A5066085553- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2015-2022
This study investigated the influence of nature oligonucleotides on abilities to form antiparallel and parallel duplexes. Base pairing homopurine DNA, 2'-O-MeRNA RNA with respective homopyrimidine as well chimeric containing LNA resulted in formation 18 various UV melting, circular dichroism fluorescence studies revealed nucleotide composition duplex structure thermal stability depending buffer pH value. Most duplexes simultaneously adopted both orientations. However, at 5.0, were more...
Abstract Thionucleotides, especially 4-thiouridine and 6-thioguanosine, are photosensitive molecules that photocrosslink to both proteins nucleic acids, this feature is a major reason for their application in various investigations. To get insight into the thermodynamic structural contributions of 6-thioguanosine properties RNA duplexes systematic study was performed. In series duplexes, selected guanosine residues located G-C base pairs, mismatches (G-G, G-U, G-A), or 5′ 3′-dangling ends...
Thrombin binding aptamer (TBA), is a short DNA 15-mer that forms G-quadruplex structure and possesses anticoagulant properties. Some chemical modifications, including unlocked nucleic acids (UNA), 2′-deoxy-isoguanosine 2′-deoxy-4-thiouridine were previously found to enhance the biological activity of TBA. In this paper, we present thermodynamic characteristics TBA variants have been modified with novel isoguanine derivative UNA as well isoguanosine. Additionally, UNA-4-thiouracil...
The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants ribozymes targeting conserved structural motifs segment 5 influenza A virus (IAV) (+)RNA. carry chemical modifications aiming to improve the cleavage activity introduced an additional hairpin motif attempted select ribozyme-target pairs with sequence features that enable potential formation trans-Hoogsteen interactions present in full-length, highly...