A5044671693- Influenza Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Respiratory viral infections research
- interferon and immune responses
- Mosquito-borne diseases and control
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Infectious Encephalopathies and Encephalitis
- Parkinson's Disease Mechanisms and Treatments
- Viral Infections and Outbreaks Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Autism Spectrum Disorder Research
- Mitochondrial Function and Pathology
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Inflammasome and immune disorders
- Tryptophan and brain disorders
- Nerve injury and regeneration
- Advanced Glycation End Products research
- Nuclear Receptors and Signaling
Rutgers, The State University of New Jersey
2020-2025
Rutgers Sexual and Reproductive Health and Rights
2023-2024
New York University
2017-2023
Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting controlling outbreaks is the timely accurate characterization emerging virus strains. We present portable microfluidic platform containing carbon nanotube arrays with differential filtration porosity rapid enrichment optical identification viruses. Different strains (or unknown viruses) can be enriched identified real time through multivirus capture component conjunction...
Abstract Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the death of midbrain dopamine neurons. The pathogenesis PD poorly understood, though misfolded and/or aggregated forms protein α-synuclein have been implicated in several processes, including neuroinflammation and astrocyte activation. Astrocytes play complex roles during PD, initiating both harmful protective processes that vary over course disease. However, despite their significant regulatory...
Significance Influenza virus infection causes a range of outcomes from mild illness to death. The molecular mechanisms leading these differential host responses are currently unknown. Herein, we identify the induction high mannose, glycan epitope, as key mediator severe disease outcome. We propose mechanism in which activation unfolded protein response (UPR) upon influenza induces cell surface is then recognized by innate immune lectin MBL2, activating complement cascade and subsequent...
Virus and host factors contribute to cell-to-cell variation in viral infections determine the outcome of overall infection. However, extent variability at single-cell level how it impacts virus-host interactions a system are not well understood. To characterize dynamics transcription responses, we used RNA sequencing quantify multiple time points transcriptomes human A549 cells primary bronchial epithelial infected with influenza A virus. We observed substantial between cells, including...
Astrocyte activation is a common feature of neurodegenerative diseases. However, the ways in which dying neurons influence activity astrocytes poorly understood. Receptor interacting protein kinase-3 (RIPK3) signaling has recently been described as key regulator neuroinflammation, but whether this kinase mediates astrocytic responsiveness to neuronal death not yet studied. Here, we used 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model Parkinson's disease show that RIPK3 drives...
RNA viruses can exchange genetic material during coinfection, an interaction that creates novel strains with implications for viral evolution and public health. Influenza A occur when genome segments from distinct reassort in coinfected cells. Predicting potential genomic reassortment between influenza has been a long-standing goal. Experimental coinfection studies have shed light on factors limit or promote reassortment. However, determining the diverse remained elusive. To address this...
Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe, intermittently cause pandemics. Sequence information from virus genomes is instrumental in determining mechanisms underpinning antigenic evolution antiviral resistance. However, due to sequence diversity dynamics evolution, rapid high-throughput sequencing remains a challenge. We developed single-reaction A/B (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method amplifies most critical...
Abstract Background Zika virus (ZIKV) is an emerging flavivirus of global concern. ZIKV infection the central nervous system has been linked to a variety clinical syndromes, including microcephaly in fetuses and rare but serious neurologic disease adults. However, potential for influence brain physiology host behavior following apparently mild or subclinical less well understood. Furthermore, though deficits cognitive function are well-documented after recovery from neuroinvasive viral...
Influenza D virus (IDV) is a new type of influenza that uses cattle as the primary reservoir and infects multiple agricultural animals. Increased outbreaks in pigs serological genetic evidence human infection have raised concerns about potential IDV adaptation humans. Here, we developed plasmid-based reverse-genetics system can generate infectious viruses with replication kinetics similar to those wild-type following transfection cultured cells. Further characterization demonstrated rescued...
Innate immune signaling in the central nervous system (CNS) exhibits many remarkable specializations that vary across cell types and CNS regions. In setting of neuroinvasive flavivirus infection, neurons employ immunologic kinase receptor-interacting 3 (RIPK3) to promote an antiviral transcriptional program, independently traditional function this enzyme promoting necroptotic death. However, while recent work has established roles for neuronal RIPK3 controlling mosquito-borne infections,...
