A5035480094- Barrier Structure and Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Kruppel-like factors research
- Pregnancy and preeclampsia studies
- Immune cells in cancer
- Mosquito-borne diseases and control
- Caveolin-1 and cellular processes
- Genomics and Chromatin Dynamics
- Erythrocyte Function and Pathophysiology
- dental development and anomalies
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Inflammasome and immune disorders
- RNA modifications and cancer
- interferon and immune responses
- Gut microbiota and health
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
Harvard University
2018-2025
Center for Systems Biology
2021-2025
Rutgers Sexual and Reproductive Health and Rights
2024
Rutgers, The State University of New Jersey
2024
Stanford University
2015
Directed DNA methylation on N6-adenine (6mA), N4-cytosine (4mC), and C5-cytosine (5mC) can potentially increase coding capacity regulate a variety of biological functions. These modifications are relatively abundant in bacteria, occurring about percent all bases most bacteria. Until recently, 5mC its oxidized derivatives were thought to be the only directed events metazoa. New more sensitive detection techniques (ultra-high performance liquid chromatography coupled with mass spectrometry...
Armor plate changes in sticklebacks are a classic example of repeated adaptive evolution. Previous studies identified ectodysplasin (EDA) gene as the major locus controlling recurrent loss freshwater fish, though causative DNA alterations were not known. Here we show that EDA alleles have cis-acting regulatory reduce expression developing plates and spines. An identical T → G base pair change is found enhancers divergent low-plated fish. Recreation marine enhancer strongly reduces posterior...
As an optically transparent model organism with endothelial blood-brain barrier (BBB), zebrafish offer a powerful tool to study the vertebrate BBB. However, precise developmental profile of functional BBB acquisition and subcellular molecular mechanisms governing remain poorly characterized. Here, we capture dynamics leakage using live imaging, revealing combination steady accumulation in parenchyma sporadic bursts tracer leakage. Electron microscopy studies further reveal high levels...
Abstract Flaviviruses pose a significant threat to public health due their ability infect the central nervous system (CNS) and cause severe neurologic disease. Astrocytes play crucial role in pathogenesis of flavivirus encephalitis through maintenance blood-brain barrier (BBB) integrity modulation immune cell recruitment activation within CNS. We have previously shown that receptor interacting protein kinase-3 (RIPK3) is coordinator neuroinflammation during CNS viral infection, function...
Glial-vascular interactions are critical for the formation and maintenance of brain blood vessels blood-brain barrier (BBB) in mammals, but their role zebrafish is not well understood. Our previous work has detailed timeline BBB functional maturation zebrafish, revealing a conserved mechanism induction through suppression endothelial transcytosis. Yet, as opposed to extensive research on glial-vascular rodents, such remain largely overlooked model system. Here, we focus development brain,...
ABSTRACT The blood-brain barrier (BBB) is a unique set of properties the brain vasculature which severely restricts its permeability to proteins and small molecules. Classic chick-quail chimera studies showed that these are not intrinsic but rather induced by surrounding neural tissue. Here we identify Spock1 as candidate neuronal signal for regulating BBB in zebrafish mice. Mosaic genetic analysis shows neuronally-expressed cell non-autonomously required functional BBB. Leakage spock1...
Abstract Brain endothelial cells (BECs) play an important role in maintaining central nervous system (CNS) homeostasis through blood-brain barrier (BBB) functions. BECs express low baseline levels of adhesion receptors, which limits entry leukocytes. However, the molecular mediators governing this phenotype remain mostly unclear. Here, we explored how infiltration immune across BBB is influenced by scaffold protein IQ motif containing GTPase activating 2 (IQGAP2). In mice and zebrafish,...
Abstract As an optically transparent model organism with endothelial blood-brain barrier (BBB), zebrafish offer a powerful tool to study the vertebrate BBB. However, precise developmental profile of functional BBB acquisition and subcellular molecular mechanisms governing remain poorly characterized. Here we find spatiotemporal gradient acquisition. Moreover, capture dynamics leakage using live imaging, revealing combination steady accumulation in parenchyma sporadic bursts tracer leakage....