A5046669322- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Microbial Metabolic Engineering and Bioproduction
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Viral Infections and Vectors
- Plant biochemistry and biosynthesis
- Vibrio bacteria research studies
- vaccines and immunoinformatics approaches
- Virology and Viral Diseases
Rice University
2024
University of Southern California
2021
Shanghai Institutes for Biological Sciences
2021
University of Hong Kong
2007-2008
Shantou University
2007-2008
HKU-Pasteur Research Pole
2007
Although many novel members of the Coronaviridae have recently been recognized in different species, ecology coronaviruses has not established. Our study indicates that bats harbor a much wider diversity than any other animal species. Dating coronavirus lineages suggests bat are older those animals and human severe acute respiratory syndrome (SARS) was directly derived from viruses wild wet markets southern China. Furthermore, most closely related SARS diverged 1986, an estimated divergence...
H9N2 influenza viruses have become established in terrestrial poultry different Asian countries over the last 2 decades. Our previous study demonstrated that quail harbor increasingly diverse novel reassortants, including both Chicken/Beijing/1/94 (Ck/Bei-like) and Quail/Hong Kong/G1/97 (G1-like) viruses. However, since 1999, genesis evolution of types not been investigated systematically. In present study, isolated from chickens, ducks, other minor species were characterized genetically...
Highly pathogenic avian influenza (HPAI) H5N1 viruses are now endemic in many Asian countries, resulting repeated outbreaks poultry and increased cases of human infection. The immediate precursor these HPAI is believed to be A/goose/Guangdong/1/96 (Gs/GD)-like first detected Guangdong, China, 1996. From 2000 onwards, novel reassortant or genotypes have emerged southern China. However, precursors the Gs/GD-like their subsequent reassortants not been fully determined. Here we characterize...
ABSTRACT An H6N1 virus, A/teal/Hong Kong/W312/97 (W312), was isolated during the “bird flu” incident in Hong Kong 1997. Genetic analysis suggested that this virus might be progenitor of A/Hong Kong/156/97 (HK/97) H5N1 as seven eight gene segments those viruses had a common source. Continuing surveillance showed W312-like prevalent quail and pheasants 1999; however, further development has not been investigated since 2001. Here we report influenza data collected southern China from 2000 to...
ABSTRACT The transmission of highly pathogenic avian influenza H5N1 virus to Southeast Asian countries triggered the first major outbreak and wave in late 2003, accelerating pandemic threat world. Due lack surveillance prior these outbreaks, genetic diversity pathways viruses from this period remain undefined. To determine possible source 1 viruses, we recently conducted further sequencing analysis samples collected live-poultry markets Guangdong, Hunan, Yunnan southern China 2001 2004....
Abstract β‐farnesene is a sesquiterpenoid with various industrial applications which now commercially produced by Saccharomyces cerevisiae strain obtained random mutagenesis and genetic engineering. We rationally designed genetically defined Yarrowia lipolytica through recovery of L‐leucine biosynthetic route, gene dosage optimization synthase disruption the competition pathway. The resulting titer was improved from 8 to 345 mg L ‐1 . Finally, strategy for decreasing lipid accumulation...
Abstract Cell engineering is commonly limited to the serial manipulation of a single gene or locus. The recently discovered CRISPR-associated transposases (CASTs) could manipulate multiple sets genes achieve predetermined cell diversity, with orthogonal CASTs being able them in parallel. Here, novel CAST from Pseudoalteromonas translucida KMM520 (PtrCAST) was characterized without protospacer adjacent motif (PAM) preference which can high insertion efficiency for larger cargo and multiplexed...
Directed evolution and targeted genome editing have been deployed to create genetic variants with usefully altered phenotypes. However, these methods are limited high-throughput screening or serial manipulation of single genes. In this study, we implemented multicopy chromosomal integration using CRISPR-associated transposases (MUCICAT) simultaneously target up 11 sites on the Escherichia coli chromosome for multiplex gene interruption and/or insertion, generating combinatorial genomic...
Vaccines are the main pharmaceutical intervention used against global public health threat posed by influenza viruses. Timely selection of optimal seed viruses with matched antigenicity between vaccine antigen and circulating high yield underscore efficacy supply, respectively. Current methods for selecting vaccines labor intensive time-consuming. Here, we report Machine-learning Assisted Influenza VaccinE Strain Selection framework, MAIVeSS, that enables streamlined naturally circulating,...
Abstract Migratory waterfowl, gulls, and shorebirds serve as natural reservoirs for influenza A viruses, with potential spillovers to domestic poultry humans. The intricacies of interspecies adaptation among avian species, particularly from wild birds poultry, are not fully elucidated. In this study, we investigated the molecular mechanisms underlying species barriers in H7 transmission, factors responsible disproportionate distribution infected A/Anhui/1/2013 (AH/13)-lineage H7N9 viruses....
The CRISPR-associated transposase system enables site-specific DNA integration on the genome independent of homologous recombination. Previous studies have demonstrated that type V-K Tn7-like from Scytonema hofmanni and I-F Vibrio cholerae strong target immunity like Tn7, therefore two or more copies donor would not be inserted into same location in theory. In this paper, we report can insert multiple one site, which was identified confirmed by single-strain identification high-throughput...
ABSTRACT Between 2013 and 2018, the novel A/Anhui/1/2013 (AH/13)-lineage H7N9 virus caused at least five waves of outbreaks in humans, totaling 1,567 confirmed human cases China. Surveillance data indicated a disproportionate distribution poultry infected with this AH/13-lineage virus, laboratory experiments demonstrated that can efficiently spread among chickens but not Pekin ducks. The underlying mechanism selective transmission remains unclear. In study, we absence Neu5Gc expression...