Summary While recent work has identified roles for immune mediators in the regulation of neural activity, capacity cell intrinsic innate signaling within neurons to influence neurotransmission remains poorly understood. However, existing evidence linking with neuronal function suggests that modulation may serve previously undefined host protection during infection central nervous system. Here, we identify a specialized RIPK3, kinase traditionally associated necroptotic death, preserving...
Influenza A virus, with the limited coding capacity of 10-14 proteins, requires host cellular machinery for many aspects its life cycle. Knowledge these cell requirements not only reveals molecular pathways exploited by virus or triggered immune system, but also provides further targets antiviral drug development. To uncover novel and key influenza infection, we assembled a large amount data from 12 cell-based gene-expression studies infection an integrative network analysis. We...
ABSTRACT Virus and host factors contribute to cell-to-cell variation in viral infections determine the outcome of overall infection. However, extent variability at single cell level how it impacts virus-host interactions a systems are not well understood. To characterize dynamics transcription responses, we used single-cell RNA sequencing quantify multiple time points transcriptomes human A549 cells primary bronchial epithelial infected with influenza A virus. We observed substantial between...
Astrocyte activation is a common feature of neurodegenerative diseases. However, the ways in which dying neurons influence activity astrocytes poorly understood. RIPK3 signaling has recently been described as key regulator neuroinflammation, but whether this kinase mediates astrocytic responsiveness to neuronal death not yet studied. Here, we used MPTP model Parkinson's disease show that drives dopaminergic cell and axon damage. Transcriptomic profiling revealed promoted gene expression...
Abstract Flaviviruses pose a significant threat to public health due their ability infect the central nervous system (CNS) and cause severe neurologic disease. Astrocytes play crucial role in pathogenesis of flavivirus encephalitis through maintenance blood-brain barrier (BBB) integrity modulation immune cell recruitment activation within CNS. We have previously shown that receptor interacting protein kinase-3 (RIPK3) is coordinator neuroinflammation during CNS viral infection, function...
ABSTRACT Influenza virus infections cause a wide variety of outcomes, from mild disease to 3-5 million cases severe illness and ~290,000-645,000 deaths annually worldwide. The molecular mechanisms underlying these disparate outcomes are currently unknown. Glycosylation within the human host plays critical role in influenza biology. However, impact modifications have on severity has not been examined. Herein, we profile glycomic responses infection as function using ferret model our lectin...
Abstract RNA viruses can exchange genetic material during coinfection, an interaction that creates novel strains with implications for viral evolution and public health. Influenza A occurs when genome segments from distinct reassort in coinfection. Predicting potential reassortment between influenza is a longstanding goal. Experimental coinfection studies have shed light on factors limit or promote reassortment. However, determining the diverse has remained elusive To fill this gap, we...
Innate immune signaling in the central nervous system (CNS) exhibits many remarkable specializations that vary across cell types and CNS regions. In setting of neuroinvasive flavivirus infection, neurons employ immunologic kinase receptor-interacting 3 (RIPK3) to promote an antiviral transcriptional program, independently traditional function this enzyme promoting necroptotic death. However, while recent work has established roles for neuronal RIPK3 controlling mosquito-borne infections,...
Abstract Parkinson’s disease (PD) is a neurodegenerative disorder characterized by death of midbrain dopamine neurons. The pathogenesis PD poorly understood, though misfolded and/or aggregated forms the protein α-synuclein have been implicated in several processes, including neuroinflammation and astrocyte activation. Astrocytes play complex roles during PD, initiating both harmful protective processes that vary over course disease. However, despite their significant regulatory...
Abstract Zika virus (ZIKV) is an emerging flavivirus of global concern. ZIKV infection the central nervous system has been linked to a variety clinical syndromes, including microcephaly in fetuses and rare but serious neurologic disease adults. However, potential for influence brain physiology host behavior following recovery from apparently mild or subclinical less well understood. Furthermore, though deficits cognitive function are well-documented neuroinvasive viral infection, impact on...