A5009922598- Cardiac electrophysiology and arrhythmias
- Cardiac Arrhythmias and Treatments
- Cardiac pacing and defibrillation studies
- Ion channel regulation and function
- Atrial Fibrillation Management and Outcomes
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Cardiac Structural Anomalies and Repair
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Adipose Tissue and Metabolism
- Cardiac Ischemia and Reperfusion
- Sepsis Diagnosis and Treatment
- Cardiac Imaging and Diagnostics
- Cardiac Valve Diseases and Treatments
- Cardiovascular Function and Risk Factors
- Cardiovascular Syncope and Autonomic Disorders
- Cardiovascular Issues in Pregnancy
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neuropharmacology Research
- Heart Rate Variability and Autonomic Control
- Mechanical Circulatory Support Devices
- Takotsubo Cardiomyopathy and Associated Phenomena
- SARS-CoV-2 and COVID-19 Research
- Pulmonary Hypertension Research and Treatments
Kliniken Maria Hilf
2018-2025
University of Münster
2014-2025
St. Franziskus Hospital
2024
University Hospital Münster
2016-2023
RWTH Aachen University
2021
Universitätsklinikum Aachen
2021
Paracelsus Medical University
2011-2017
University of Cologne
2003-2012
UCLA Health
2010
University of California, Los Angeles
2010
The hypothesis that Na(+) influx during the action potential (AP) activates reverse Na(+)-Ca(2+) exchange (NCX) and subsequent entry of trigger Ca(2+) is controversial. We tested this by monitoring intracellular before after selective inactivation I(Na) prior to a simulated in patch-clamped ventricular myocytes isolated from adult wild-type (WT) NCX knockout (KO) mice. First, we inactivated using ramp prepulse 45 mV. In WT cells, decreased transient amplitude 51.1 +/- 4.6% (P < 0.001, n =...
Pulmonary hypertension (PH) is associated with progressive impairment of right ventricular function, reduced exercise capacity and a poor prognosis. Little known about the prevalence, clinical manifestation impact atrial fibrillation (AF) on cardiac function in PH.In four year single-centre retrospective analysis 225 patients confirmed PH various origins were enrolled to investigate prevalence AF, assess manifestation, 6-minute walk distance, NT-proBNP levels, echocardiographic parameters...
Background— The pacemaker current I f contributes to spontaneous diastolic depolarization of cardiac autonomic cells. In heterologous expression, HCN channels exhibit a hyperpolarization-activated inward similar . However, the links between genes and native are largely inferential, it remains unknown whether is essential for pacing. Methods Results— To clarify this situation, we generated GYG 402–404 AYA pore mutation HCN2, which rendered channel nonfunctional suppressed wild-type HCN2 in...
Abstract Aims COVID‐19, a respiratory viral disease causing severe pneumonia, also affects the heart and other organs. Whether its cardiac involvement is specific feature consisting of myocarditis, or simply due to microvascular injury systemic inflammation, yet unclear presently debated. Because myocardial common in kinds pneumonias, we investigated compared such occurrence pneumonias COVID‐19 causes. Methods results We analysed data from 156 critically ill patients requiring mechanical...
With a high prevalence of coronary artery disease (CAD) among patients with atrial fibrillation (AF), CAD is one the main risk factors for AF. However, little known about characteristics in AF patients, especially whether specific anatomical distribution stenoses might predispose an individual to via ischemia remains speculative. To address this issue, we evaluated potential associations between angiographic and AF.In single-center retrospective analysis, 796 consecutive confirmed (CAD-AF)...
Background: In patients requiring dual-chamber pacemaker (DDD) implantation, optimal atrial lead position remains a matter of debate. While most centers prefer implantation in the right appendage (Non-BB-P), due to speculated favorable impact on conduction characteristics, often, Bachman bundle pacing (BB-P) is recommended. However, data investigating clinical outcomes these are still rare. Methods: To evaluate this issue, retrospective single-center study, one-year follow-up, interrogations...
To increase the supply, many countries harvest allograft valves from explanted hearts of transplant recipients with ischaemic (ICM) or dilated cardiomyopathy (DCM). This study determines structural integrity cardiomyopathic hearts.Extracellular matrix (ECM) was examined in human obtained normal, ICM, and DCM hearts. confirm if ECM changes were directly related to cardiomyopathy, we developed a porcine model chronic ICM. Histology immunohistostaining, as well non-invasive multiphoton second...
