Abstract Background Expression of matrix metalloproteases 2, 9 and 14 (MMP-2, MMP-9, MMP-14), tissue inhibitors metalloprotease 1 2 (TIMP-1, TIMP-2) vascular endothelial growth factor A (VEGF-A) is involved in tumor invasion metastasis via extracellular degradation angiogenesis. This study aimed to assess whether the expression MMP-2, MMP-14, TIMP-1, TIMP-2 tumors adjacent stroma associated with cervical cancer prognosis. Methods analyzed a retrospective cohort 64 patients. Protein was...

Behavioral risks such as age at first sexual intercourse, number of partners and partner's behavior are associated with an increased risk HPV infection, persistence the infection development neoplastic precursor lesions. The objective this study was to evaluate factors positivity a diagnosis cervical neoplasia in women referred abnormal smear.This evaluated series 198 smear. Risk for were investigated using questionnaire. All specimens tested 27 genotypes Roche polymerase chain reaction...

Vaginal infections may affect susceptibility to and clearance of human papillomavirus (HPV) infection chronic inflammation has been linked carcinogenesis. This study aimed evaluate the association between bacterial vaginosis (BV) inflammatory response (IR) with severity cervical neoplasia in HPV-infected women. HPV DNA was amplified using PGMY09/11 primers genotyping performed a reverse line blot hybridization assay 211 samples from women submitted excision transformation zone. The flora...

Background Human papillomavirus (HPV) infection, bacterial vaginosis (BV), and cervicitis may play a role in the presence of cytological abnormalities female adolescents young women. This study aimed to estimate prevalence HPV, BV women evaluate whether these conditions are associated with finding cervical smears. Methods Cervical smears were screened using conventional method HPV‐DNA detection was performed by PGMY‐PCR. defined as ≥20% clue cells Cervicitis assessed counting leukocytes five...

// Silvia Helena Rabelo-Santos 1 , Lara Termini 2 Enrique Boccardo 3 Sophie Derchain 4 Adhemar Longatto-Filho 5, 6 Maria Antonieta Andreoli 7 Cecília Costa Rafaella Almeida Lima Nunes Liliana Aparecida Lucci Ângelo-Andrade 8 Luisa Lina Villa 2, 9 and Luiz Carlos Zeferino School of Pharmacy, Federal University Goiás (UFG – Universidade de Goiás), Goiás, Brazil Innovation in Cancer Laboratory, Center Translational Research Oncology, Institute São Paulo (ICESP - Instituto do Câncer Estado...

Background Penile carcinoma (PC) is a rare, highly mutilating disease, common in developing countries. The evolution of penile cancer includes at least two independent carcinogenic pathways, related or unrelated to HPV infection. Objectives To estimate the prevalence, identify genotypes, and correlate with clinicopathological data on cancer. Methods A retrospective cohort study involving 183 patients PC undergoing treatment referral hospital Goiânia, Goiás, Midwestern Brazil, from 2003 2015....

Background . Approximately 90% of all anal cancers are associated with human papillomavirus (HPV), especially high-risk genotypes such as HPVs 16 and 18. Objective To investigate the clinical prognostic aspects presence, well genotypic distribution (HPV). Methods A retrospective study carried out over a 10-year period, using molecular data, PCR analysis reverse hybridization (INNO-LIPA kit), in cancers. The data was done descriptive univariate statistics, survival curves were made...

A study was carried out in 135 women of reproductive age to evaluate the role Papanicolaou smear performed cervical and vaginal samples for diagnosis bacterial vaginosis (BV), validate method this reproducibility criterion used. The smears were stained using evaluated by two separate observers. finding 20% or more clue cells considered positive presence BV. This diagnostic presented a sensitivity 87%, specificity 94%, predictive value 81%, negative 96%, both samples. There excellent...

Abstract High‐risk human papillomavirus (HPV) is considered the main etiological agent for cervical neoplasia. However, presence of a single type HPV infection alone unlikely to be sufficient cause cancer. There epidemiologic evidence suggesting that and Chlamydia trachomatis play central role in etiology intraepithelial neoplasia subsequent To evaluate prevalence seropositivity C. women referred colposcopy clinic due an abnormal smear examine effect this association on severity Following...

