A5100745080- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Chemical Synthesis and Analysis
- Plant biochemistry and biosynthesis
- Enzyme Production and Characterization
- Viral gastroenteritis research and epidemiology
- Liver physiology and pathology
- Porphyrin Metabolism and Disorders
- Metal-Catalyzed Oxygenation Mechanisms
- Mass Spectrometry Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Macrophage Migration Inhibitory Factor
- Metalloenzymes and iron-sulfur proteins
- Bacteriophages and microbial interactions
Wuhan University
2020-2024
City University
2024
Hangzhou City University
2024
Chinese Academy of Sciences
2009-2023
Anhui University
2021-2023
State Council of the People's Republic of China
2023
Hefei Institutes of Physical Science
2023
Taiyuan University of Technology
2010-2022
Sichuan University
2022
West China Second University Hospital of Sichuan University
2022
Preparedness for a possible influenza pandemic caused by highly pathogenic avian A subtype H5N1 has become global priority. The spread of the virus to Europe and continued human infection in Southeast Asia have heightened concern. It remains unknown from where strain may emerge; current attention is directed at Vietnam, Thailand, and, more recently, Indonesia China. Here, we report that genetically antigenically distinct sublineages established poultry different geographical regions Asia,...
Abstract NIR-II fluorophores have shown great promise for biomedical applications with superior in vivo optical properties. To date, few small-molecule been discovered donor-acceptor-donor (D-A-D) or symmetrical structures, and upconversion-mitochondria-targeted dyes not reported. Herein, we report development of D-A type thiopyrylium-based frequency upconversion luminescence (FUCL) at ~580 nm upon excitation ~850 nm. H4-PEG-PT can only quickly effectively image mitochondria live fixed...
H9N2 influenza viruses have become established in terrestrial poultry different Asian countries over the last 2 decades. Our previous study demonstrated that quail harbor increasingly diverse novel reassortants, including both Chicken/Beijing/1/94 (Ck/Bei-like) and Quail/Hong Kong/G1/97 (G1-like) viruses. However, since 1999, genesis evolution of types not been investigated systematically. In present study, isolated from chickens, ducks, other minor species were characterized genetically...
Thiopeptides, with potent activity against various drug-resistant pathogens, contain a characteristic macrocyclic core consisting of multiple thiazoles, dehydroamino acids, and 6-membered nitrogen heterocycle. Their biosynthetic pathways remain elusive, in spite great efforts by vivo feeding experiments. Here, cloning, sequencing, characterization the thiostrepton siomycin A gene clusters unveiled paradigm for thiopeptide specific formation, featuring ribosomally synthesized precursor...
Nosiheptide (NOS), belonging to the e series of thiopeptide antibiotics that exhibit potent activity against various bacterial pathogens, bears a unique indole side ring system and regiospecific hydroxyl groups on characteristic macrocyclic core. Here, cloning, sequencing, characterization nos gene cluster from Streptomyces actuosus ATCC 25421 as model for this thiopeptides has unveiled new insights into their biosynthesis. Bioinformatics-based sequence analysis in vivo investigation...
A ratiometric fluorescent probe based on a positively charged benzo[e]indolium moiety for bisulfite is reported. The underwent 1,4-addition reaction with the C-4 atom in ethylene group. This resulted large emission wavelength shift (Δλ = 106 nm) and an observable color change from orange to cyan. could be completed 90 s PBS buffer solution displayed high selectivity sensitivity bisulfite. simple paper test strip system was developed detect rapidly. Probe 1 used real samples good recovery.
Highly pathogenic avian influenza (HPAI) H5N1 viruses are now endemic in many Asian countries, resulting repeated outbreaks poultry and increased cases of human infection. The immediate precursor these HPAI is believed to be A/goose/Guangdong/1/96 (Gs/GD)-like first detected Guangdong, China, 1996. From 2000 onwards, novel reassortant or genotypes have emerged southern China. However, precursors the Gs/GD-like their subsequent reassortants not been fully determined. Here we characterize...
Abstract From January 2004 through June 2008, surveillance of dead wild birds in Hong Kong, People's Republic China, periodically detected highly pathogenic avian influenza (HPAI) viruses (H5N1) individual from different species. During this period, no subtype H5N1 were poultry on farms and markets Kong despite intensive surveillance. Thus, these findings demonstrate the potential for to disseminate HPAI areas otherwise free viruses. Genetic antigenic characterization 47 isolated showed that...
ABSTRACT An H6N1 virus, A/teal/Hong Kong/W312/97 (W312), was isolated during the “bird flu” incident in Hong Kong 1997. Genetic analysis suggested that this virus might be progenitor of A/Hong Kong/156/97 (HK/97) H5N1 as seven eight gene segments those viruses had a common source. Continuing surveillance showed W312-like prevalent quail and pheasants 1999; however, further development has not been investigated since 2001. Here we report influenza data collected southern China from 2000 to...
