The cell-penetrating peptide R9, an oligoarginine comprising nine arginines, has been used to transport biological cargos into cells. However, the mechanisms underlying its translocation across membranes remain unclear. In this report, we investigated entry of carboxyfluorescein (CF)-labeled R9 (CF-R9) single giant unilamellar vesicles (GUVs) various lipid compositions and CF-R9-induced leakage a fluorescent probe, Alexa Fluor 647 hydrazide (AF647), using method developed recently by us....

The translocation of cell-penetrating peptides (CPPs) through plasma membranes living cells is an important physiological phenomenon in biomembranes. To reveal the mechanism underlying a CPP, transportan 10 (TP10), lipid bilayers, we examined effects mechanical properties bilayers on entry carboxyfluorescein (CF)-labeled TP10 (CF-TP10) into giant unilamellar vesicle (GUV) using single GUV method. First, effect lateral tension CF-TP10 GUVs comprising mixture dioleoylphosphatidylglycerol...

Malnutrition among the rural Bangladeshi women of reproductive age is still very high. This high prevalence attributes to a range adverse health consequences on and their offspring. A total 2341 aged between 20 45 years residing in study area were interviewed this cross-sectional study. Information socioeconomic variables, nutritional status, pregnancy-related history was obtained using interviewer administered questionnaire. 34% suffer from malnutrition. multivariate analysis shows...

Lactoferricin B (LfcinB) and shorter versions of this peptide have antimicrobial activity. However, the elementary processes interactions these peptides with lipid membranes bacteria are still not well understood. To elucidate mechanism their activity, we investigated LfcinB (4-9) (its sequence RRWQWR) Escherichia coli cells giant unilamellar vesicles (GUVs). lissamine rhodamine red-labeled (Rh-LfcinB (4-9)) did induce an influx a membrane-impermeant fluorescent probe, SYTOX green, from...

Abstract Objective: To assess knowledge and attitudes associated with the menopause transition among women in Bangladesh. Methods: A cross-sectional survey was conducted (age range 45-60 y), 160 participants were selected from both urban rural settings using a systematic sampling procedure. We used face-to-face interview techniques employing semistructured questionnaire. Bivariate multivariate regression analyses done to factors. Results: Around one-fourth (23%) of did not have basic...

Enzymes are biological molecules that act as catalysts and speed up the biochemical reactions. The world’s biotechnological ventures development of enzyme productiveness, advancement novel techniques for thriving their shelf existence. Nowadays, most burning questions in technology how to improve productivity reuse them. immobilization enzymes provides an excellent scope several times increase productivity. main aim present study is establishment immobilized multi-enzyme bio-system...

Viral diseases are one of the most life-threatening as they can erupt unpredictably and spread rapidly in any medium with a very small number particles. Therefore, key for lethal virus detection should be highly sensitive early-stage detection, which help increase chance survival. Amplification detecting signal is promising mechanisms low-concentration analytes. A proper amplification develop such kind system where particles produce intense signals prominent detection. Keeping this mind,...

Hospital-acquired infections endanger millions of lives around the world, and nurses play a vital role in prevention these infections. Knowledge infection control (IPC) best practices among is prerequisite to maintaining standard precautions for safety patients.The study aims assess knowledge, attitudes, (KAP) towards IPC including associated factors tertiary care hospital Bangladesh.We conducted this hospital-based cross-sectional from October 2017 June 2018 at Dhaka Medical College...

Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which series of high-voltage short pulses are used. As new approach, we aimed to investigate the rupture giant unilamellar vesicles (GUVs) using IRE under different osmotic pressures (Π), and estimated membrane tension due Π. Two categories GUVs were used this study. One was prepared with mixture dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) cholesterol (chol) for obtaining more...

The objective of the study was to evaluate pharmacological properties methanolic extract

The development of Optical Character Recognition (OCR) systems for Bangla script has been an area active research since the 1980s. This study presents a comprehensive analysis and cross-platform mobile application OCR, leveraging Tesseract OCR engine. primary objective is to enhance recognition accuracy characters, achieving rates between 90% 99%. designed facilitate automatic extraction text from images selected device's photo library, promoting preservation accessibility language...

Adolescents, aged 10-19 years, constitute one fifth of Bangladesh's total population 158 million. Similar to adolescents elsewhere, Bangladeshi also have special sexual and reproductive health (SRH) information service needs; however, these needs are not adequately addressed by the existing system. The present study was conducted identify adolescents’ expectations preferences for receiving SRH services. This qualitative in six purposively selected areas Bangladesh using 20 Key Informant...

Cell-penetrating peptides (CPPs) can enter the cytosol of eukaryotic cells without killing them whereas some CPPs exhibit antimicrobial activity against bacterial cells. Here, to elucidate mode interaction CPP nona-arginine (R

Distribution of F-Latency (DFL) is a new neurological parameter conceived by the Biomedical Physics group Dhaka University. Previous experimental studies have indicated that DFL, obtained from median nerve, varies with postures subject, particularly bending head. It agreed contention head might produce compression or stretching nerve fibers as they come out vertebra, and DFL sensitive gets affected even such temporary changes. The present work was taken up two objectives, i) to improve upon...

Aspergillus is a fungus found in the environment. In an immunocompetent person, inhalation of spores may cause localized infection. Invasive aspergillosis has poor prognosis. We describe case cerebral immune competent patient. A 30-year-old man was admitted with seizures and headaches. Magnetic resonance imaging (MRI) brain showed contrast enhancing lesion at suprasellar region. Excision biopsies granulomatous reactions, mixed inflammatory infiltration, fibrosis, necro-purulent material...

Introduction: The emergence of antibiotic-resistant organisms is a major public health concern, particularly in hospitals and other care settings. Carbapenem are group β- lactam antimicrobial agents with an exceptionally broad spectrum activity. They used as last resort drug against many multi-drug resistant (MDR) microorganisms such extended-spectrum beta-lactamase (ESBL), Metallo-β- lactamase (MBL), AmpC β enzyme-producing gram-negative bacilli. Aim the study: aim this study was to...

Background: Metallo-β-lactamases (MBL) have a wide spectrum of activity and they confer higher level resistance to all β-lactams antibiotics including Carbapenem. The active site in MBLs contains either 1 or 2 Zn2+ ions for their catalytic mechanism. All share common feature being inhibited by EDTA. Metallo-β-lactamase-producing gram-negative bacteria are the most important nosocomial pathogens. present study was conducted detect Metallo-β-lactamase production bacilli Combined Disc Synergy...

Ground water is the main and most important source of supply in Bangladesh. But has been contaminated by arsenic become a public health hazards our country. About 35-70 million people 61 out 64 districts Bangladesh are consuming prone to develop due chronic arsenicosis. Chronic exposure may cause wide range carcinogenic & non-carcinogenic effects. So far there no specific management for toxicity, though magnitude disease potentially toxic fatal. If effective treatments not provided those...

The population growth and urbanization of Rajshahi metropolitan city shows an increasing trend. This paper attempts to figure out the influence land use change on price in city.As resources are limited, resource is also limited fulfill demand for industrial, commercial, residential, institutional uses land. Land varies with different types uses. responsible price. To find effects price, a field survey was conducted within five selected wards area. Primary data gathered from required...