A5051809547- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Microscopic Colitis
- Animal Virus Infections Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Reproductive tract infections research
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Cervical Cancer and HPV Research
- Syphilis Diagnosis and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Bacterial Infections and Vaccines
Penn State Milton S. Hershey Medical Center
2013-2024
Pennsylvania State University
2004-2022
Augusta University
2007
Yale University
2007
VA Connecticut Healthcare System
2007
Children's National
2007
Washington University Medical Center
2007
George Washington University
2007
Cleveland Clinic
2007
Providence Hospital
2007
ABSTRACT The performance of the new ePlex Respiratory Pathogen (RP) panel (GenMark Diagnostics) for simultaneous detection 19 viruses (influenza A virus; influenza H1 2009 H3 B adenovirus; coronaviruses [HKU1, OC43, NL63, and 229E]; human rhinovirus/enterovirus; metapneumovirus; parainfluenza 1, 2, 3, 4; respiratory syncytial virus [RSV] [RSV subtype RSV B]) 2 bacteria ( Mycoplasma pneumoniae Chlamydia ) was evaluated. Prospectively retrospectively collected nasopharyngeal swab (NPS)...
Significance A large Raman dataset collected on a variety of viruses enables the training machine learning (ML) models capable highly accurate and sensitive virus identification. The trained ML can then be integrated with portable device to provide real-time detection identification capability. We validate this conceptual framework by presenting type subtype results using convolutional neural network classify spectra viruses. interpretable model developed for presents promising potential in...
Quantification of HIV RNA in plasma is critical for identifying the disease progression and monitoring effectiveness antiretroviral therapy. While RT-qPCR has been gold standard viral load quantification, digital assays could provide an alternative calibration-free absolute quantification method. Here, we reported a Self-digitization Through Automated Membrane-based Partitioning (STAMP) method to digitalize CRISPR-Cas13 assay (dCRISPR) amplification-free HIV-1 RNAs. The Cas13 was designed,...
ABSTRACT Clostridium difficile , the primary cause of nosocomial diarrhea in United States and many other industrialized countries, is recognized as a major health concern because its ability to severe intestinal disease leading complications such relapses infections due vancomycin-resistant enterococci. The results from two toxins, toxins A B, produced by this pathogen. In study, we evaluated TOX A/B TEST, new 1-h enzyme immunoassay (EIA) that detects B. We compared test with tissue culture...
Probiotics have beneficial effects in rodent models of Clostridium difficile (C. diffiicle)-induced colitis. The spore forming probiotic strain Bacillus Coagulans GBI-30, 6086 (BC30) has demonstrated anti-inflammatory and immune-modulating vitro. Our goal was to determine if BC30 improved C. difficile-induced colitis mice. Starting on study day 0, female C57BL/6 mice were dosed by oro-gastric gavage for 15 days with vehicle (saline) or (2 × 109 CFU per day). Mice the groups received an...
Abstract Background Recently, we found that the probiotic strain Bacillus coagulans GBI-30, 6086 (GanedenBC 30 ) improved indices of Clostridium difficile (C. difficile) -induced colitis in mice (Fitzpatrick et al., Gut Pathogens , 2011). Our goal was to determine if BC30 could also prevent recurrence C. mice, following initial treatment with vancomycin. During study days 0 through 5, were treated antibiotics. On day 6, VPI 10463 given by oro-gastric gavage at ≈ 5x10 4 CFU induce colitis....
Eight calves were inoculated into the bronchus with H. somnus. Thirteen bovine respiratory syncytial virus (BRSV) and 8 days later All developed necrotizing, suppurative, lobular bronchopneumonia pleuritis. Clinical signs of disease pneumonic lesions significantly more severe in that sequentially BRSV followed by Pneumonic similar to those described for naturally occurring somnus-associated tract disease. Control alone or sham-inoculated medium did not develop clinical The BRSV-inoculated...
The AmpliVue HSV 1+2 assay was compared to the ELVIS ID and D(3) Typing Culture System for qualitative detection differentiation of herpes simplex virus 1 (HSV-1) HSV-2 DNA in 1,351 cutaneous mucocutaneous specimens. Compared ELVIS, had sensitivities 95.7 97.6% detecting HSV-1 HSV-2, respectively. Following arbitration discordant results by an independent molecular method, a resolved sensitivity specificity 99.2 99.7%, respectively, both whereas 87.1% 84.5% HSV-2.
