Nucleic acid detection methods are crucial for many fields such as pathogen and genotyping. Solid-state nanopore sensors represent a promising platform nucleic due to its unique single molecule sensitivity label-free electronic sensing. Here, we demonstrated the use of glass highly sensitive quantification single-stranded circular DNAs (reporters), which could be degraded under trans-cleavage activity target-specific CRISPR-Cas12a. We developed optimized Cas12a assay HIV-1 analysis....

Label-free nanopore sensors have emerged as a new generation technology of DNA sequencing and been widely used for single molecule analysis. Since the first α-hemolysin biological nanopore, various types nanopores made different materials under extensive development. Noise represents common challenge among all sensors. The noise can be decomposed into four components in frequency domain (1/f noise, white dielectric amplifier noise). In this work, we reviewed summarized physical models,...

This article presents an innovative system for recovering thermal energy using a thermoelectric generator (TEG). The uses waste heat from water heater to generate electricity. A maximum temperature gradient of 96.5°C is maintained between the hot surface and sink, which cold-water cavity cooling TEG maintain good differential. produces about 350% more than consumes. electricity generated regulated by DC-DC converter stored in Li-ion battery, ensuring stable operation even after has been shut...

Solid-state nanopores have shown great promise and achieved tremendous success in label-free single-molecule analysis. However, there are three common challenges solid-state nanopore sensors, including the size variations from batch to that makes interpretation of sensing results difficult, incorporation sensor specificity, impractical analysis time at low analyte concentration due diffusion-limited mass transport. Here, we demonstrate a novel loop-mediated isothermal amplification...

Although promising, in many applications electrocoagulation (EC) is not adequate for decreasing pollutants to permissible levels discharge as per environmental standards. In this study, treatment of industrial wastewater from a textile dyeing plant was investigated by combined process EC, followed the use γ - F e 2 O 3 (Maghemite) nanoparticle adsorbents. The properties synthesized nanoparticles were determined XRD, SEM and FTIR analyses. effect aeration rate (0–2.65 L/min), concentration...

Unsteady settling behavior of solid spherical particles falling in water as a Newtonian fluid is investigated using drag coefficient the form given by Ferreira et al. Differential transformation method (DTM), Galerkin (GM), collocation (CM), and numerical methods are applied to analyze characteristics motion. The influence physical parameters on terminal velocity discussed moreover, comparing techniques, it showed that GM CM very efficient for solving governing equation DTM with Padé...

Nanopore sensor conceptually represents an ideal single molecule counting device due to its unique partitioning-free, label-free electronic sensing. Existing theories and experiments have shown that sample concentration is proportional the translocation rate. However, a detailed nanopore geometry size characterization or calibration curve of standards are often required for quantifying unknown sample. In this work, we proposed validated calibration-free digital method isolated...

Abstract Mechanical properties have emerged as a significant label-free marker for characterizing deformable particles such cells. Here, we demonstrated the first single-particle-resolved, cytometry-like deformability-activated sorting in continuous flow on microfluidic chip. Compared with existing deformability-based techniques, device presented this work measures deformability and immediately sorts one-by-one real time. It integrates transit-time-based measurement active hydrodynamic onto...

While the solid-state nanopore sensors have shown exceptional promise with their single-molecule sensitivity and label-free operations, one of most significant challenges in sensor is limited analyte translocation event rate that leads to prolonged response time. This issue more pronounced when concentration below nanomolar (nM) range, owing diffusion-limited mass transport. In this work, we systematically studied experimental factors beyond intrinsic electrophoretic mobility affect glass...

The high voltage power supply for magnetron, used the microwaves generators in industrial applications is a classical design. This system composed of single phase leakage flux transformer supplying cell capacitor and diode, which double stabilize current. An improved π quadruple model this developed taking into account saturation phenomena. It based on determination analytic inductances deduced by new approach fitting nonlinear curve B-H. Two parts are considered representation: linear...

Laser-assisted controlled dielectric breakdown (LaCBD) has emerged as an alternative to conventional CBD-based nanopore fabrication due its localization capability, facilitated by the photothermal-induced thinning down in hot spot. Here, we reported potential impact of laser on forming debris around region LaCBD. The was clearly observable scanning electron microscopy (SEM) and photoluminescence (PL) spectroscopy. We found that formation is a unique phenomenon LaCBD not CBD approach. also...

Abstract Controlled molecular transport and separation is of significant importance in various applications. In this work, we presented a novel concept nanofluidic charge-coupled device (CCD) for controlled DNA separation. By leveraging the unique field-effect coupling systems, CCD aims to store charged biomolecules such as DNAs discrete regions nanochannels transfer separate these charge packet bucket brigade fashion. We developed quantitative model capture impact nanochannel surface...

In this paper, Least Square Method (LSM) and Differential Transformation (DTM) are used to solve the problem of laminar nanofluid flow in a semi-porous channel presence transverse magnetic field. Due existence some shortcomings each method, novel efficient method named LS-DTM is introduced which omitted those defects has an excellent agreement with numerical solution. present study, effective thermal conductivity viscosity calculated by Maxwell–Garnetts (MG) Brinkman models, respectively....

This study focuses on the reduced-order modeling and operational optimization of heating network Université Libre de Bruxelles (ULB) to decrease its energy consumption CO2 emissions. A model was developed, alternatives current operation were investigated minimize cost. As a very first improvement, replacing fixed-speed pumps with optimally operated variable-speed pumps, pumping can be reduced by up 96 %. Further optimizing operating temperatures lead reduction energy, heat losses emissions...

Introduction: The purpose of this study was to investigate the demographic, clinical, and laboratory risk factors associated with mortality in Coronavirus disease 2019 (COVID-19) patients who were admitted intensive care unit (ICU) Iran. Material & Methods: This retrospective analyzed data from 867 COVID-19 ICU Shahid Beheshti Hospital Hamadan (west Iran) September 2020 March 2022. association between rates assessed using univariable multiple logistic regression analysis. Results: During...