A5030773328- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Bioinformatics and Genomic Networks
- Caveolin-1 and cellular processes
- CRISPR and Genetic Engineering
- Nitric Oxide and Endothelin Effects
- Chromosomal and Genetic Variations
- Asthma and respiratory diseases
- Neuroblastoma Research and Treatments
- Computational Drug Discovery Methods
- Plant Virus Research Studies
- Neurofibromatosis and Schwannoma Cases
- Complement system in diseases
- RNA and protein synthesis mechanisms
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Estrogen and related hormone effects
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
Vanderbilt Health
2018
Vanderbilt University
2014-2018
Vanderbilt University Medical Center
2015
University of Washington
2012
Georgetown University
2010-2012
Georgetown University Medical Center
2011
Davidson College
2010
Florida Agricultural and Mechanical University
1998
EGF receptor (EGFR) is a critical signaling node throughout life. However, it has not been possible to directly visualize endogenous Egfr in mice. Using CRISPR/Cas9 genome editing, we appended fluorescent reporter the C terminus of Egfr. Homozygous mice appear normal and EGFR intact vitro vivo. We detect distinct patterns expression progenitor differentiated compartments embryonic adult Systemic delivery or amphiregulin results markedly different internalization trafficking hepatocytes. In...
Lack of understanding endocrine resistance remains one the major challenges for breast cancer researchers, clinicians, and patients. Current reductionist approaches to molecular signaling driving have offered mostly incremental progress over past 10 years. As field systems biology has begun mature, network modeling tools being developed applied therein offer a different way think about how regulation critical cellular functions are integrated. To gain novel insights, we first describe some...
Neuroblastoma is a pediatric malignancy of the sympathetic ganglia and adrenal glands, hypothesized to originate from progenitors developing nervous system. Amplification MYCN oncogene genetic marker risk in this disease. Understanding impact expression on sympathoadrenal progenitor development may improve our knowledge neuroblastoma initiation progression. We isolated cells postnatal murine gland by sphere culture found them be multipotent, generating differentiated colonies neurons,...
During the development of peripheral nervous system, large number apoptotic neurons generated are phagocytosed by glial precursor cells. This clearance is mediated, in part, through mammalian engulfment receptor Jedi-1. However, mechanisms which Jedi-1 mediates phagocytosis poorly understood. Here we demonstrate that associates with GULP, homologue CED-6, an adaptor protein required for mediated nematode CED-1. Silencing GULP or mutating NPXY motif Jedi-1, binding, prevents Jedi-1-mediated...
The p75 neurotrophin receptor (p75NTR) plays an integral role in patterning the sympathetic nervous system during development. Initially, p75NTR is expressed at low levels as axons project toward their targets, which enables neurotrophin-3 (NT3) to activate TrkA receptors and promote growth. Upon reaching nerve growth factor (NGF) producing tissues, upregulated, resulting formation of TrkA-p75 complexes, are high-affinity binding sites selective for NGF, thereby blunting NT3 signaling. level...
Temporal coordination of meiosis with spermatid morphogenesis is crucial for successful generation mature sperm cells. We identified a recessive male sterile Drosophila melanogaster mutant, mitoshell, in which events are initiated too early, before meiotic onset. Premature mitochondrial aggregation and fusion lead to an aberrant shell around premeiotic nuclei. Despite karyokinesis, improper localization mitoshell testes associated defective astral central spindles lack contractile rings,...
Abstract Most of the 192,000 newly diagnosed cases invasive breast cancer in 2011 will be estrogen receptor positive alpha (ER+). Endocrine therapy, administered as an antiestrogen, e.g., Tamoxifen (TAM) or Faslodex (Fulvestrant; ICI 182,780) aromatase inhibitor (AI), Letrozole (LET), is least toxic and most effective means to manage hormone-dependent such patients. However, resistance endocrine therapy remains a significant clinical problem. Our group has shown that antiestrogen resistant...
Abstract Most of the 178,000 newly diagnosed cases invasive breast cancer in 2010 will be estrogen receptor positive α (ER+). For these patients, endocrine therapy, administered as an antiestrogen, e.g., Tamoxifen (TAM) or Faslodex (Fulvestrant; ICI 182,780) aromatase inhibitor (AI), Letrozole (LET), is least toxic and most effective means to manage their hormone-dependent cancer. While 5 years produces a 26% proportional reduction mortality, advanced ER+ largely remains incurable disease...