A5085241168- interferon and immune responses
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
Max Planck Institute for Dynamics of Complex Technical Systems
2024
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2019-2022
The influenza A virus is a human pathogen that poses serious public health threat due to rapid antigen changes and emergence of new, highly pathogenic strains with the potential become easily transmitted in population. viral genome encoded by eight RNA segments, all stages replication cycle are dependent on RNA. In this study, we designed small interfering (siRNA) targeting segment 5 nucleoprotein (NP) mRNA structural motifs encode important functions. new criterion for choosing siRNA target...
RNA structure in the influenza A virus (IAV) has been focus of several studies that have shown connections between conserved secondary motifs and their biological function replication cycle. Questions arisen on how to best recognize understand pandemic properties IAV strains from an perspective, but determination challenging. Herein, we used chemical mapping determine segment 8 viral (vRNA) A/California/04/2009 (H1N1) strain IAV. Additionally, this long, naturally occurring served as a model...
Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and "OP7", new type DIP discovered recently. Based on experimental data from
The functionality of RNA is fully dependent on its structure. For the influenza A virus (IAV), there are confirmed structural motifs mediating processes which important for viral replication cycle, including genome assembly and packaging. Although strains originating from distant IAV subtypes might fold differently, some conserved, thus, functionally important. Nowadays, NGS-based structure modeling a source new in vivo data helping to understand biology. However, accurate structures, these...
The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants ribozymes targeting conserved structural motifs segment 5 influenza A virus (IAV) (+)RNA. carry chemical modifications aiming to improve the cleavage activity introduced an additional hairpin motif attempted select ribozyme-target pairs with sequence features that enable potential formation trans-Hoogsteen interactions present in full-length, highly...