A5078474229- Glaucoma and retinal disorders
- Corneal surgery and disorders
- Collagen: Extraction and Characterization
- Ophthalmology and Eye Disorders
- Cardiovascular Function and Risk Factors
- Elasticity and Material Modeling
- Protease and Inhibitor Mechanisms
- Cardiac Fibrosis and Remodeling
- Intraocular Surgery and Lenses
- Probabilistic and Robust Engineering Design
- Retinal Diseases and Treatments
- Structural Health Monitoring Techniques
- Cellular Mechanics and Interactions
- Ocular Surface and Contact Lens
- Fatigue and fracture mechanics
- Signaling Pathways in Disease
- Cerebral Venous Sinus Thrombosis
- Cardiomyopathy and Myosin Studies
- Ophthalmology and Visual Impairment Studies
- Bone Tissue Engineering Materials
- GDF15 and Related Biomarkers
- Corneal Surgery and Treatments
- Connective tissue disorders research
- Morphological variations and asymmetry
- Tendon Structure and Treatment
Johnson & Johnson (United States)
2020-2024
University of Pittsburgh
2016-2022
Visual Sciences (United States)
2016
The University of Texas at San Antonio
2009-2015
Jackson Memorial Hospital
2015
University of Mississippi Medical Center
2015
The University of Texas Health Science Center at San Antonio
2012-2015
University of Washington
2012
Harvey Mudd College
2007
Rationale: Matrix metalloproteinase (MMP)-28 regulates the inflammatory and extracellular matrix responses in cardiac aging, but roles of MMP-28 after myocardial infarction (MI) have not been explored. Objective: To determine impact deletion on post-MI remodeling left ventricle (LV). Methods Results: Adult C57BL/6J wild-type (n=76) MMP null (MMP-28 −/− , n=86) mice both sexes were subjected to permanent coronary artery ligation create MI. expression decreased post-MI, its cell source shifted...
In this study, we examined the combined effect of aging and myocardial infarction on left ventricular remodeling, focusing matrix metalloproteinase (MMP)-9-dependent mechanisms. We enrolled 55 C57BL/6J wild type (WT) 85 MMP-9 Null (Null) mice both sexes at 11–36 months age evaluated their response Day 7 post–myocardial infarction. Plasma levels positively linked to in WT ( r = .46, p .001). deletion improved survival (76% for vs 88% Null, .021). Post–myocardial infarction, there was a...
To model the sensitivity of optic nerve head (ONH) biomechanical environment to acute variations in IOP, cerebrospinal fluid pressure (CSFP), and central retinal artery blood (BP).We extended a previously published numerical ONH include 24 factors representing tissue anatomy mechanical properties, all three pressures, constraints on (CON). A total 8340 models were studied predict factor influences 98 responses two-step process: fractional factorial screening analysis identify 16 most...
Aging is linked to increased matrix metalloproteinase-9 (MMP-9) expression and extracellular turnover, as well a decline in function of the left ventricle (LV). Previously, we demonstrated that C57BL/6J wild-type (WT) mice > 18 mo age show impaired diastolic function, which was attenuated by MMP-9 deletion. To evaluate mechanisms initiate development cardiac dysfunction, compared LVs 6-9- 15-18-mo-old WT null (Null) mice. All groups showed similar LV echocardiography, indicating dysfunction...
Collagen fibers play a central role in normal eye mechanics and pathology. In ocular tissues, collagen exhibit complex 3-dimensional (3D) fiber orientation, with both in-plane (IP) out-of-plane (OP) orientations. Imaging techniques traditionally applied to the study of tissues only quantify IP providing little information on OP orientation. Accurate description 3D microstructures requires quantifying full Herein, we present 3dPLM, technique based polarized light microscopy developed...
Although collagen microstructural crimp is a major determinant of ocular biomechanics, no direct measurements optic nerve head (ONH) have been reported. Our goal was to characterize the period lamina cribrosa (LC) and peripapillary sclera (PPS) at low normal IOPs.ONHs from 11 sheep eyes were fixed 10-, 5-, or 0-mm Hg IOP periods measured manually coronal cryosections imaged with polarized light microscopy (PLM). Using linear mixed-effect models, we characterized LC PPS periods, how they...
