A5036945164- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Microbial infections and disease research
- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Livestock and Poultry Management
- Extracellular vesicles in disease
- Mycobacterium research and diagnosis
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Hepatitis B Virus Studies
- Animal Genetics and Reproduction
Pennsylvania State University
2016-2025
Guangxi University
2025
Shanghai Tenth People's Hospital
2021
Tongji University
2021
University of Hong Kong
2006
Preparedness for a possible influenza pandemic caused by highly pathogenic avian A subtype H5N1 has become global priority. The spread of the virus to Europe and continued human infection in Southeast Asia have heightened concern. It remains unknown from where strain may emerge; current attention is directed at Vietnam, Thailand, and, more recently, Indonesia China. Here, we report that genetically antigenically distinct sublineages established poultry different geographical regions Asia,...
Rapid and specific detection of avian influenza virus (AIV) is urgently needed due to the concerns over potential outbreaks highly pathogenic H5N1 in animals humans. Aptamers are artificial oligonucleic acids that can bind target molecules, show comparable affinity for viruses better thermal stability than monoclonal antibodies. The objective this research was use a DNA-aptamer as recognition element portable Surface Plasmon Resonance (SPR) biosensor rapid AIV poultry swab samples. A SPR...
Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting controlling outbreaks is the timely accurate characterization emerging virus strains. We present portable microfluidic platform containing carbon nanotube arrays with differential filtration porosity rapid enrichment optical identification viruses. Different strains (or unknown viruses) can be enriched identified real time through multivirus capture component conjunction...
Abstract Avian reovirus (ARV) infections of broiler and turkey flocks have caused significant clinical disease economic losses in Pennsylvania (PA) since 2011. Most the ARV-infected birds suffered from severe arthritis, tenosynovitis, pericarditis depressed growth or runting-stunting syndrome (RSS). A high morbidity (up to 20% 40%) was observed ARV-affected flock mortality occasionally as 10%. ARV turkeys were diagnosed for first time PA From 2011 2014, a total 301 isolations made affected...
Viruses pose serious infectious disease threats to humans and animals. To significantly decrease the mortality morbidity caused by virus infections, there is an urgent need of sensitive rapid point-of-care platforms for detection, especially in low-resource settings. Herein, we developed a smartphone-based platform highly selective detection avian influenza based on nanomaterial-enabled colorimetric detection. The 3D nanostructures, which serve as scaffold antibody conjugation capture virus,...
Rapid and simultaneous detection of multiple potential pathogens by portable devices can facilitate early diagnosis infectious diseases, allow for rapid effective implementation disease prevention treatment measures. The development a ZnO nanorod integrated microdevice as multiplex immunofluorescence platform highly sensitive selective avian influenza virus (AIV) is described. 3D morphology unique optical property the nanorods boost limit H5N2 AIV to low 3.6 × 103 EID50 mL-1 (EID50 : 50%...
Viral infectious diseases can erupt unpredictably, spread rapidly, and ravage mass populations. Although established methods, such as polymerase chain reaction, virus isolation, next-generation sequencing have been used to detect viruses, field samples with low count pose major challenges in surveillance discovery. We report a unique carbon nanotube size-tunable enrichment microdevice (CNT-STEM) that efficiently enriches concentrates viruses collected from samples. The channel sidewall the...
In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype avian influenza virus (AIV), used as an alternative reagent monoclonal antibodies in impedance biosensor utilizing microfluidics flow cell and interdigitated microelectrode for detection AIV. The gold surface embedded modified using streptavidin. biotinylated aptamer then immobilized on electrode biotin–streptavidin binding....
Significance A large Raman dataset collected on a variety of viruses enables the training machine learning (ML) models capable highly accurate and sensitive virus identification. The trained ML can then be integrated with portable device to provide real-time detection identification capability. We validate this conceptual framework by presenting type subtype results using convolutional neural network classify spectra viruses. interpretable model developed for presents promising potential in...
The survival or clearance of the avian influenza virus (AIV) subtype H7N2 in its chicken host was evaluated using experimentally infected specific pathogen free (SPF) chickens different age groups. Birds ages were successfully with infectious doses ranging between 104.7 and 105.7 ELD50 per bird. In birds, infective undetectable usually by third week following exposure. infectivity inactivation time AIV various environmental conditions studied manure, heat, ethanol, pH, disinfectants....
In recent years, the infection rate of avian reovirus (ARV) in chicks has been continuously increasing, seriously endangering healthy development China's chicken farming industry. However, pathogenicity this virus immune organs less studied. study, an ARV strain (Chicken/XJ/China/2022/01/16) was isolated from arthritic broiler Xinjiang province and whole genome sequencing performed using next generation (NGS) technology, sequence analysis showed that a type V variant gene. order to further...
Nephropathogenic infectious bronchitis (NIB) was diagnosed in 28 virus (IBV)–vaccinated commercial chicken flocks Pennsylvania from December 1997 to July 2000. Early clinical signs were increased flock mortality and urinary water loss (polyuria pollakiuria) leading wet litter. Daily ranged 0.01% layers 2.45% broilers, with total broiler as high 23%. Severe renal swelling accumulation of urates the tubules commonly seen. Visceral gout urolithiasis less frequently observed. Histopathologic...
An outbreak of H7N2 low-pathogenicity (LP) avian influenza (AI) occurred in a two-county area Pennsylvania from December 1996 through April 1998. The resulted infection 2,623,116 commercial birds on 25 premises encompassing 47 flocks. Twenty-one (one premise with twice) the twenty-five infected housed egg-laying chickens and one each had turkeys, layer pullets, quail, mixed backyard dealer flock. Despite close proximity flocks to broiler flocks, no broilers were identified. Experimentally,...
Abstract Using next-generation sequencing (NGS) for full genomic characterization studies of the newly emerging avian orthoreovirus (ARV) field strains isolated in Pennsylvania poultry, we identified two co-infection ARV variant from one isolate obtained ARV-affected young layer chickens. The de novo assembly reads generated 19 contigs different according to 10 genome segments each strain. variants had same M2 segment. complete genomes were 23,493 bp length and dsRNA ranged 1192 (S4) 3958...
Viral diseases are perpetual threats to human and animal health. Detection characterization of viral pathogens require accurate, sensitive, rapid diagnostic assays. For field clinical samples, the sample preparation procedures limit ultimate performance utility overall virus protocols. This study presents development a microfluidic device embedded with porous silicon nanowire (pSiNW) forest for label‐free size‐based point‐of‐care capture in continuous curved flow design. The pSiNW forests...
Recent discoveries reveal that extracellular vesicles (EVs) play an important role in transmitting signals. Although this emerging transcellular pathway enables a better understanding of neural communication, the lack techniques for effectively isolating EVs impedes their studies. Herein, we report emergent high-throughput platform consisting three-dimensional carbon nanotube arrays rapidly capture different based on sizes, without any labels. More importantly, label-free maintains integrity...