A5027276191- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Plant Virus Research Studies
- Microfluidic and Capillary Electrophoresis Applications
- Respiratory viral infections research
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- CCD and CMOS Imaging Sensors
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
Pennsylvania State University
2022-2025
Quantification of HIV RNA in plasma is critical for identifying the disease progression and monitoring effectiveness antiretroviral therapy. While RT-qPCR has been gold standard viral load quantification, digital assays could provide an alternative calibration-free absolute quantification method. Here, we reported a Self-digitization Through Automated Membrane-based Partitioning (STAMP) method to digitalize CRISPR-Cas13 assay (dCRISPR) amplification-free HIV-1 RNAs. The Cas13 was designed,...
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid-sensing systems have grown rapidly in the past few years. Nevertheless, an objective approach to benchmark performances of different CRISPR sensing is lacking due heterogeneous experimental setup. Here, we developed a quantitative figure merit (FOM) compare methods and explore performance improvement strategies. The FOM defined as product limit detection (LOD) associated reaction time (T). A smaller means...
Loop-mediated isothermal amplification (LAMP) is a rapid, sensitive, and cost-effective method for developing point-of-care nucleic acid testing due to its nature. Yet, LAMP can suffer from the issue of false positives, which compromise specificity results. positives typically arise contamination, nonspecific amplification, signal reporting (intercalating dyes, colorimetric, turbidity, etc.). While dye-labeled primers or probes have been introduced multiplexed detection enhanced in assays,...
Loop-mediated isothermal amplification (LAMP) is a promising method for point-of-care nucleic acid testing due to its simplicity, rapidity, and high sensitivity. Coupling LAMP with solid-state nanopores enables label-free, single-molecule sensing, enhancing diagnostic accuracy. However, conventional LAMP-coupled nanopore protocols require high-salt buffers (>1 M) improve signal strength translocation frequency, complicating workflows increasing contamination risks. In native (50 mM KCl),...
Abstract Respiratory viral infections pose a significant global public health challenge, partly due to the difficulty in rapidly and accurately distinguishing between viruses with similar symptoms at point of care, hindering timely appropriate treatment limiting effective infection control prevention efforts. Here, we developed multiplexed, non- invasive saliva-based, reverse transcription loop-mediated isothermal amplification (RT- LAMP) test that enables simultaneous detection three most...
Most children with SARS-CoV-2 have mild or asymptomatic symptoms, but some develop severe complications. Early identification of high-risk cases is crucial for timely intervention. Alterations in salivary microRNA (miRNA) levels serve as biomarkers severity prediction. However, a rapid, non-invasive method needed to quantify miRNA level changes an alternative sequencing. Here, we developed highly specific and sensitive ligation-recombinase polymerase amplification (RPA) assay quantifying...
DNA sequencing is a powerful tool for diagnosing conditions like infectious diseases and cancers. Even though current workflows demand rigorous quality control (QC) of samples, this QC typically limited to lab settings, despite recent advances in portable nanopore sequencers. For personalized healthcare truly benefit from the sequencer, must be performed right where samples are processed. Here, we present solid-state device that provides label-free, controlled quantification qualification...
The availability of effective antiretroviral therapy has made HIV manageable, provided patients have consistent access to routine viral load (VL) testing. Nonetheless, frequent VL testing remains limited. There is a need for accessible, user-friendly systems that allow people living with (PLHIV) monitor their more frequently and empower self-management. Here, we developed ViraLite, sample-to-answer, compact, battery-powered system monitoring. built upon probe-based RT-LAMP assay allows...
There is a significant demand for multiplexed fluorescence sensing and detection across range of applications. Yet, the development portable compact multiplexable systems remains substantial challenge. This difficulty largely stems from inherent need spectrum separation, which typically requires sophisticated expensive optical components. Here, we demonstrate compact, lens-free, cost-effective setup that incorporates machine learning scalable detection. method utilizes low-cost components...
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Human immunodeficiency virus (HIV) is a significant problem to consider as it can lead acquired immune deficiency syndrome (AIDS). Fortunately, AIDS manageable through antiretroviral therapy (ART). However, frequent viral load monitoring needed monitor the effectiveness of therapy. The current reverse transcription-polymerase chain reaction (RT-PCR) highly effective, but challenged by being resource-intensive and inaccessible, its turnaround time does not meet demand. An unmet need exists...
ProMagBot introduces scalable electromagnetic control of magnetic beads. The device is a handheld, battery-powered, and field-deployable sample preparation that can extract viral RNA from plasma samples in under 20 minutes.
To enable faster, easier extraction of viral RNA outside traditional laboratories, we use a custom, automated, and portable centrifuge for processing Qiagen QIAamp extractions. Viral is eluted into approx. 60 µl nuclease free water that can then be analyzed by downstream testing (PCR, gel electro, Qubit). Our results showed very strong agreement with benchtop extractions probit analysis demonstrated limiting concentration 0.75 cp/µl at 95% confidence.
Nucleic acid testing (NAT) has revolutionized diagnostics by providing precise, rapid, and scalable detection methods for diverse biological samples. These recent advancements satisfy the increasing demand on-site diagnostics, yet sample preparation remains a significant bottleneck achieving highly sensitive diagnostic assays. There is an unmet need compatible, efficient, lab-free point-of-care NAT. To address this, we developed portable, lab-free, battery-powered device extracting nucleic...
ABSTRACT The development of new nucleic acid techniques to quantify HIV RNA in plasma is critical for identifying the disease progression and monitoring effectiveness antiretroviral therapy. While RT-qPCR has been gold standard viral load quantification, digital assays could provide an alternative calibration-free absolute quantification method. Here, we report a s elf-digitalization t hrough utomated m embrane-based p artitioning (STAMP) technique digitalize CRISPR-Cas13 assay (dCRISPR)...
Here, we demonstrate a membrane-based digital CRISPR-Cas13a system for amplification-free absolute quantification of viral particles HIV-1. We established stamping technique to digitalize the Cas13 assay inside commercial track-etched polycarbonate (PCTE) membrane. evaluated performance our by quantifying synthetic HIV-1 RNA, where limit detection 100 aM was achieved in 30 min reaction. Absolute plasma background using confirmed that can quantify spiked samples.
For numerous diseases across the globe, nucleic acid testing remains clinical standard.However, there is a need for these methods to be field deployable.Recent improvements in optical detection and isothermal amplification have pushed POCT devices closer laboratory standards.However, sample preparation bottleneck that has not seen improvements.Here we present an automated device using paramagnetic beads extraction under 10 minutes.Linking our with microfluidics creates system fully...