A5074195021- Pancreatitis Pathology and Treatment
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Pancreatic and Hepatic Oncology Research
- Dementia and Cognitive Impairment Research
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Erythrocyte Function and Pathophysiology
- Advanced Fluorescence Microscopy Techniques
- Healthcare Systems and Public Health
- Prion Diseases and Protein Misfolding
- Nuclear Receptors and Signaling
- Lipid metabolism and disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Parkinson's Disease Mechanisms and Treatments
- Liver Disease Diagnosis and Treatment
- Nerve injury and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Botulinum Toxin and Related Neurological Disorders
- Muscle and Compartmental Disorders
- Neurogenetic and Muscular Disorders Research
- Cerebrovascular and genetic disorders
- Kawasaki Disease and Coronary Complications
- Bacteriophages and microbial interactions
Instituto de Neurociencias
2010-2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2014-2023
Instituto de Medicina Genómica
2022
Instituto de investigación sanitaria y biomédica de Alicante
2022
Consejo Superior de Investigaciones Científicas
2005-2017
Hospital General Universitario de Alicante Doctor Balmis
2009-2014
Universitat de Miguel Hernández d'Elx
2006-2012
Universidad Autónoma de Madrid
1990-2011
Centro de Biología Molecular Severo Ochoa
2011
OBJECTIVES: Although aggressive fluid therapy during the first days of hospitalization is recommended by most guidelines and reviews on acute pancreatitis (AP), this recommendation not supported any direct evidence. We aimed to evaluate association between amount administered initial 24 h incidence organ failure (OF), local complications, mortality. METHODS: This was a prospective cohort study. included consecutive adult patients admitted with AP. Local complications OF were defined...
The amyloid precursor protein (APP) is a transmembrane glycoprotein that undergoes alternative proteolytic processing. Its processing through the amyloidogenic pathway originates large sAPPβ ectodomain fragment and β-amyloid peptide, while non-amyloidogenic generates sAPPα shorter non-fibrillar fragments. Hence, measuring has been proposed as means to identify imbalances between amyloidogenic/non-amyloidogenic pathways in brain of Alzheimer's disease (AD) patients. However, date, no...
Transmitted light images showed an intricate and dynamic cytoplasmic structural network in cultured bovine chromaffin cells observed under high magnification. These structures were sensitive to chemicals altering F-actin-myosin colocalised with peripheral F-actin, beta-actin myosin II. Interestingly, secretagogues induced a Ca2+-dependent, rapid (>10 second) transitory (60-second cycle) disassembling of these cortical structures. The simultaneous formation channel-like perpendicular the...
We have studied how the F-actin cytoskeleton is involved in establishing heterogeneous intracellular Ca2+ levels ([Ca2+]i) and organization of exocytotic machinery cultured bovine chromaffin cells. Simultaneous confocal visualization [Ca2+]i transmitted light studies showed that, following cell stimulation, maximal signal from Ca2+-sensitive fluorescent dye Fluo-3 was empty cytosolic spaces left by cytoskeletal cages. This mostly due to accumulation devoid components, as shown use...
Abstract This study assesses whether C-terminal fragments (CTF) of the amyloid precursor protein (APP) are present in cerebrospinal fluid (CSF) and their potential as biomarkers for Alzheimer’s disease (AD). Immunoprecipitation simultaneous assay by Western blotting using multiplex fluorescence imaging with specific antibodies against particular domains served to characterize CTFs APP human CSF. We demonstrate that APP-CTFs detectable CSF, being most abundant a 25-kDa fragment, probably...
Virus capsid assembly constitutes an attractive target for the development of antiviral therapies; a few experimental inhibitors this process HIV-1 and other viruses have been identified by screening compounds or selection from chemical libraries. As different, novel approach we undertaken rational design peptides that could act as competitive mimicking structural elements involved in intersubunit interfaces. Several discrete interfaces formation mature through polymerization protein CA were...
Soluble fragments of the amyloid precursor protein (APP) generated by α- and β-secretases, sAPPα sAPPβ, have been postulated as promising new cerebrospinal fluid (CSF) biomarkers for clinical diagnosis Alzheimer's disease (AD). However, capacity these soluble proteins to assemble has not explored could be relevant. Our aim is characterize possible sAPP oligomers that contribute quantification sAPPβ in CSF ELISA, well presence full-length APP (sAPPf). We employed co-immunoprecipitation,...
AIM:To study the activation of pancreatic and pulmonary mast cells effect cell inhibition on peritoneal alveolar macrophages during acute pancreatitis. METHODS:Pancreatitis was induced by intraductal infusion 5% sodium taurodeoxycholate in rats.The inhibitor cromolyn administered intraperitoneally (i.p.) 30 min before pancreatitis induction.The tissue damage evaluated histologically their state were evaluated.Peritoneal obtained expression tumor necrosis factor α determined.Myeloperoxidase...
The expression of SNAP‐25 fused to green fluorescent protein (GFP) has been instrumental in demonstrating SNARE role exocytosis. wild‐type GFP–SNAP‐25 and a Δ9 form, product botulinum neurotoxin A activity, the main ingredient BOTOX preparation, were employed here study implication vesicle mobility fusion cultured bovine chromaffin cells, neuroendocrine exocytotic model. Using total internal reflection microscopy, we have identified membrane microdomains 500–600 nm diameter that contain both...
Abstract Background Members of the low-density lipoprotein (LDL) receptor family are involved in endocytosis and transducing signals, but also amyloid precursor protein (APP) processing β-amyloid secretion. ApoER2/LRP8 is a member this with key roles synaptic plasticity adult brain. ApoER2 cleaved after binding its ligand, reelin protein, generating an intracellular domain (ApoER2-ICD) that modulates gene transcription itself. We have analyzed whether ApoER2-ICD able to regulate expression...
Reelin binds to the apolipoprotein E receptor apoER2 activate an intracellular signaling cascade. The proteolytic cleavage of reelin follows binding but can also occur independently its receptors. This study assesses whether fragments are differentially affected in cerebrospinal fluid (CSF) Alzheimer’s disease (AD) subjects. CSF species were analyzed by Western blotting, employing antibodies against N- and C-terminal domains. In AD patients, we found a decrease 420 kDa full-length compared...
Cultured bovine chromaffin cells have been used extensively as a neuroendocrine model to study regulated secretion. In order extend such experimental findings the physiological situation, it is necessary mayor cellular structures affecting secretion in cultured with their counterparts present adrenomedullary tissue. F-actin concentrates peripheral ring cells, witnessed by phalloidin-rodhamine labelling, while extends throughout cytosol native cells. This result also confirmed when studying...
Neuroendocrine chromaffin cells were used to study the mechanism of snake phospholipase A2 (PLA2) neurotoxin enhancement exocytosis. Notexin, beta-bungarotoxin, taipoxin or textilotoxin enhanced fast release catecholamines elicited by flash photolysis cytosolic caged calcium. Such an increase correlates with capacity these neurotoxins cause fragmentation F-actin cortical barrier subsequent accumulation vesicles in proximity plasma membrane. These PLA2 do not act via protein kinase C...