A5008154132- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Tuberous Sclerosis Complex Research
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Cell Image Analysis Techniques
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Hedgehog Signaling Pathway Studies
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Cancer Immunotherapy and Biomarkers
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- Radiomics and Machine Learning in Medical Imaging
- CRISPR and Genetic Engineering
- Axon Guidance and Neuronal Signaling
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- RNA modifications and cancer
Vanderbilt University
2016-2025
Vanderbilt University Medical Center
2016-2025
Neurological Surgery
2016-2025
University of Colorado Anschutz Medical Campus
2025
Vanderbilt Health
2020-2024
Meharry Medical College
2022-2023
Vanderbilt-Ingram Cancer Center
2020-2023
Massachusetts General Hospital
2023
Breast Cancer Research Foundation
2023
Allen Institute for Brain Science
2023
Mass cytometry measures 36 or more markers per cell and is an appealing platform for comprehensive phenotyping of cells in human tissue tumor biopsies. While disaggregation fluorescence protocols were pioneered decades ago, it not known whether established will be effective mass maintain cancer stromal diversity.Tissue preparation techniques systematically compared gliomas melanomas, patient derived xenografts small lung cancer, tonsil as a control. Enzymes assessed included DNase, HyQTase,...
Abstract Tuberous Sclerosis Complex (TSC) is a debilitating developmental disorder characterized by variety of clinical manifestations. While benign tumors in the heart, lungs, kidney, and brain are all hallmarks disease, most severe symptoms TSC often neurological, including seizures, autism, psychiatric disorders, intellectual disabilities. caused loss function mutations TSC1 or TSC2 genes consequent dysregulation signaling via mechanistic Target Rapamycin 1 (mTORC1). neurological...
One of the enigmas in tumor biology is that different types cancers are prevalent age groups. possible explanation ability a specific oncogene to cause tumorigenesis particular cell type depends on epigenetic parameters such as developmental context. To address this hypothesis, we have used tetracycline regulatory system generate transgenic mice which expression c-MYC human transgene can be conditionally regulated murine hepatocytes. MYC's induce was dependent upon In embryonic and neonatal...
Highlights•High Gli1 expression appears transiently in the dorsal V-SVZ during early postnatal ages•Gli1-expressing RGCs subcallosal generate many oligodendroglial cells•Oligodendrogenesis is SHH dependent•Ectopic activation of SMO increases oligodendrogenesis neonatal and adult miceSummaryNeural stem cells different locations mouse ventricular-subventricular zone (V-SVZ) subtypes olfactory bulb (OB) interneurons. High Sonic hedgehog (SHH) signaling ventral regulates production specific...
Although malignant astrocytomas are a leading cause of cancer-related death in children, rational therapeutic strategies lacking. We previously identified activating mutations v-raf murine sarcoma viral oncogene homolog B1 ( BRAF ) T1799A encoding V600E association with homozygous cyclin-dependent kinase inhibitor 2A CDKN2A , p14ARF and p16Ink4a deletions pediatric infiltrative astrocytomas. Here we report that expression neural progenitors (NPs) is insufficient for tumorigenesis increases...
Background: Mass cytometry measures 36 or more markers per cell and is an appealing platform for comprehensive phenotyping of cells in human tissue tumor biopsies. While disaggregation fluorescence protocols were pioneered decades ago, it not known whether established will be effective mass maintain cancer stromal diversity. Methods: Tissue preparation techniques systematically compared gliomas melanomas, patient derived xenografts small lung cancer, tonsil as a control. Enzymes assessed...
Abstract Mass cytometry is a single‐cell biology technique that samples >500 cells per second, measures >35 features cell, and sensitive across dynamic range of >10 4 relative intensity units feature. This combination technical assets has powered series recent cytomic studies where investigators used mass to measure protein phospho‐protein expression in millions cells, characterize rare cell types healthy diseased tissues, reveal novel, unexpected cells. However, these advances...
EGF receptor (EGFR) is a critical signaling node throughout life. However, it has not been possible to directly visualize endogenous Egfr in mice. Using CRISPR/Cas9 genome editing, we appended fluorescent reporter the C terminus of Egfr. Homozygous mice appear normal and EGFR intact vitro vivo. We detect distinct patterns expression progenitor differentiated compartments embryonic adult Systemic delivery or amphiregulin results markedly different internalization trafficking hepatocytes. In...
Glioblastoma (GBM), the most aggressive adult brain cancer, comprises a complex tumour microenvironment (TME) with diverse cellular interactions driving progression and pathobiology. How these spatial patterns evolve treatment remains unclear. Here, we apply imaging mass cytometry to analyse protein-level changes in paired pre- post-treatment GBM samples from five patients. We find significant increase normal cells alongside reduction vascular cells. Moreover, despite minimal overall change...
Abstract Whether patients with glioblastoma that contacts the ventricular-subventricular zone stem cell niche (VSVZ + GBM) have a distinct survival profile from VSVZ − GBM independent of other known predictors or molecular profiles is unclear. Using multivariate Cox analysis to adjust for widely-accepted predictors, hazard ratios (HRs) overall (OS) and progression free (PFS) between were calculated in 170 single-institution 254 included both The Cancer Genome (TCGA) Imaging (TCIA) atlases....
A goal of cancer research is to reveal cell subsets linked continuous clinical outcomes generate new therapeutic and biomarker hypotheses. We introduce a machine learning algorithm, Risk Assessment Population IDentification (RAPID), that unsupervised automated, identifies phenotypically distinct populations, determines whether these populations stratify patient survival. With pilot mass cytometry dataset 2 million cells from 28 glioblastomas, RAPID identified tumor whose abundance...
Characterizing and quantifying cell types within glioblastoma (GBM) tumors at scale will facilitate a better understanding of the association between cellular landscape tumor phenotypes or clinical correlates. We aimed to develop tool that deconvolutes immune neoplastic cells GBM microenvironment from bulk RNA sequencing data.