A5047978114- Glycosylation and Glycoproteins Research
- Ultrasound and Hyperthermia Applications
- Ultrasound and Cavitation Phenomena
- Photoacoustic and Ultrasonic Imaging
- Reproductive tract infections research
- RNA modifications and cancer
- Ultrasound Imaging and Elastography
- Herpesvirus Infections and Treatments
- Cervical Cancer and HPV Research
- MicroRNA in disease regulation
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Protein Interaction Studies and Fluorescence Analysis
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Advanced Proteomics Techniques and Applications
- Surfactants and Colloidal Systems
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- Galectins and Cancer Biology
- RNA Research and Splicing
- Pain Management and Treatment
- Cancer-related molecular mechanisms research
- Eicosanoids and Hypertension Pharmacology
- Genomics and Phylogenetic Studies
Regeneron (United States)
2023
New York University
2014-2021
Westford Academy
2021
Biomedical Research Institute
2014-2020
Riverside Research Institute
2011-2015
Significance Carbohydrates (glycans) are complex cell surface molecules that control multiple aspects of biology, including cell–cell communication, cancer metastasis, and inflammation. Glycan biosynthesis requires the coordination many enzymes, but how this is regulated not well understood. Herein we show microRNA (miRNA), small noncoding RNA, a major regulator glycosylation. We map miRNA expression onto carbohydrate signatures obtained by using lectin microarrays, glycan analysis method....
Significance Influenza virus infection causes a range of outcomes from mild illness to death. The molecular mechanisms leading these differential host responses are currently unknown. Herein, we identify the induction high mannose, glycan epitope, as key mediator severe disease outcome. We propose mechanism in which activation unfolded protein response (UPR) upon influenza induces cell surface is then recognized by innate immune lectin MBL2, activating complement cascade and subsequent...
Significance Carbohydrates hold an unprecedented capacity for altering biological function, but determining which glycans and underlying enzymes are crucial a specific pathway is major impediment to our understanding of this posttranslational modification. Here we demonstrate that the mRNA target networks microRNA (miRNA), small noncoding RNA, identify glycosylation acting as regulatory elements within pathway. Leveraging miRNA-200 family (miR-200f), regulators epithelial-to-mesenchymal...
The cervicovaginal fluid (CVF) coating the vaginal epithelium is an important immunological mediator, providing a barrier to infection. Glycosylation of CVF proteins, such as mucins, IgG and S-IgA, plays critical role in their functions. Although multiple factors, hormones microflora, may influence glycosylation CVF, few studies have examined impact on this fluid. Herein we analyzed lavage (CVL) samples collected from 165 women under different hormonal conditions including: (1) no...
This two-part study investigated shell rupture of ultrasound contrast agents (UCAs) under static overpressure conditions and the subharmonic component from UCAs subjected to 20-MHz tonebursts. Five different polylactide-shelled with shell-thickness-to-radius ratios (STRRs) 7.5, 30, 40, 65, 100 nm/¿m were in a glycerol-filled test chamber. A video microscope imaged as pressure varied 2 330 kPa over 90 min. Images postprocessed obtain threshold for diameter individual microbubbles. Backscatter...
Glycosylated proteins (i.e., mucins, IgG) are important mediators of innate antiviral immunity in the vagina; however, our current knowledge role that glycan themselves play genital is relatively low. Herein, we evaluate relationship between and glycomic composition cervicovaginal lavage fluid (CVL) collected as part a Phase I clinical trial testing impact two distinct formulations antiretroviral drug dapivirine. Using lectin microarray technology, discovered formulation (hydrogel- versus...
B4GALT1 encodes β-1,4-galactosyltransferase 1, an enzyme that plays a major role in glycan synthesis the Golgi apparatus by catalyzing addition of terminal galactose. Studies increasingly suggest may be involved regulation lipid metabolism pathways. Recently, we discovered single-site missense variant Asn352Ser (N352S) functional domain Amish population, which decreases level LDL-cholesterol (LDL-c) as well protein levels ApoB, fibrinogen and IgG blood. To systematically evaluate effects...