Abstract Background Cervical cancer ranks third in prevalence and fourth as cause of death women worldwide. In Brazil, 17,540 were diagnosed 2012 with the disease. Persistent infection high-risk HPV types is a necessary condition for development pre-invasive invasive cervical neoplasia. Currently, over 100 have been identified, but HPV16 18 are recognized mayor culprits carcinogenesis. Our objective was to assess relationships between single- (ST) multiple-type (MT) infections patients’ age...

Abstract Alterations in specific DNA damage repair mechanisms the presence of human papillomavirus (HPV) infection have been described different experimental models. However, global effect HPV on expression genes involved these pathways has not analyzed detail. In present study, we compared profile 135 among primary keratinocytes (PHK), HPV-positive (SiHa and HeLa) HPV-negative (C33A) cervical cancer derived cell lines. We identified 9 which pattern distinguishes tumor lines from C33A....

Cervical cancer, caused by high oncogenic risk Human Papillomavirus (HPV) infection, continues to be a public health problem, mainly in developing countries. Using peptide phage display as tool identify potential molecular targets HPV associated tumors, we identified α-mannosidase, among other enriched sequences. This enzyme is expressed both tumor and inflammatory compartment of the microenvironment. Several studies experimental models have shown that its inhibition swainsonine (SW) led...

Abstract The objective of this study was to analyze the association between bacterial vaginosis (BV) and squamous intraepithelial lesion (SIL). Pap smears were analyzed verify presence BV SIL. One hundred ten women with SIL comprised group, while 110 no cytological abnormalities served as controls. similarly present in both groups: 18% 12% without Results also similar when grade taken into consideration. detected 16% low‐grade control a higher rate (33%) found among high‐grade comparison...

Abstract We assessed the detection of high‐risk human papillomavirus DNA (HPV‐DNA) in women examined by a second Pap smear due to atypical glandular cells (AGC) detected their screening smear. In 91 included study, was taken and HPV‐DNA test performed using Hybrid Capture II (HC II). The showed no abnormalities 28 (31%) cases, ACG 17 (19%) high‐grade squamous intraepithelial lesions (HSIL) or worse 24 (26%). HC positive 36% cases. Considering diagnosis, 87% with HSIL, 100% situ...

To analyse the correlation between cytomorphological criteria in smears with atypical glandular cells (AGC) or adenocarcinoma situ (AIS) and human papillomavirus (HPV) reflex test results different neoplastic histological diagnoses, particularly to distinguish squamous neoplasia.A series of 155 women abnormalities their conventional cervical was included: 106 AGC, 35 AGC associated high-grade intraepithelial lesion (HSIL) 14 AIS. Two reviewers evaluated hybrid capture II (HCII) performed all...

This study was designed to analyze whether specific human papillomavirus (HPV) genotypes may predict histologic outcomes in women with glandular abnormalities their cervical smears. Of the 160 included, 111 were diagnosed atypical cells, 35 had both cells and high-grade squamous intraepithelial lesions, whereas 14 AIS, 1 case associated lesions. All underwent colposcopic examinations biopsy performed 129/160 (80.6%). Thirty-one (19.3%) considered negative for neoplasia scheduled follow-up....

Human papillomavirus (HPV) infection is associated with the development of anogenital and head neck cancers. In recent years a potential role HPV in colorectal cancer (CRC) has been suggested.To investigate presence carcinomas to study p16INK4a as marker transcriptionally active infection. addition, correlation between these findings CRC prognostic factors.Case control 92 cases cancers, 75 controls normal tissue adjacent tumor, 30 precursor lesions, including polyps adenomas. Paraffinized...

This study aimed at evaluating whether human papillomavirus (HPV) groups and E6/E7 mRNA of HPV 16, 18, 31, 33, 45 are prognostic cervical intraepithelial neoplasia (CIN) 2 outcome in women with a smear showing low-grade squamous lesion (LSIL).This cohort included biopsy-confirmed CIN who were followed up for 12 months, colposcopy performed every three months.Women negative or low-risk status showed 100% regression. The regression rates the 12-month follow-up 69.4% alpha-9 versus 91.7% other...