A5081574552- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Lipids, and Metabolism
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Gallbladder and Bile Duct Disorders
- Endoplasmic Reticulum Stress and Disease
- Cancer Genomics and Diagnostics
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Cancer Diagnosis and Treatment
- Breast Implant and Reconstruction
- Immune Cell Function and Interaction
- Periodontal Regeneration and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Single-cell and spatial transcriptomics
University of British Columbia
2021-2024
Hyperinsulinemia is independently associated with increased risk and mortality of pancreatic cancer. We recently reported that genetically reduced insulin production resulted in ~ 50% suppression intraepithelial neoplasia (PanIN) precancerous lesions mice. However, only female mice remained normoglycemic, the gene dosage rodent-specific Ins1 alleles was tested our previous model. Moreover, we did not delve into molecular cellular mechanisms modulating hyperinsulinemia.We studied how Ins2...
Abstract A central goal of physiological research is the understanding cell-specific roles disease-associated genes. Cre-mediated recombineering tool choice for cell type–specific analysis gene function in preclinical models. In type 1 diabetes (T1D) field, multiple lines nonobese diabetic (NOD) mice have been engineered to express Cre recombinase pancreatic β cells using insulin promoter fragments, but tissue promiscuity remains a concern. Constitutive Ins1tm1.1(cre)Thor (Ins1Cre) on...
Summary The rising pancreatic cancer incidence due to obesity and type 2 diabetes is closely tied hyperinsulinemia, an independent risk factor. Previous studies demonstrated reducing insulin production suppressed intraepithelial neoplasia (PanIN) pre-cancerous lesions in Kras -mutant mice. However, the pathophysiological molecular mechanisms remained unknown particular it was unclear whether hyperinsulinemia affected PanIN precursor cells directly or indirectly. Here, we demonstrate that...
Abstract The rising incidence of pancreatic cancer is largely driven by increased prevalence obesity and type 2 diabetes (T2D). Hyperinsulinemia a cardinal feature T2D, associated with mortality. Whether insulin alone plays causal role in increasing risk directly affecting the tumor cell origin remains unclear. Our previous studies demonstrated that genetically reducing production suppressed formation intraepithelial neoplasia (PanIN) pre-cancerous lesions mice mutant Kras. However,...
Background: Complete resection followed by adjuvant chemotherapy is the gold standard for patients with localized cholangiocarcinoma (CC) or gallbladder cancer (GBC). However, this not always feasible, and recurrence rates remain high. Objectives: To understand real-world proportions reason treatment failure in resected biliary tract cancers. Design methods: We performed a retrospective population-based review of GBC CC [intrahepatic (IHCC) extrahepatic (EHCC)] between 2005 2019 using BC...
505 Background: Complete resection followed by adjuvant chemotherapy is standard of care for patients with localized cholangiocarcinoma (CC) or gallbladder cancer (GBC) but not always feasible and recurrence rates remain high. Understanding the exact proportions reasons treatment failure important to design new approaches, data regarding this information scarce. Methods: We performed a retrospective population-based review GBC CC (intrahepatic (ICC) extrahepatic (ECC)) resected between...
522 Background: While only accounting for 3% of gastrointestinal malignancies, cholangiocarcinoma (CC) has poor prognosis. New targeted therapies are on the horizon and understanding potential size population eligible such is importance. Contemporary incidence outcomes in Canada have not been studied. Methods: Patients diagnosed with CC between 2005-2019 British Columbia were identified using BC Cancer Registry. Patient characteristics, treatments received collected analyzed. Results: A...
Abstract A central goal of physiological research is the understanding cell-specific roles disease-associated genes. Cre-mediated recombineering tool choice for cell type-specific analysis gene function in pre-clinical models. In type 1 diabetes field, multiple lines NOD mice have been engineered to express Cre recombinase pancreatic β-cells using insulin promoter fragments, but tissue promiscuity remains a concern. Constitutive Ins1 tm1.1(cre)Thor ( ) on C57/bl6-J background has high β-cell...
Abstract Hyperinsulinemia is a cardinal feature shared by both obesity and type 2 diabetes, independently associated with increased risk of pancreatic ductal adenocarcinoma (PDAC). We previously showed ~50% reduction in intraepithelial neoplasia (PanIN) pre-cancerous lesions mice genetically reduced insulin production. Our single-cell transcriptomic data suggested that many cell types could mediate the effects local hyperinsulinemia on PanIN development. In acinar cells from insulin, we...