A5030026948- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Apelin-related biomedical research
- Phagocytosis and Immune Regulation
- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- Cancer Mechanisms and Therapy
- Neuroscience and Neural Engineering
- Neuropeptides and Animal Physiology
- Proteoglycans and glycosaminoglycans research
- Functional Brain Connectivity Studies
- Complement system in diseases
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- Neural dynamics and brain function
University of North Carolina at Chapel Hill
2016-2020
Institute of Molecular Biology and Biophysics
2019
Vanderbilt University
2012-2014
John F. Kennedy Center for the Performing Arts
2012
During the development of peripheral nervous system there is extensive apoptosis, and these neuronal corpses need to be cleared prevent an inflammatory response. Recently, Jedi-1 MEGF10, both expressed in glial precursor cells, were identified mouse as having essential role this phagocytosis (Wu et al., 2009); however, mechanisms by which they promote engulfment remained unknown. Both MEGF10 are homologous Drosophila melanogaster receptor Draper, mediates through activation tyrosine kinase...
Perineuronal nets (PNNs) are implicated in closure of critical periods synaptic plasticity the brain, but molecular mechanisms by which PNNs regulate synapse development obscure. A receptor complex NCAM and EphA3 mediates postnatal remodeling inhibitory perisomatic synapses GABAergic interneurons onto pyramidal cells mouse frontal cortex necessary for excitatory/inhibitory balance. Here it is shown that enzymatic removal PNN glycosaminoglycan chains decreased density organotypic cortical...
Neuron-glial related cell adhesion molecule NrCAM is a newly identified negative regulator of spine density that genetically interacts with Semaphorin3F (Sema3F), and implicated in autism spectrum disorders (ASD). To investigate role for pruning during the critical adolescent period when networks are established, we generated novel conditional, inducible mutant mice (Nex1Cre-ERT2: NrCAMflox/flox). We demonstrate functions autonomously adolescence pyramidal neurons to restrict visual (V1)...
Dendritic spines in the developing mammalian neocortex are initially overproduced and then eliminated during adolescence to achieve appropriate levels of excitation mature networks. We show here that L1 family cell adhesion molecule Close Homolog (CHL1) secreted repellent ligand Semaphorin 3B (Sema3B) function together induce dendritic spine pruning cortical pyramidal neurons. Loss CHL1 null mutant mice both genders resulted increased density a greater proportion immature on apical dendrites...
Establishment of a proper balance excitatory and inhibitory connectivity is achieved during development cortical networks adjusted through synaptic plasticity. The neural cell adhesion molecule (NCAM) the receptor tyrosine kinase EphA3 regulate perisomatic synapse density GABAergic interneurons in mouse frontal cortex ephrin-A5-induced growth cone collapse. In this study, it was demonstrated that binding NCAM occurred between Ig2 domain cysteine-rich (CRD). interface further refined...
During the development of peripheral nervous system, large number apoptotic neurons generated are phagocytosed by glial precursor cells. This clearance is mediated, in part, through mammalian engulfment receptor Jedi-1. However, mechanisms which Jedi-1 mediates phagocytosis poorly understood. Here we demonstrate that associates with GULP, homologue CED-6, an adaptor protein required for mediated nematode CED-1. Silencing GULP or mutating NPXY motif Jedi-1, binding, prevents Jedi-1-mediated...
The neural cell adhesion molecule, NCAM, has been shown to be an obligate regulator of synaptic stability and pruning during critical periods cortical maturation. However, the functional consequences NCAM deletion on organization inhibitory circuits in cortex are not known. In VGAT-channelrhodopsin2-EYFP transgenic mice, is expressed postnatally at perisomatic puncta EYFP-labeled parvalbumin, somatostatin calretinin-positive interneurons, neuropil anterior cingulate cortex. To investigate...
Parvalbumin (PV)-expressing basket interneurons in the prefrontal cortex (PFC) regulate pyramidal cell firing, synchrony, and network oscillations. Yet, it is unclear how their perisomatic inputs to neurons are integrated into neural circuitry adjusted postnatally. Neural adhesion molecule NCAM expressed a variety of cells PFC cooperates with EphrinA/EphAs inhibitory synapse density. Here, analysis novel parvalbumin (PV)-Cre: F/F mouse mutant revealed that functions presynaptically PV+...