A5061466081- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Advanced Biosensing Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Ion-surface interactions and analysis
- Analytical Chemistry and Chromatography
- Nicotinic Acetylcholine Receptors Study
- Cholinesterase and Neurodegenerative Diseases
- Cardiomyopathy and Myosin Studies
- Glycosylation and Glycoproteins Research
- Hemoglobin structure and function
- Receptor Mechanisms and Signaling
- Chemical synthesis and alkaloids
- Biochemical and Structural Characterization
- Environmental Education and Sustainability
- Chemical Synthesis and Analysis
- Protein purification and stability
- Enzyme Structure and Function
Karolinska Institutet
2021-2025
Uppsala University
2020-2023
Disordered regions are an important functional feature of many multidomain proteins. A prime example is proteins in membraneless organelles, which contain folded domains that engage specific interactions and disordered low-complexity (LC) mediate liquid-liquid phase separation. Studying these complex architectures remains challenging due to their conformational variability. Native mass spectrometry (nMS) routinely employed analyze conformations or proteins; however, its ability with LC has...
Integral membrane proteins carry out essential functions in the cell, and their activities are often modulated by specific protein-lipid interactions membrane. Here, we elucidate intricate role of cardiolipin (CDL), a regulatory lipid, as stabilizer complexes. Using silico -designed model protein TMHC4_R (ROCKET) scaffold, employ combination molecular dynamics simulations native mass spectrometry to explore features that facilitate preferential lipid mediate stabilization. We find spatial...
Integral membrane proteins carry out essential functions in the cell, and their activities are often modulated by specific protein-lipid interactions membrane. Here, we elucidate intricate role of cardiolipin (CDL), a regulatory lipid, as stabilizer complexes. Using silico -designed model protein TMHC4_R (ROCKET) scaffold, employ combination molecular dynamics simulations native mass spectrometry to explore features that facilitate preferential lipid mediate stabilization. We find spatial...
<p dir="ltr">Understanding biomolecular interactions is crucial for advancing our fundamental knowledge of biological processes and aiding pharmaceutical development to target diseases effectively. This thesis aims investigate how protein properties influence the charging mechanisms during electrospray ionization (ESI) in native mass spectrometry (nMS). We then use insigths from these studies structure-function relationship membrane protein-lipid complexes.</p><p...
<p dir="ltr">Understanding biomolecular interactions is crucial for advancing our fundamental knowledge of biological processes and aiding pharmaceutical development to target diseases effectively. This thesis aims investigate how protein properties influence the charging mechanisms during electrospray ionization (ESI) in native mass spectrometry (nMS). We then use insigths from these studies structure-function relationship membrane protein-lipid complexes.</p><p...
Integral membrane proteins carry out essential functions in the cell, and their activities are often modulated by specific protein-lipid interactions membrane. Here, we elucidate intricate role of cardiolipin (CDL), a regulatory lipid, as stabilizer complexes. Using silico -designed model protein TMHC4_R (ROCKET) scaffold, employ combination molecular dynamics simulations native mass spectrometry to explore features that facilitate preferential lipid mediate stabilization. We find spatial...
Surface plasmon resonance (SPR) biosensor methods are ideally suited for fragment-based lead discovery. However, generally applicable experimental procedures and detailed protocols lacking, especially structurally or physico-chemically challenging targets when tool compounds not available. Success depends on accounting the features of both target chemical library, purposely designing screening experiments identification validation hits with desired specificity mode-of-action, availability...
In solution, the charge of a protein is intricately linked to its stability, but electrospray ionization distorts this connection, potentially limiting ability native mass spectrometry inform about structure and dynamics. How behavior intact proteins in gas phase depends on presence distribution ionizable surface residues has been difficult answer because multiple chargeable sites are present virtually all proteins. Turning engineering, we show that side chains completely dispensable for...
Electrospray ionization mass spectrometry is increasingly applied to study the structures and interactions of membrane protein complexes. However, charging mechanism complicated by presence detergent micelles during ionization. Here, we show that final charge proteins can be predicted their molecular weight when released from non-charge reducing saccharide detergents. Our data indicate PEG detergents lower depending on number molecules in surrounding micelle, whereas fos-choline may...
Native mass spectrometry (nMS) provides insights into the structures and dynamics of biomacromolecules in their native-like states by preserving noncovalent interactions through "soft" electrospray ionization (ESI). For native proteins, number charges that are acquired scales with surface area mass. Here, we explore effect highly negatively charged DNA on ESI charge protein complexes find a reduction mass-to-charge ratio as well greater variation. The state distributions pure assemblies show...
Fragments inducing conformational changes identified at a dynamic region of AChBP.
Abstract Integral membrane proteins carry out essential functions in the cell, and their activities are often modulated by specific protein-lipid interactions membrane. Here, we elucidate intricate role of cardiolipin (CDL), a regulatory lipid, as stabilizer complexes. Using silico -designed model protein TMHC4_R (ROCKET) scaffold, employ combination molecular dynamics simulations native mass spectrometry to explore features that facilitate preferential lipid mediate stabilization. We find...
Abstract Surface plasmon resonance biosensor technology (SPR) is ideally suited for fragment-based lead discovery. However, generally suitable experimental procedures or detailed protocols are lacking, especially structurally physico-chemically challenging targets when tool compounds lacking. Success depends on accounting the features of both target and chemical library, purposely designing screening experiments identification validation hits with desired specificity mode-of-action,...