A5011411184- Insect Resistance and Genetics
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- Entomopathogenic Microorganisms in Pest Control
- Insect Pest Control Strategies
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Genetically Modified Organisms Research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Agricultural pest management studies
- Research on scale insects
- Molecular Biology Techniques and Applications
- Traditional and Medicinal Uses of Annonaceae
- Entomological Studies and Ecology
- Environmental DNA in Biodiversity Studies
- Plant and animal studies
- Environmental Toxicology and Ecotoxicology
- Pesticide Exposure and Toxicity
- Hemiptera Insect Studies
- Aquatic Invertebrate Ecology and Behavior
- Enzyme Catalysis and Immobilization
- Insect and Arachnid Ecology and Behavior
- Agricultural and Food Sciences
University of Nebraska–Lincoln
2015-2024
Universidade Tecnológica Federal do Paraná
2018
Universidad Autónoma de Bucaramanga
2015
Abstract Transgenic maize, Zea maize L., expressing the Cry1F protein from Bacillus thuringiensis has been registered for Spodoptera frugiperda (J. E. Smith) control since 2003. Unexpected damage to was reported in 2006 Puerto Rico and resistance S. documented. The inheritance of characterized a resistant strain originating Rico, which displayed >289-fold purified Cry1F. Concentration–response bioassays reciprocal crosses susceptible parental populations indicated that is recessive...
The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism its in many insects remains to be understood. In this study, we evaluated roles endocytic pathway genes Clathrin heavy chain (Chc), adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H+ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 putative sid-1-like (silA silC) response western corn rootworm (WCR) using a two-stage bioassay. Silencing Chc, Vha16, AP50 led...
Abstract Transgenic corn, Zea mays L., expressing the B acillus thuringiensis Berliner ( Bt ) protein Cry1F has been registered for S podoptera frugiperda (J. E. Smith) control since 2003 in USA . Unexpected damage to corn was reported 2006 Puerto Rico, and resistance from Rico documented. The study of fitness costs associated with insect insecticidal proteins is important understanding evolution evaluating management practices used mitigate transgenic corn. Currently, no studies have...
RNA interference (RNAi) is being developed as a potential tool for insect pest management. Increased understanding of the RNAi pathway in target pests will provide information to use this technology effectively and inform decisions related resistant management strategies based traits. Dicer 2 (Dcr2), an endonuclease responsible formation small interfering RNA's Argonaute (Ago2), essential catalytic component RNA-induced silencing complex (RISC) have both been associated with number different...
Abstract BACKGROUND Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is one of the major pests maize and in same feeding guild as noctuid Helicoverpa zea (Boddie) armigera (Hübner), recently reported South North America. The intraguild interactions these species were assessed laboratory field conditions by determining survival larvae interaction scenarios with non‐ Bt silks ears. Moreover, a video tracking system was utilized to evaluate behavioral parameters during larval or...
Abstract Background RNA interference (RNAi) triggered by maize plants expressing hairpins against specific western corn rootworm (WCR) transcripts have proven to be effective at controlling this pest. To provide robust crop protection, mRNA targeted double‐stranded must sensitive knockdown and encode essential proteins. Results Using WCR adult feeding assays, we identified Sec23 as a highly lethal RNAi target. encodes coatomer protein, component of the coat protein (COPII) complex that...
Abstract Coffee production is a global industry valued at approximately 173 billion US dollars. One of the main challenges facing coffee management berry borer (CBB), Hypothenemus hampei , which considered primary arthropod pest worldwide. Current control strategies are inefficient for CBB management. Although biotechnological alternatives, including RNA interference (RNAi), have been proposed in recent years to insect pests, characterizing genetics target essential successful application...
Parental RNAi (pRNAi) is an RNA interference response where the gene knockdown phenotype observed in progeny of treated organism. pRNAi has been demonstrated female western corn rootworms (WCR) via diet applications and described as a potential approach for rootworm pest management. However, it not clear if plant-expressed can provide effective control next generation WCR larvae field. In this study, we evaluated parameters required to generate successful genes brahma hunchback. The tested...
RNA interference (RNAi) is a naturally occurring gene silencing mechanism conserved across organisms with clearly defined cell nucleus (eukaryotes). Gene by RNAi through the degradation of target messenger (mRNA) has historically been used as research tool to study function genes. Over past two decades, vital genes explored for agricultural applications, including managing plant insect pests and pathogens, improving agronomic traits, increasing consumer desirability food. Using crop...
Abstract Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated performance six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin) , β-tubulin (Tubulin) elongation factor 1 alpha ( EF α ), glyceraldehyde-3 phosphate dehydrogenase GAPDH 40 S ribosomal protein S9 RpS9 ) ubiquitin-conjugating Ubi under different...
Abstract Insecticide exposure has been identified as a contributing stressor to the decline in North American monarch butterfly Danaus plexippus L. (Lepidoptera: Nymphalidae) population. Monarch toxicity data are currently limited and available focuses on lethal endpoints. This study examined 72-h of two pyrethroid insecticides, bifenthrin β-cyfluthrin, their effects growth diet consumption. The caterpillars was lower than β-cyfluthrin after 72 h. Survival most sensitive endpoint for...
RNA interference is a powerful tool against corn rootworm. Adults and neonates of southern rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), were exposed to the LC50 vATPase-A Snf7 double-stranded RNAs (dsRNAs), effects on female fecundity, egg viability, male fitness as measured by sperm viability mating capacity, larval recovery along with dry weight, instar determination 10 d after exposure dsRNA, determined. Significant reductions observed for number...
Abstract Understanding the behavior of pests targeted with B acillus thuringiensis erliner (Bt) crops is important to define resistance management strategies. Particularly study larval movement between plants determine feasibility refuge configurations. Exposure Bt maize, Z ea mays L . ( P oaceae), has been suggested increase in lepidopteran species but few studies have examined potential for interact behavioral responses toxins. Choice and no‐choice experiments were conducted S podoptera...
Striacosta albicosta (Smith) is a maize pest that has recently expanded its geographical range into the eastern United States and southeastern Canada. Aerial application of pyrethroids, such as bifenthrin, been major practice adopted to manage this pest. Reports field failure pyrethroids have increased since 2013. populations were collected in 2016 2017 from fields Nebraska, Kansas, Canada screened with bifenthrin active ingredient larval contact dose-response bioassays. Resistance ratios...