ABSTRACT The transmission of highly pathogenic avian influenza H5N1 virus to Southeast Asian countries triggered the first major outbreak and wave in late 2003, accelerating pandemic threat world. Due lack surveillance prior these outbreaks, genetic diversity pathways viruses from this period remain undefined. To determine possible source 1 viruses, we recently conducted further sequencing analysis samples collected live-poultry markets Guangdong, Hunan, Yunnan southern China 2001 2004....
ABSTRACT Two-way transmission of influenza viruses between humans and swine has been frequently observed, the occurrence 2009 H1N1 pandemic virus (pdm/09) demonstrated that swine-origin could facilitate genesis a strain. Although multiple introductions to reassortment in pdm/09 have repeatedly reported both Eurasia Americas, its long-term impact on development (SIVs) not systematically explored. Our comprehensive evolutionary studies complete genomes 387 SIVs obtained from 2012 by...
Domestic ducks in southern China act as an important reservoir for influenza viruses and have also facilitated the establishment of multiple H6 virus lineages. To understand continuing evolution these established lineages, 297 isolated from domestic during 2006 2007 were genetically antigenically analyzed. Phylogenetic analyses showed that group II duck had replaced previously predominant I lineage extended their geographic distribution coastal to inland regions. Group genesis development...
Terpenoid synthases catalyze isoprenoid cyclization reactions underlying the generation of more than 80,000 natural products. Such dramatic chemodiversity belies fact that these enzymes generally consist only three domain folds designated as α, β, and γ. Catalysis by class I terpenoid occurs exclusively in α domain, which is found with αα, αβ, αβγ architectures. Here, we explore influence architecture on catalysis taxadiene synthase from Taxus brevifolia (TbTS, αβγ), fusicoccadiene Phomopsis...
Abstract Bt DyP from Bacteroides thetaiotaomicron (strain VPI‐5482) and TyrA Shewanella oneidensis are dye‐decolorizing peroxidases (DyPs), members of a new family heme‐dependent recently identified in fungi bacteria. Here, we report the crystal structures BtDyP at 1.6 2.7 Å, respectively. assembles into hexamer, while dimer; dimerization interface is conserved between two proteins. Each monomer exhibits two‐domain, α+β ferredoxin‐like fold. A site for heme binding was computationally,...
TyrA is a member of the dye-decolorizing peroxidase (DyP) family, new family heme-dependent recently identified in fungi and bacteria. Here, we report crystal structure complex with iron protoporphyrin (IX) at 2.3 A. dimer, each monomer exhibiting two-domain, alpha/beta ferredoxin-like fold. Both domains contribute to heme-binding site. Co-crystallization presence an excess chloride allowed for unambiguous location active site specific residues involved heme binding. The reveals Fe-His-Asp...
Dipeptidyl-peptidase VI from Bacillus sphaericus and YkfC subtilis have both previously been characterized as highly specific γ-d-glutamyl-l-diamino acid endopeptidases. The crystal structure of a ortholog cereus (BcYkfC) at 1.8 Å resolution revealed that it contains two N-terminal bacterial SH3 (SH3b) domains in addition to the C-terminal catalytic NlpC/P60 domain is ubiquitous very large family cell-wall-related cysteine peptidases. A bound reaction product (l-Ala-γ-d-Glu) enabled...
The carboxyl-terminal amide group has been often found in many bioactive peptide natural products, including nosiheptide belonging to the over 80 entity-containing thiopeptide family. Upon functional characterization of a novel protein NosA biosynthesis, herein we report an unusual C-terminal forming strategy general for maturating certain amide-terminated thiopeptides by processing their precursor peptides featuring serine extension. acts on intermediate bearing bis-dehydroalanine tail and...
Thiopeptides are a growing class of sulfur-rich, highly modified heterocyclic peptides that mainly active against Gram-positive bacteria including various drug-resistant pathogens. Recent studies also reveal many thiopeptides inhibit the proliferation human cancer cells, further expanding their application potentials for clinical use. Thiopeptide biosynthesis shares common paradigm, featuring ribosomally synthesized precursor peptide and conserved posttranslational modifications, to afford...
To investigate the three-dimensional displacement of midshaft clavicular fractures after closed reduction and fixation with titanium elastic intramedullary nails. From January 2019 to 2023, 74 patients clavicle (classified according AO/OTA system as groups A, B, C) underwent nail fixation. Preoperative bilateral CT data postoperative affected were recorded processed using Mimics software. Clavicle length, shortening, separation, rotational along X/Y/Z axes measured recorded. Within-group...