Polymer-shelled ultrasound contrast agents (UCAs) may expel their encapsulated gas subject to ultrasound- induced shell buckling or rupture. Nonlinear oscillations of this bubble can produce a subharmonic component in the backscatter. This study investigated relationship between gas-release mechanism and shell-thickness- to-radius ratios (STRRs) polymer-shelled UCAs. Three types polylactide-shelled UCAs with STRRs 7.5, 40, 100 nm/μm were studied. Each UCA population had nominal mean diameter...
Glycosylation is a key component in the establishment of healthy symbiotic bacterial colonization patterns and involved maintenance mucosal barriers to infection, regulating both adaptive innate immunity. Changes glycosylation state have powerful effects on stabilization activation antibodies can alter microbicidal immune molecules. Thus glycome marker modulator We recently showed strong correlation between status cervicovaginal (CVL) fluid microbiome status, using lectin microarray...
ABSTRACT Influenza virus infections cause a wide variety of outcomes, from mild disease to 3-5 million cases severe illness and ~290,000-645,000 deaths annually worldwide. The molecular mechanisms underlying these disparate outcomes are currently unknown. Glycosylation within the human host plays critical role in influenza biology. However, impact modifications have on severity has not been examined. Herein, we profile glycomic responses infection as function using ferret model our lectin...
Glycan biosynthesis is a complex and highly non‐linear process requiring large number of diverse proteins (glycosyltransferases, glycosidases sugar nucleotide transporters) to act in accord. An increasing recent reports, including previous work from our lab, suggest possible role microRNAs (miRNAs), small non‐coding RNA that complement the 3’ or 5’ untranslated region (UTR) mRNA attenuate translation, regulation overall glycan expression (glycome) via post‐transcriptional related gene...
Cell surface glycans create an intricate source of biological information which is dynamically altered by cells in developmental processes and used pathogens during host invasion. Mapping the glycocode identification critical glycan altering enzymes confounded complexity redundancy biosynthetic machinery. Recently, we integrated microRNA (miRNA) expression data with our glycomics analysis for NCI‐60 cancer cell line panel, showed that miRNA act as a major regulator human glycome targeting...
Polymer-shelled ultrasound contrast agents (UCAs) can undergo a "compression only" behavior leading to shell rupture and nonlinear response of the released gas bubbles when ultrasonically excited below 10 MHz. This study investigated if polymer-shelled UCAs exhibited similar at frequencies above Accordingly, two experiments were performed: one examined compression-induced UCA shells by subjecting them static overpressure, other subharmonic components in backscattered signal produced...
Polymer-shelled ultrasound contrast agents (UCAs) can undergo a “compression only” behavior leading to shell rupture and nonlinear response of the released gas bubbles when excited below 10 MHz. This study investigated if polymer-shelled UCAs exhibited similar at frequencies above Four varieties polylactide-shelled UCAs, each with distinct shell-thickness-to-radius ratio (STRR), were employed; STRRs 7.5, 40, 65, 100 nm/μm. Two experiments performed: one examined compression-induced UCA...
This study investigated the behavior of polymer-shelled ultrasound contrast agents (UCAs) different shell-thickness-to-radius ratios (STRRs) when subjected to static overpressure. Three varieties polylactide shelled UCAs with STRRs 7.5, 40 and 100 nm/μm were studied. A custom-designed pressure chamber was employed observe through a video microscope. normalized cross-sectional area individual obtained from binarized UCA images. Integration over time, defined as Buckling factor (Bf), provided...
Schizophrenia (SCZ) is one of the most prevalent psychotic disorder that affected an estimated 20 million worldwide, according to WHO estimates in 2019. It characterized by positive symptoms such as delusions and hallucinations well negative reduced speech apathy. While many factors, including genetic, epigenetic, environmental, chemical imbalances have been thought be linked with this disease, pathogenesis SCZ still remains elusive without much mechanistic insights. Many functional studies...