Advances in imaging have made it increasingly common to study soft tissues without first embedding them plastic or paraffin and using labels stains. The process, however, usually still involves fixation cryosectioning, which could deform the tissues. Our goal was quantify morphological changes of ocular caused by formalin cryosectioning. From each 6 porcine eyes, 4 regions were obtained: cornea, equatorial posterior sclera, pole containing optic nerve head. Samples imaged visible light...
Purpose: The purpose of this study was to determine how the architecture lamina cribrosa (LC) microstructure, including shape and size pores, influences IOP-induced deformation neural tissues within LC pores using computational modeling. Methods: We built seven specimen-specific finite element models microstructure with distinct nonlinear anisotropic properties for beams based on histological sections from three sheep eyes. Changes in (aspect ratio convexity) (area perimeter length) due hoop...
The naked mole-rat (NMR) is a strictly subterranean rodent with low resting metabolic rate. Nevertheless, it can greatly increase its activity to meet the high energetic demands associated digging through compacted soils in xeric natural habitat where food patchily distributed. We hypothesized that NMR heart would naturally have basal function and exhibit large cardiac reserve, thereby mirroring species' metabolism scope. Echocardiography showed young (2–4 yr old) healthy NMRs fractional...
Adverse remodeling of the left ventricle (LV) following myocardial infarction (MI) leads to heart failure. Recent studies have shown that scar anisotropy is a determinant cardiac function post-MI, however it remains unclear how changes in extracellular matrix (ECM) organization and structure contribute LV function. The objective this study develop model identify potential mechanisms by which collagen affect post-MI.A four-region, multi-scale, cylindrical post-MI was developed. mechanical...
Purpose: To evaluate visual outcomes following the Smooth Incision Lenticular Keratomileusis (SILK TM ) procedure for correction of myopic refractive errors with and without astigmatism, using ELITA Femtosecond Laser System. Patients Methods: A prospective, multicenter, single-arm, open-label clinical study was conducted. Eighty-five subjects (n = 170 eyes), aged 18 years or older, manifest spherical equivalent (MRSE) up to − 12.00 D astigmatism 6.00 D, were treated binocularly femtosecond...
Purpose: The prevailing theory about the function of lamina cribrosa (LC) connective tissues is that they provide structural support to adjacent neural tissues. Missing would compromise this and therefore are regarded as "LC defects", despite scarce actual evidence their role. We examined how so-called LC defects alter IOP-related mechanical insult Methods: built numerical models incorporating microstructure from polarized light microscopy images. To simulate varying sizes, individual beams...
The purpose of this study was to visualize the lamina cribrosa (LC) capillaries and collagenous beams, measure capillary tortuosity (path length over straight end-to-end length), determine if changes when intraocular pressure (IOP) increases.Within 8 hours sacrifice, 3 pig heads were cannulated via external ophthalmic artery, perfused with PBS remove blood, then a fluorescent dye label capillaries. posterior pole each eye mounted in custom-made inflation chamber for control IOP simultaneous...
Cardiac aging is characterized by diastolic dysfunction of the left ventricle (LV), which due in part to increased LV wall stiffness. In phase, myocytes are relaxed and extracellular matrix (ECM) a critical determinant changes To evaluate effects ECM composition on cardiac aging, we developed mathematical model predict dimension stiffness mice integrating mechanical laws our experimental results. We measured dimension, thickness, mass, collagen content for wild type (WT) C57/BL6J ages...
Elevated intraocular pressure (IOP) deforms the lamina cribrosa (LC), a structure within optic nerve head (ONH) in back of eye. Evidence suggests that these deformations trigger events eventually cause irreversible blindness, and have therefore been studied in-vivo using optical coherence tomography (OCT), ex-vivo OCT diversity techniques. To best our knowledge, there no in-situ studies LC mechanics. Our goal was two-fold: to introduce technique for measuring 3D from OCT, determine whether...