Ca2+ transients were activated in rabbit ventricular cells by a sequence of action potential shaped voltage clamps. After activating series control transients, Na+ currents (INa) inactivated with ramp from -80 to -40 mV (1.5 s) prior the clamp. The detected calcium indicator Fluo-4 and an epifluorescence system. With zero pipette INa inactivation produced decline SR release flux (measured as maximum rate rise transient) 27 ± 4% (n = 9, P < 0.001) peak amplitude reduction 10 3% 0.05). 5 mm...
The hyperpolarization-activated cyclic nucleotide-gated (HCN) current I(f)/I(HCN) is generally thought to be carried by Na(+) and K(+) under physiological conditions. Recently, Ca(2+) influx through HCN channels has indirectly been postulated. However, direct functional evidence of permeation still lacking.To possibly provide I(HCN)/I(f), we performed inside-out cell-attached single-channel recordings heterologously expressed native rat human I(f), since Ca(2+)-mediated currents may not...
The results of the recently published randomized SIMPLE trial question role routine intraoperative defibrillation testing. However, testing is still recommended during implantation entirely subcutaneous implantable cardioverter-defibrillator (S-ICD) system. To address whether in S-ICD systems necessary, we analyzed data a large, standard-of-care prospective single-center registry.In present study, 102 consecutive patients received an for primary (n=50) or secondary prevention (n=52)....
Introduction The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake) via the calcium uniporter (MCU) is highly controversial. Methods Thus, we analyzed mCa2+-uptake isolated cardiac mitochondria, MCU single-channel activity mitoplasts, dual Ca2+-transients from ((Ca2+)m) and intracellular compartment ((Ca2+)c) whole-cell configuration cardiomyocytes wild-type (WT) UCP2-/- mice. Results Isolated mitochondria showed a Ru360 sensitive mCa2+-uptake, which was...
New Findings What is the central question of this study? Knockdown UCP2 reduces mitochondrial Ca 2+ uptake. This suggests that Ucp2 knockout mice need to have additional effects on cytosolic handling prevent overload. However, specific mechanisms and their impact cardiac electrophysiology remain speculative. main finding its importance? In mice, decreased uptake compensated for by functional inhibition L‐type channels resultant shortening action potential duration. UCP2‐dependent modulations...
Severe COVID-19 pneumonia requiring intensive care treatment remains a clinical challenge to date. Dexamethasone was reported as promising option, leading reduction of mortality rates in severe disease. However, the effect dexamethasone on cardiac injury and pulmonary embolism largely elusive.In total 178 critically ill patients mechanical ventilation were recruited three European medical centres included present retrospective study. One hundred thirteen (63.5%) treated with for median...
Coxsackievirus B3 (CVB3) is known to induce acute and chronic myocarditis. Most infections are clinically unapparent but some patients suffer from ventricular arrhythmias (VA) sudden cardiac death (SCD). Studies showed that CVB3 infection may cause impaired function of ion channels, creating a proarrhythmic substrate. However, it unknown whether low level CVB3+ expression in myocytes altered electrophysiology leading VA.Cellular was used analyze cellular action potentials (APs) occurrence...
Takotsubo syndrome (TTS) is clinically indistinguishable from an acute coronary (ACS). In the absence of valid markers for differential diagnosis, angiography has been indispensable.
Background Caveolin-3 (cav-3) mutations are linked to the long-QT syndrome (LQTS) causing distinct clinical symptoms. Hyperpolarization-activated cyclic nucleotide channel 4 (HCN4) underlies pacemaker current I f. It associates with cav-3 and both form a macromolecular complex. Methods To examine effects of human LQTS-associated on HCN4-channel function, HEK293-cells were cotransfected HCN4 wild-type (WT) or mutant (T78M, A85T, S141R, F97C). currents recorded using whole-cell patch-clamp...
Gender-specific differences in the outcome of COVID-19 patients requiring intensive care treatment have been reported. However, a potential association with ICU therapy remains elusive.A total 224 consecutive (63 women) treated for severe disease mechanical ventilation were screened study. After propensity score matching gender, 40 men and women included Comparative analysis was conducted laboratory parameters, complications (pulmonary embolism, thrombosis, stroke, ventricular arrhythmias),...