A5059922416- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Cardiac Fibrosis and Remodeling
- Cardiovascular Effects of Exercise
- Global Public Health Policies and Epidemiology
- Health disparities and outcomes
- Receptor Mechanisms and Signaling
- Cardiovascular Function and Risk Factors
- Global Health Care Issues
- Urinary Bladder and Prostate Research
- Cardiac Ischemia and Reperfusion
- Urologic and reproductive health conditions
- Advanced Fluorescence Microscopy Techniques
- Congenital heart defects research
- Streptococcal Infections and Treatments
- Pluripotent Stem Cells Research
- Bacteriophages and microbial interactions
- Autophagy in Disease and Therapy
- Salmonella and Campylobacter epidemiology
- RNA Research and Splicing
University of Utah
2012-2024
Rollins College
2024
Harrison Medical Center
2012-2019
Moscow Academy of Education Natalia Nesterova
2019
Johns Hopkins Medicine
2012
Johns Hopkins University
2012
University of California, Davis
2012
University of Buenos Aires
2005
Rationale: Cardiac resynchronization therapy (CRT) is an established treatment for patients with chronic heart failure. However, CRT-associated structural and functional remodeling at cellular subcellular levels only partly understood. Objective: To investigate the effects of CRT on structures protein distributions associated excitation-contraction coupling ventricular cardiomyocytes. Methods Results: Our studies revealed transverse tubular system (t-system) spatial association ryanodine...
The hypothesis that Na(+) influx during the action potential (AP) activates reverse Na(+)-Ca(2+) exchange (NCX) and subsequent entry of trigger Ca(2+) is controversial. We tested this by monitoring intracellular before after selective inactivation I(Na) prior to a simulated in patch-clamped ventricular myocytes isolated from adult wild-type (WT) NCX knockout (KO) mice. First, we inactivated using ramp prepulse 45 mV. In WT cells, decreased transient amplitude 51.1 +/- 4.6% (P < 0.001, n =...
Ca2+ transients were activated in rabbit ventricular cells by a sequence of action potential shaped voltage clamps. After activating series control transients, Na+ currents (INa) inactivated with ramp from -80 to -40 mV (1.5 s) prior the clamp. The detected calcium indicator Fluo-4 and an epifluorescence system. With zero pipette INa inactivation produced decline SR release flux (measured as maximum rate rise transient) 27 ± 4% (n = 9, P < 0.001) peak amplitude reduction 10 3% 0.05). 5 mm...
The transcriptional regulatory machinery in mitochondrial bioenergetics is complex and still not completely understood. We previously demonstrated that the histone methyltransferase Smyd1 regulates energetics. Here, we identified Perm1 (PPARGC-1 ESRR-induced regulator, muscle specific 1) as a downstream target of through RNA-seq. Chromatin immunoprecipitation assay showed directly interacts with promoter mouse heart, this interaction was significantly reduced hearts failing due to pressure...
Electrophysiological modeling of cardiac tissue is commonly based on functional and structural properties measured in experiments. Our knowledge these incomplete, particular their remodeling disease. Here, we introduce a methodology for quantitative characterization fluorescent labeling, 3-D scanning confocal microscopy, image processing reconstruction micro-structure at sub-micrometer resolution. We applied this to normal rabbit ventricular from hearts with myocardial infarction. analysis...
Key points Association of plasma membrane BK Ca channels with BK‐β subunits shapes their biophysical properties and physiological roles; however, functional modulation the mitochondrial channel (mitoBK ) by is not established. MitoBK ‐α regulatory BK‐β1 subunit associate in mouse cardiac mitochondria. A large fraction mitoBK display similar to that when associated (left‐shifted voltage dependence activation, V 1/2 = −55 mV, 12 µ m matrix 2+ ). In knockout mice, displayed a low P o...
Abstract The genetic architecture of atrial fibrillation (AF) encompasses low impact, common variants and high rare variants. Here, we characterize a impact AF-susceptibility allele, KCNQ1 R231H, describe its transcontinental geographic distribution history. Induced pluripotent stem cell-derived cardiomyocytes procured from risk allele carriers exhibit abbreviated action potential duration, consistent with gain-of-function effect. Using identity-by-descent (IBD) networks, estimate the broad-...
