A5014926983- Influenza Virus Research Studies
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- Cancer Cells and Metastasis
- Wound Healing and Treatments
- Gene Regulatory Network Analysis
- Electrospun Nanofibers in Biomedical Applications
- Protein purification and stability
- Stochastic processes and statistical mechanics
- Nanofabrication and Lithography Techniques
- Protein Structure and Dynamics
- Animal Virus Infections Studies
- Collagen: Extraction and Characterization
- Diffusion and Search Dynamics
- Mathematical Biology Tumor Growth
Ludwig-Maximilians-Universität München
2017-2024
Max Planck Institute for Dynamics of Complex Technical Systems
2016-2024
Ibiden (Japan)
2023
Max Planck Society
2016-2021
Cancer cell migration is influenced by cellular phenotype and behavior as well the mechanical chemical properties of environment. Furthermore, many cancer cells show plasticity their adapt it to Here, we study influence fiber stiffness, confinement, adhesion on in porous collagen gels. Collagen gels with soft fibers abrogate promote a round, non-invasive phenotype. Stiffer are inherently more adhesive lead existence an general confined due adhesion. Addition TGF-β lowers adhesion, eliminates...
Defective interfering particles (DIPs) of influenza A virus (IAV) are naturally occurring mutants that have an internal deletion in one their eight viral RNA (vRNA) segments, rendering them propagation-incompetent. Upon coinfection with infectious standard (STV), DIPs interfere STV replication through competitive inhibition. Thus, proposed as potent antivirals for treatment the disease. To select corresponding candidates, we studied
Influenza A viruses (IAV) are commonly used to infect animal cell cultures for research purposes and vaccine production. Their replication is influenced strongly by the multiplicity of infection (MOI), which ranges over several orders magnitude depending on respective application. So far, mathematical models IAV have paid little attention impact MOI dynamics virus yields. To address this issue, we extended an existing model in adherent MDCK cells with kinetics that explicitly consider time...
Cell culture-derived defective interfering particles (DIPs) are considered for antiviral therapy due to their ability inhibit influenza A virus (IAV) production. DIPs contain a large internal deletion in one of eight viral RNAs (vRNAs) rendering them replication-incompetent. However, they can propagate alongside homologous standard (STV) during infection competition cellular and resources. So far, experimental modeling studies IAV have focused on either the intracellular or cell population...
Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and "OP7", new type DIP discovered recently. Based on experimental data from
Abstract Opposed to tubulin-binding agents, actin-binding small molecules have not yet become part of clinical tumor treatment, most likely due the fear general cytotoxicity. Addressing this problem, we investigated long-term efficacy sub-toxic doses miuraenamide, an actin filament stabilizing natural compound, on cell (SKOV3) migration. No cytotoxic effects or persistent morphological changes occurred at a concentration miuraenamide 20 nM. After 72 h treatment with concentration, nuclear...
Abstract A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures fibrils upon a pH shift in the presence of different concentrations Zn 2+ vitro . Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR) revealed existence higher order assembly : [6-D-Phe]. Nuclear Magnetic Resonance (NMR) indicated weak interaction between Atomic Force Microscopy (AFM) showed [6-D-Phe] oligomers fibrils. Molecular Dynamic...
The prion-like spreading of Tau pathology is the leading cause disease progression in various tauopathies. A critical step propagating pathologic brain transport from extracellular environment and accumulation inside naïve neurons. Current research indicates that human neurons internalize both physiological (eTau) monomers pathological eTau aggregates. However, similarities or differences neuronal mechanisms between species remain elusive.
<h3>Background</h3> T cell cytotoxicity plays a crucial role in defending against cancer. It is traditionally assessed by endpoint assays with non-microscopic appliance, thus lacking temporal and visual insights into middle time ranges that live-cell imaging offers. However, conventional falls short data scale due to limited replicates per condition. Combining micro-structured slides addresses these issues. Multiple adhesion patterns enable independent progression of killing processes,...
Abstract Defective interfering particles (DIPs) of influenza A virus (IAV) are naturally occurring mutants that comprise an internal deletion in one their eight viral RNA (vRNA) segments, rendering them propagation-incompetent. Upon co-infection with infectious standard (STV), DIPs interfere STV replication through competitive inhibition. Thus, proposed as potent antivirals for treatment the disease. To select corresponding candidates, we studied de novo generation and propagation...
The multiplicity of infection (MOI) is an important parameter for influenza A virus replication in animal cell culture. Depending on the respective question addressed research or vaccine production, a range comprising several orders magnitude typically covered. Mathematical models, however, have paid little attention to impact MOI dynamics so far. To overcome this limitation, we implemented kinetics that consider time point existing model replication. We find extended closely captures...
Abstract Endothelial tube formation on a reconstituted extracellular matrix (Matrigel) is well-established in vitro model for studying the processes of angiogenesis and vasculogenesis. However, to date, organizing principles that underlie morphogenesis this network, shape initial process cell-cell finding remain elusive. Furthermore, it unclear how results extrapolate vivo morphogenesis. Here, we identify mechanism allows cells form networks by mechanically reorganizing stiffening their...