Antimicrobial resistance, particularly in pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), limits treatment options and increases healthcare costs. To understand patient risk factors, including household animal contact, potentially associated with colonization multidrug-resistant MRSA isolates, we performed a prospective study of case patients colonized on admission to rural tertiary care hospital. Patients were interviewed antimicrobial resistance patterns tested among...
Background While methicillin-resistant Staphylococcus aureus (MRSA) originally was associated with healthcare, distinct strains later emerged in patients no prior hospital contact. The epidemiology of MRSA continues to evolve. Methods To characterize the current MRSA-colonized entering a serving both rural and urban communities, we interviewed MRSA-positive admission nasal swabs between August 2009 March 2010. We applied hospitalization risk factor, antimicrobial resistance phenotype,...
A 49-year-old male presented to the Emergency Department with 7 days of subjective fevers, headaches, muscle aches, increasing shortness-ofbreath, and nonproductive cough.One his family members had been tested positive for SARS-CoV-2 3 ago.A review patient's past medical history revealed he chronic lymphocytic leukemia (CLL) Rai stage IV, Binet C, complex cytogenetics, on daily venetoclax an obinutuzumab/venetoclax regimen.His was also notable uncontrolled insulin-dependent diabetes mellitus...
Abstract: Background: We have previously demonstrated that transplanting porcine encephalomyocarditis virus (EMCV)‐infected islet cells (PICs) results in transmission of the to recipient mice, which is manifested by acute fatal infection within 5 8 days. Here, we determined PIC susceptibility a related and highly prevalent human picornavirus, coxsackie B‐5 (CVB‐5). Methods: PICs were inoculated with CVB‐5 vitro for up 96 hours infectivity, level replication, cellular function determined....
Background: Here we compare the performance of high-throughput BD COR System (COR) to Viper LT (Viper) using Onclarity HPV assay.Research Design and Methods: Remnant clinical specimens, contrived specimens in SurePath (BD) PreservCyt (Hologic) media, prospective Cervical Brush Diluent (CBD) were tested. Outcomes included intra-laboratory agreement results on inter-system between Viper.Results: reproducibility resulted standard deviation correlation variation Ct values ranging from 0.14 1.98...
Journal Article Survival of Moraxella bovis in the Alimentary Tract Face Fly Get access H. W. Glass, Jr., Jr. University Tennessee. Knoxville. Tennessee 37901 Search for other works by this author on: Oxford Academic PubMed Google Scholar R. Gerhardt, Gerhardt Greene Economic Entomology, Volume 75, Issue 3, 1 June 1982, Pages 545–546, https://doi.org/10.1093/jee/75.3.545 Published: 01 1982 history Received: 11 May 1981
Detection of Bordetella pertussis and parapertussis using molecular methods is sensitive specific with a short turnaround time compared to other diagnostic methods. In this multicenter study, we the performance Simplexa Direct kit those assays in detecting differentiating B. nasopharyngeal swab specimens. The limits detection (LODs) were 150 CFU/ml or 3 fg/μl DNA for 1,500 10 A total 1,103 fresh residual frozen specimens from eight clinical sites tested. Combining data individual different...
This study determined the precision and reproducibility of results for BD CTGCTV2 (CTGCTV2) assay on COR System (COR). The clinical performance conducted was compared with its MAX (MAX) detecting Chlamydia trachomatis, Neisseria gonorrhoeae, or Trichomonas vaginalis. multiday multisite studies were using contrived panels positive negative urine PreservCyt specimens. A total 433 panel members, generated from remnant specimens, tested in comparison study. Each member three times COR. same...
We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop
Practice guidelines for infectious disease testing at the point of care (POC) have been developed by National Academy Clinical Biochemistry. These were established through extensive review scientific literature that addressed occurred POC. A total 25 established, and in general, it was concluded (a) rapid tests group streptococcal infections provided clinically useful financially justified results; (b) there insufficient evidence to recommend POCT B Streptococcus screening, however molecular...
Abstract Kidney transplant from donors with hepatitis C virus (HCV) antibody has been limited to HCV viremic recipients only, due concern of the transmission. However, new antiviral medications provide an opportunity expand utilization these donors. To study risk transmission in kidney transplantation, we used discarded donor kidneys and determined RNA levels by quantitative real‐time PCR bilateral (right left) biopsies plasma 14 antibody‐positive (sensitivity: 15 international unit (IU)/mL...