Journal Article Serum Antibodies to Heat-Labile Enterotoxin of Campylobacter jejuni Get access Guillermo M. Ruiz-Palacios, Ruiz-Palacios Department Infectious Diseases, Instituto Nacional de la Nutricion, Tlalpan, Mexico Search for other works by this author on: Oxford Academic PubMed Google Scholar Yolanda López-Vidal, López-Vidal Jesús Torres, Torres Norma The Volume 152, Issue 2, August 1985, Pages 413–416, https://doi.org/10.1093/infdis/152.2.413 Published: 01 1985 history Received: 17...
Abstract Aims Diminished repolarization reserve contributes to the arrhythmogenic substrate in many disease states. Pharmacological activation of K + channels has been suggested as a potential antiarrhythmic therapy such conditions. Having previously demonstrated that I K1 and Kr can modulate cardiac conduction, we tested here effects pharmacological KATP Ks on conduction its dependence sodium current ( Na ). Methods Results Bath electrocardiograms (ECGs) recorded from Langendorff‐perfused...
Calcium/calmodulin-dependent protein kinase II (CaMKII) regulates the principle ion channels mediating cardiac excitability and conduction, but how this regulation translates to normal ischemic heart remains unknown. Diverging results on CaMKII of Na+ further prevent predicting activity conduction in intact heart. To address deficiency, we tested effects blocker KN93 (1 2.75 μM) its inactive analog KN92 (2.75 left (LV) right (RV) ventricles rabbit hearts during perfusion global ischemia. We...
Aims: Autophagy is a catabolic process required for the maintenance of cardiac health. Insulin and insulin-like growth factor 1 (IGF-1) are potent inhibitors autophagy as such, one would predict that will be increased in insulin-resistant/diabetic heart. However, rather decreased hearts diabetic/insulin-resistant mice. The aim this study to determine contribution IGF-1 receptor signaling suppression insulin (IR)-deficient hearts. Results: Absence IRs heart was associated with reduced...
Abstract Aim To study the role of mitochondria in recovery guinea‐pig hearts exposed to high‐ K + ‐cardioplegia ( CPG ) and ischaemia/reperfusion I/R Methods We measured contractility heat release perfused cytosolic mitochondrial C a 2+ by epifluorescence confocal microscopy isolated cardiomyocytes loaded with F luo‐4 or R hod‐2. Results In hearts, increased postischaemic contractile recovery, this was potentiated m NCX blocker clonazepam KATP opener diazoxide, which also prevented fall...
The "stress" kinases cAMP-dependent protein kinase (PKA) and calcium/calmodulin-dependent II (CaMKII), phosphorylate the Na
<title>Abstract</title> There has been steady progress in documenting the psychosocial risk factors of hypertension. However, most extant evidence is based on population from developed countries. Using nationally representative data India, this cross-sectional study explores whether spousal age gap associated with hypertension married women aged 20 to 49 years. Based difference their husbands, were grouped into four categories: husband was – i) similar age, ii) 3–5 years older, iii) 6–9 and...
Background/Aims: Advances in induced pluripotent stem cell (iPSC) technology allow for reprogramming of adult somatic cells into from which patient- and disease-specific cardiomyocytes (CMs) can be derived. Yet, the potential iPSC to revolutionize cardiovascular research is limited, part, by embryonic nature these cells. Here, we test hypothesis that decellularized porcine left ventricular extracellular cardiac matrix (ECM) provides environmental cues promote transcriptional maturation...
Our prior work has shown that Na+ current (INa) affects sarcoplasmic reticular (SR) Ca2+ release by activating early reverse of the Na+-Ca2+ exchanger (NCX). The resulting entry primes dyadic cleft, which appears to increase channel coupling fidelity. It been skeletal isoform voltage-gated (Nav1.4) is main tetrodotoxin (TTX)-sensitive Nav expressed in adult rabbit ventricular cardiomyocytes. Here, I tested hypothesis it also principal involved priming mechanism. Action potentials (APs) were...