A5017176137- Plant and Biological Electrophysiology Studies
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Molecular Communication and Nanonetworks
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Slime Mold and Myxomycetes Research
- Evolutionary Game Theory and Cooperation
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Electrochemical Analysis and Applications
- Nonlinear Dynamics and Pattern Formation
- Complex Systems and Time Series Analysis
- Biocrusts and Microbial Ecology
- Planarian Biology and Electrostimulation
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Microbial Metabolic Engineering and Bioproduction
- Force Microscopy Techniques and Applications
- Mycorrhizal Fungi and Plant Interactions
- Bacterial Genetics and Biotechnology
- Diffusion and Search Dynamics
University of Warwick
2015-2024
ARC Centre of Excellence in Synthetic Biology
2021-2023
Biotechnology Innovation Organization
2023
Google (United States)
2020
University of California, San Diego
2012
Ube Frontier University
2005-2011
Osaka University
2005-2011
Japan Science and Technology Agency
2010
Frontier Science Foundation
2009
From microbial biofilm communities to multicellular organisms, 3D macroscopic structures develop through poorly understood interplay between cellular processes and mechanical forces. Investigating wrinkled biofilms of Bacillus subtilis, we discovered a pattern localized cell death that spatially focuses forces, thereby initiates wrinkle formation. Deletion genes implicated in development, together with mathematical modeling, revealed ECM production underlies the localization death....
Membrane-potential dynamics mediate bacterial electrical signaling at both intra- and intercellular levels. Membrane potential is also central to cellular proliferation. It unclear whether the response external stimuli influenced by proliferative capacity. A new strategy enabling stimulation of bacteria with simultaneous monitoring single-cell membrane-potential would allow bridging this knowledge gap further extend electrophysiological studies into field microbiology. Here we report that an...
This paper reports on the use of scanning ion conductance microscopy (SICM) to locally map ionic properties and charge environment two live bacterial strains: Gram-negative Escherichia coli Gram-positive Bacillus subtilis. SICM results find heterogeneities across surface significant differences among bacteria. The bioelectrical B. subtilis was found be considerably more negatively charged compared E. coli. measurements, fitted a simplified finite element method (FEM) model, revealed values...
Self-organized multicellular behaviors enable cells to adapt and tolerate stressors a greater degree than isolated cells. However, whether how cellular communities alter their collective adaptively upon exposure stress is largely unclear. Here, we investigate this question using Bacillus subtilis, model system for bacterial multicellularity. We discover that, spatial gradient of kanamycin, swarming bacteria activate matrix genes transit biofilms. The initial stage transition underpinned by...
Abstract A key prerequisite to achieve a deeper understanding of microbial communities and engineer synthetic ones is identify the individual metabolic interactions among species how these are affected by different environmental factors. Deciphering physiological basis species–species species–environment in spatially organized environments requires reductionist approaches using ecologically functionally relevant species. To this end, we focus here on defined system study spatial context...
The last five decades of molecular and systems biology research have provided unprecedented insights into the genetic basis many cellular processes. Despite these insights, however, it is arguable that there still only limited predictive understanding cell behaviours. In particular, heterogeneity in single-cell behaviour initiation different metabolic, transcriptional or mechanical responses to environmental stimuli remain largely unexplained. To go beyond status quo , behaviours emerging...
As of July, 2020, acute respiratory syndrome caused by coronavirus COVID-19 is spreading over the world and causing severe economic damages. While minimizing human contact effective in managing outbreak, it causes losses. Strategies solving this dilemma considering interrelation between spread virus activities are urgent needs for mitigating health damage. Here we propose an abstract agent-based model outbreak which taken into account. The computational simulation recapitulated trade-off...
Abstract Recent studies have shown that bacterial membrane potential is dynamic and plays signaling roles. Yet, little still known about the mechanisms of dynamics regulation—owing to a scarcity appropriate research tools. Optical modulation could fill this gap provide new approach for studying controlling physiology electrical signaling. Here, authors show membrane‐targeted azobenzene ( Ziapin2 ) can be used photo‐modulate in cells Gram‐positive bacterium Bacillus subtilis . It found upon...
NUP98 and NUP214 form chimeric fusion proteins that assemble into phase-separated nuclear bodies containing CRM1, a export receptor. However, these bodies' function in controlling gene expression remains elusive. Here, we demonstrate the of NUP98::HOXA9 SET::NUP214 promote condensation mixed lineage leukemia 1 (MLL1), histone methyltransferase essential for maintenance HOX expression. These are robustly associated with MLL1/CRM1 co-localized on chromatin. Furthermore, whole-genome...
Nup98 is a mobile nucleoporin that forms distinct dots in the nucleus, and, although role for nuclear transport has been suggested, its precise function remains unclear. Here, we show plays an important Crm1-mediated protein export. Nuclear, but not cytoplasmic, of EGFP-tagged disappeared rapidly after cell treatment with leptomycin B, specific inhibitor export receptor, Crm1. Mutational analysis demonstrated physically and functionally interacts Crm1 RanGTP-dependent manner through...
Quality control of offspring is important for the survival cells. However, mechanisms by which quality cells may be checked while running genetic programs cellular differentiation remain unclear. Here we investigated during sporulating in Bacillus subtilis combining single-cell time-lapse microscopy, molecular biology, and mathematical modeling. Our results revealed that via premature germination coupled with electrical polarization outer membranes developing forespores. The forespores...
Oct4 is a member of the POU family transcription factors and plays critical role in both maintenance undifferentiated state embryonic stem (ES) cells reprogramming somatic to induced pluripotent cells. imported into nucleus where it functions as factor; however, spatiotemporal dynamic behavior remains largely unknown. In present study we show that nucleocytoplasmic shuttling protein. Furthermore, although mutants with altered nuclear import/export activity were able maintain self-renewal ES...
Membrane potential is a useful marker for antimicrobial susceptibility testing (AST) due to its fundamental roles in cell function. However, the difficulties associated with measuring membrane microbes restrict broad application. In this study, we present bioelectrical AST (BeAST) using model fungus
The nuclear pore complex (NPC) is the only gateway for molecular trafficking across envelope. NPC not merely a static nuclear-cytoplasmic transport gate; functional analysis of nucleoporins has revealed dynamic features in various cellular functions, such as mitotic spindle formation and protein modification. However, it known whether undergoes changes during biological processes cell differentiation. In present study, we evaluate expression levels several show that amount Nup358/RanBP2...
Npap60 (Nup50) is a nucleoporin that binds directly to importin alpha. In humans, there are two isoforms: the long (Npap60L) and short (Npap60S) forms. this study, we provide both in vitro vivo evidence Npap60L Npap60S function differently nuclear protein import. binding assays revealed stabilizes of alpha classical NLS-cargo, whereas promotes release NLS-cargo from time-lapse experiments showed when level controlled, allowing CAS efficiently promote dissociation Npap60/importin complex,...
Many species of soil fungi grow in the form branched networks that enable long-range communication and mass flow nutrient. These play important roles ecosystem as a major decomposer organic materials. While there have been investigations on branching fungal networks, their long-term growth dynamics space time is still not very well understood. In this study, we monitor spatio-temporal plant-promoting filamentous fungus Serendipita indica for several days controlled environment within...
Creating adaptive, sustainable, and dynamic biomaterials is a forthcoming mission of synthetic biology. Engineering spatially organized bacterial communities has potential to develop such bio-metamaterials. However, generating living patterns with precision, robustness, low technical barrier remains as challenge. Here we present an easily implementable technique for patterning live populations using controlled meniscus-driven fluidics system, named MeniFluidics. We demonstrate multiscale...