTA, VVWATA, library VVWXTA, to determine whether sequence...
Abstract High-density lipoproteins (HDL) are nanoparticles with >80 associated proteins, phospholipids, cholesterol and cholesteryl esters. A comprehensive genetic analysis of the regulation proteome HDL isolated from a panel 100 diverse inbred strains mice, Hybrid Mouse Diversity Panel (HMDP), revealed widely varied protein levels across strains. Some this variation was explained by local, cis-acting regulation, termed cis-protein quantitative trait loci. Variations in apolipoprotein...